重组Anti-RAP1A抗体[EP878] (ab124963)
![Western blot - Anti-RAP1A antibody [EP878] (ab124963)](https://www.abcam.cn/ps/products/124/ab124963/Images/ab124963-550823-anti-rap1a-antibody-ep878-western-blot-human-mouse-rat.jpg "Western blot - Anti-RAP1A antibody [EP878] (ab124963)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP878] to RAP1A
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-RAP1A抗体[EP878]
参阅全部 RAP1A 一抗 -
描述
兔单克隆抗体[EP878] to RAP1A -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human brain, BXPC 3, C6, and NIH 3T3 cell lysates.
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常规说明
This product has switched from a hybridoma to recombinant production method on 26th August 2024.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP878 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124963于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
靶标
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功能
Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. -
序列相似性
Belongs to the small GTPase superfamily. Ras family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5906 Human
- Entrez Gene: 109905 Mouse
- Entrez Gene: 295347 Rat
- Omim: 179520 Human
- SwissProt: P62834 Human
- SwissProt: P62835 Mouse
- SwissProt: P62836 Rat
- Unigene: 190334 Human
see all -
别名
- C21KG antibody
- G 22K antibody
- G-22K antibody
see all
图片
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Western blot - Anti-RAP1A antibody [EP878] (ab124963)All lanes : Anti-RAP1A antibody [EP878] (ab124963) at 1/1000 dilution
Lane 1 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaExposure time: Lane 1, 10 seconds; Lane 2, 60 seconds; Lane 3, 5 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab124963 尚未被引用在任何文献中。