Anti-RanGAP1抗体(ab2081)
Key features and details
- Goat polyclonal to RanGAP1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-RanGAP1抗体
参阅全部 RanGAP1 一抗 -
描述
山羊多克隆抗体to RanGAP1 -
宿主
Goat -
特异性
There is a hypothetical protein called "similar to RANGAP1" (XP_139737.2) that is virtually identical. -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide: ASEDIAKLAETLAK, corresponding to N terminal amino acids 2-15 of Human RanGAP1.
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阳性对照
- 3T3 lysate.
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常规说明
Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP 1 and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP 1 is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody说明
Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP 1 and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP 1 is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 70 kDa.
A 1 hour primary incubation is recommended for this product. Detects a band of approximately 70 kDa in human brain lysates and 3T3 lysates (predicted MW of 68kDa according to NP_002874). |
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ICC/IF |
1/500.
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说明 |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 70 kDa. A 1 hour primary incubation is recommended for this product. Detects a band of approximately 70 kDa in human brain lysates and 3T3 lysates (predicted MW of 68kDa according to NP_002874). |
ICC/IF
1/500. |
靶标
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功能
GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. -
组织特异性
Highly expressed in brain, thymus and testis. -
序列相似性
Belongs to the RNA1 family.
Contains 6 LRR (leucine-rich) repeats. -
翻译后修饰
Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis.
Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9. -
细胞定位
Cytoplasm. Nucleus membrane. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation. - Information by UniProt
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数据库链接
- Entrez Gene: 5905 Human
- Entrez Gene: 19387 Mouse
- Omim: 602362 Human
- SwissProt: P46060 Human
- SwissProt: P46061 Mouse
- Unigene: 183800 Human
- Unigene: 270975 Mouse
- Unigene: 475271 Mouse
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别名
- Fug 1 antibody
- Fug1 antibody
- GTPase-activating protein, RAN, 1 antibody
see all
图片
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ab2081 at a concentration of 0.2
µ g/ml staining approximately 70kda RanGAP1 in 3T3 whole cell lysate (RIPA buffer, 35µ g total protein per lane) by Western blot (ECL). Primary incubation = 1 hour. ab2081 at a concentration of 0.2µg/ml staining approximately 70kda RanGAP1 in 3T3 whole cell lysate (RIPA buffer, 35µg total protein per lane) by Western blot (ECL). Primary incubation = 1 hour. -
Immunocytochemistry/ Immunofluorescence - Anti-RanGAP1 antibody (ab2081)Image courtesy of Marilena Ciciarello and Patrizia Lavia, University 'La Sapienza' CNR, Italyab2081 (1/500) detecting RanGap1 in mitotic and interphase Hela cells (green). Dapi was used as counterstain in order to highlight the nucleus (blue) and cells were fixed both in Methanol (-20 °C/ 6 mins, followed by 3 washes in PBS) or Paraformaldehyde (3,7% PFA /10 min at room temperature, 0,1% Triton/PBS 5 min, glycine 5 min, followed by 3 washes in PBS); This antibody also gave good results in NIH-3T3 cells under both the above mentioned fixation procedures (data not shown).
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab2081 被引用在 3 文献中.
- Zhang S et al. The ataxin-1 interactome reveals direct connection with multiple disrupted nuclear transport pathways. Nat Commun 11:3343 (2020). PubMed: 32620905
- Barbato S et al. Karyopherin enrichment at the nuclear pore complex attenuates Ran permeability. J Cell Sci 133:N/A (2020). PubMed: 31932502
- He X et al. Characterization of the loss of SUMO pathway function on cancer cells and tumor proliferation. PLoS One 10:e0123882 (2015). PubMed: 25860128