Name: Anti-RANTES antibody
SKU: ab9679
Rating: 4 (8 reviews)

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Sandwich ELISA - Anti-RANTES antibody (ab9679)

Key features and details

Rabbit polyclonal to RANTES
Suitable for: Sandwich ELISA
Reacts with: Recombinant fragment
Isotype: unknown

产品名称

Anti-RANTES抗体
参阅全部 RANTES 一抗
描述

兔多克隆抗体to RANTES
宿主

Rabbit
经测试应用

适用于: Sandwich ELISAmore details
种属反应性

与反应: Recombinant fragment
免疫原

Recombinant full length protein corresponding to Human RANTES. Highly pure (>98%) recombinant hRANTES (human RANTES)
Database link: P13501
常规说明

Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

形式

Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
存储溶液

No preservative, sterile filtered
Concentration information loading...
纯度

Immunogen affinity purified
克隆

多克隆
同种型

unknown
轻链类型

unknown
研究领域
- Neuroscience
- Processes

Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

The Abpromise guarantee

Abpromise™承诺保证使用ab9679于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Sandwich ELISA		Use at an assay dependent concentration.

说明
Sandwich ELISA Use at an assay dependent concentration.

功能

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
组织特异性

T-cell and macrophage specific.
序列相似性

Belongs to the intercrine beta (chemokine CC) family.
翻译后修饰

N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
细胞定位

Secreted.
Target information above from: UniProt accession P13501 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
别名
- Beta chemokine RANTES antibody
- Beta chemokine RANTES precursor antibody
- C C motif chemokine 5 antibody
- CCL 5 antibody
- CCL5 antibody
- CCL5_HUMAN antibody
- Chemokine (C C motif) ligand 5 antibody
- Chemokine CC Motif Ligand 5 antibody
- D17S136E antibody
- EoCP antibody
- Eosinophil chemotactic cytokine antibody
- MGC17164 antibody
- RANTES(4-68) antibody
- Regulated upon activation normally T expressed and presumably secreted antibody
- SCYA 5 antibody
- SCYA5 antibody
- SIS delta antibody
- SIS-delta antibody
- SISd antibody
- Small inducible cytokine A5 (RANTES) antibody
- Small inducible cytokine A5 antibody
- Small inducible cytokine subfamily A (Cys Cys) member 5 antibody
- Small-inducible cytokine A5 antibody
- T cell specific protein p288 antibody
- T cell specific protein RANTES antibody
- T cell specific RANTES protein antibody
- T cell-specific protein P228 antibody
- T-cell-specific protein RANTES antibody
- TCP 228 antibody
- TCP228 antibody
see all

Sandwich ELISA - Anti-RANTES antibody (ab9679)

ab9679 detecting RANTES by sandwich ELISA, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hRANTES.

Western blot protocols

Click here to view the general protocols

Datasheet download

Download

发表研究结果有使用 ab9679？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab9679 被引用在 30 文献中.

Xu H et al. Cancer associated fibroblast-derived CCL5 promotes hepatocellular carcinoma metastasis through activating HIF1α/ZEB1 axis. Cell Death Dis 13:478 (2022). PubMed: 35589690
Greenberg J et al. Metastatic pancreatic neuroendocrine tumors feature elevated T cell infiltration. JCI Insight 7:N/A (2022). PubMed: 36301668
Dickhout A et al. Rapid Internalization and Nuclear Translocation of CCL5 and CXCL4 in Endothelial Cells. Int J Mol Sci 22:N/A (2021). PubMed: 34298951
Wang X et al. An Immune-Related Gene Prognostic Index for Triple-Negative Breast Cancer Integrates Multiple Aspects of Tumor-Immune Microenvironment. Cancers (Basel) 13:N/A (2021). PubMed: 34771505
Zhuang Y et al. Blocking the CCL5-CCR5 Axis Using Maraviroc Promotes M1 Polarization of Macrophages Cocultured with Irradiated Hepatoma Cells. J Hepatocell Carcinoma 8:599-611 (2021). PubMed: 34178876

