Anti-RANKL抗体[12A668] - BSA and Azide free (ab45039)
Key features and details
- Mouse monoclonal [12A668] to RANKL - BSA and Azide free
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-RANKL抗体[12A668] - BSA and Azide free
参阅全部 RANKL 一抗 -
描述
小鼠单克隆抗体[12A668] to RANKL - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Mouse RANKL aa 1-350.
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阳性对照
- IHC-P: Human lymph node and liver tissue. ICC/IF: HeLa (human epithelial cell line from cervix adenocarcinoma) cells. WB: Human lymph node lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
12A668 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45039于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
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IHC-P | (1) |
Use a concentration of 1 - 5 µg/ml.
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说明 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). |
IHC-P
Use a concentration of 1 - 5 µg/ml. |
靶标
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功能
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. -
组织特异性
Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. -
疾病相关
Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17. -
细胞定位
Cytoplasm; Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8600 Human
- Omim: 602642 Human
- SwissProt: O14788 Human
- Unigene: 333791 Human
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别名
- CD254 antibody
- hRANKL2 antibody
- ODF antibody
see all
图片
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Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039) at 2 µg + transfected cell lysate
Predicted band size: 35 kDa
Observed band size: 35 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)
Paraffin embedded human lymph node tissue stained for RANKL with ab45039 at 5 µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)
ICC/IF image of ab45039 stained HeLa cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45039, 10 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)
Paraffin embedded human liver tissue stained for RANKL with ab45039 at a 1/500 dilution. Peroxidase-conjugate and DAB chromogen. A 2 hour incubation at RT was used.
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Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039) at 0.5 µg/ml + human lymph node lysate in RIPA buffer at 35 µg
Predicted band size: 35 kDa
Additional bands at: 28 kDa (possible isoform)Band detected at ~35kDa and ~28kDa. (Expected MW of 35.5kDa according to NP_003692.1 and of 27.7kDa according to NP_143026.1)
实验方案
数据表及文件
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Datasheet download
文献 (74)
ab45039 被引用在 74 文献中.
- Ha P et al. Bisphosphonate conjugation enhances the bone-specificity of NELL-1-based systemic therapy for spaceflight-induced bone loss in mice. NPJ Microgravity 9:75 (2023). PubMed: 37723136
- Lv H et al. A static magnetic field improves bone quality and balances the function of bone cells with regulation on iron metabolism and redox status in type 1 diabetes. FASEB J 37:e22985 (2023). PubMed: 37249350
- Li Y et al. Melatonin promotes the restoration of bone defects via enhancement of miR-335-5p combined with inhibition of TNFα/NF-κB signaling. FASEB J 37:e22711 (2023). PubMed: 36520091
- Xu Y et al. Expression of spleen macrophages in a mouse model of alveolar bone resorption periodontitis. Cell Mol Biol (Noisy-le-grand) 69:125-131 (2023). PubMed: 37605579
- Ge Q et al. Ambient PM2.5 Exposure and Bone Homeostasis: Analysis of UK Biobank Data and Experimental Studies in Mice and in Vitro. Environ Health Perspect 131:107002 (2023). PubMed: 37792558