Anti-RAP1/TERF2IP抗体[4c8/1] (ab14404)
![Western blot - Anti-RAP1/TERF2IP antibody [4c8/1] (ab14404)](https://www.abcam.cn/ps/products/14/ab14404/Images/ab14404-21908-anti-rap1-antibody-4c8-1-western-blot.jpg "Western blot - Anti-RAP1/TERF2IP antibody [4c8/1] (ab14404)")
Key features and details
- Mouse monoclonal [4c8/1] to RAP1/TERF2IP
- Suitable for: IP, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-RAP1/TERF2IP抗体[4c8/1]
参阅全部 RAP1/TERF2IP 一抗 -
描述
小鼠单克隆抗体[4c8/1] to RAP1/TERF2IP -
宿主
Mouse -
经测试应用
适用于: IP, WB, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human RAP1/TERF2IP.
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阳性对照
- In Western Blot, this antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293; HepG2; A431.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
4c8/1 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG2b -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14404于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use a concentration of 5 µg/ml.
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| WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
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| Flow Cyt |
| 说明 |
|---|
|
IP
Use a concentration of 5 µg/ml. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. |
靶标
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功能
Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'-TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes. -
组织特异性
Ubiquitous. Highly expressed. -
序列相似性
Belongs to the RAP1 family.
Contains 1 BRCT domain.
Contains 1 Myb-like domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. Cytoplasm. Chromosome. Chromosome > telomere. Associates with chromosomes, both at telomeres and in extratelomeric sites. Also exists as a cytoplasmic form, where it associates with the IKK complex. - Information by UniProt
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数据库链接
- Entrez Gene: 54386 Human
- Omim: 605061 Human
- SwissProt: Q9NYB0 Human
- Unigene: 301419 Human
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别名
- Dopamine receptor interacting protein 5 antibody
- Dopamine receptor-interacting protein 5 antibody
- DRIP 5 antibody
see all
图片
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Western blot - Anti-RAP1/TERF2IP antibody [4c8/1] (ab14404)All lanes : Anti-RAP1/TERF2IP antibody [4c8/1] (ab14404) at 5 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes -
![Immunoprecipitation - Anti-RAP1/TERF2IP antibody [4c8/1] (ab14404)](https://www.abcam.cn/ps/products/14/ab14404/Images/ab14404-21907-anti-rap1-antibody-4c8-1-immunoprecipitation.jpg)
Immunoprecipitation - Anti-RAP1/TERF2IP antibody [4c8/1] (ab14404)RAP1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to RAP1/TERF2IP RITM0035008 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab14404.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 55kDa; RAP1 -
![Flow Cytometry - Anti-RAP1/TERF2IP antibody [4c8/1] (ab14404)](https://www.abcam.cn/ps/products/14/ab14404/Images/ab14404-21906-anti-rap1-antibody-4c8-1-flow-cytometry.jpg)
Flow Cytometry - Anti-RAP1/TERF2IP antibody [4c8/1] (ab14404)Overlay histogram showing HeLa cells stained with ab14404 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14404, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab14404 被引用在 8 文献中.
- Li H et al. Secreted LRPAP1 binds and triggers IFNAR1 degradation to facilitate virus evasion from cellular innate immunity. Signal Transduct Target Ther 8:374 (2023). PubMed: 37743411
- Zhang G et al. A Novel Molecular Classification Method for Glioblastoma Based on Tumor Cell Differentiation Trajectories. Stem Cells Int 2023:2826815 (2023). PubMed: 37964983
- Xu Q et al. Vascular senescence in progeria: role of endothelial dysfunction. Eur Heart J Open 2:oeac047 (2022). PubMed: 36117952
- Pennarun G et al. Increase in lamin B1 promotes telomere instability by disrupting the shelterin complex in human cells. Nucleic Acids Res 49:9886-9905 (2021). PubMed: 34469544
- Zhang B et al. Arecoline Enhances Phosphodiesterase 4A Activity to Promote Transforming Growth Factor-β-Induced Buccal Mucosal Fibroblast Activation via cAMP-Epac1 Signaling Pathway. Front Pharmacol 12:722040 (2021). PubMed: 34819854
- Pereira CD et al. Nuclear Accumulation of LAP1:TRF2 Complex during DNA Damage Response Uncovers a Novel Role for LAP1. Cells 9:N/A (2020). PubMed: 32751253
- Liu H et al. NLRP3 inflammasome induced liver graft injury through activation of telomere-independent RAP1/KC axis. J Pathol 242:284-296 (2017). PubMed: 28378341
- Wang J et al. Vascular Smooth Muscle Cell Senescence Promotes Atherosclerosis and Features of Plaque Vulnerability. Circulation 132:1909-19 (2015). PubMed: 26416809