View all Publications for this product

客户评价及客户问答

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1-10 of 12 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Tonsil)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA Buffer pH 9.0

Permeabilization

Yes - Commercial Wash Buffer with Tween

Specification

Tonsil

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Rudolf Jung

Verified customer

提交于 Jan 14 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Breast cancer)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA

Permeabilization

Specification

Breast cancer

Blocking step

PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 25 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Colon)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA

Permeabilization

Specification

Colon

Blocking step

PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 23 2018

Application

ELISA

Sample

Human Recombinant protein (recombinant human RANTES was used for calibration)

Specification

recombinant human RANTES was used for calibration

Blocking step

BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Type

Sandwich (Capture)

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Tatiana Karakasheva

Verified customer

提交于 Nov 03 2015

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

Thermo Scientific Starting Block T20 Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate pH6.0

Sample

Human Tissue sections (esophagus)

Specification

esophagus

Permeabilization

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Tatiana Karakasheva

Verified customer

提交于 Oct 24 2014

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (12% Bis-Tris)

Sample

Human Tissue lysate - whole (Skeletal Muscle (Gastrocnemius))

Specification

Skeletal Muscle (Gastrocnemius)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 31 2013

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Both Cit (pH6) and Tris (pH8) work well

Sample

Human Tissue sections (Skeletal Muscle (Gastrocnemius))

Specification

Skeletal Muscle (Gastrocnemius)

Permeabilization

Fixative

Methacarn

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 31 2013

Question

I am looking to purchase an Anti-RANTES antibody in order to look at RANTEs expression in human platelets. I looked at product ab10590 and ab9679. Would either of these for Immunfloresence, or would you recommend a different anti-RANTES antibody?

Abcam community

Verified customer

Asked on Feb 22 2013

Answer

The best choice from what we have is the goat polyclonal ab10590. Other antibodies we have that are raised against RANTES may also work for immunocytochemistry but they have not been tested in this application.

The exception is the rabbit monoclonal ab108933 but it has not been characterized as well as ab10590, and I am concerned that, since it is raised against the C-terminus, it might not be able to recognize platelet RANTES, depending on how RANTES is processed by platelets. The following note from the UniProt entry for the protein mentions that leukocytes secrete an N-terminal fragment.

"N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells."

http://www.uniprot.org/uniprot/P13501

I hope this helps. Please let us know if you have any other questions.

Abcam Scientific Support

回复于 Feb 22 2013

Question

human muscle samples, IHC-P
ab9666: staining everything including nucleus, AR: Tris, citrate, Ab: 5 ug/ml, block: goat serum, 2nd: OK, in WB sees lots of bands
ab9679: no staining, AR: Trs, citrate, Ab: 5 ug/ml o/n, 2nd: OK, block: goat serum

Abcam community

Verified customer

Asked on Dec 17 2012

Answer

free of charge repalcement given

Abcam Scientific Support

回复于 Dec 17 2012

Question

Antibody is in liquid form but on the datsheet we say that it is lyophilised and it needs to be reconstituted with 200µl of sterile water.

Abcam community

Verified customer

Asked on Dec 03 2012

Answer

Thank you for contacting us.
I have been in contact with the lab, I can confirm that ab9679 has already been reconstituted in 200ul sterile water. Since this antibody was lyophilised in PBS, this means that upon the addition of the water, the antibody has been re-dissolved in PBS. There is an error on the datasheet concerning the reconstitution in sterile water and we apologize for this and thank you for bringing this to our attention as it will allow us to update our datasheet.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Abcam Scientific Support

回复于 Dec 03 2012

1-10 of 12 Abreviews or Q&A

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Key features and details

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

同种型

轻链类型

研究领域

相关产品

Compatible Secondaries

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

翻译后修饰

细胞定位

别名

图片

实验方案

数据表及文件

Datasheet download

文献 (30)

客户评价及客户问答

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RANTES antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RANTES antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RANTES antibody

ELISA abreview for Anti-RANTES antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RANTES antibody

Western blot abreview for Anti-RANTES antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RANTES antibody