Anti-RanBP2抗体[mAbcam58385] (ab58385)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP2 antibody [mAbcam58385] (ab58385)](https://www.abcam.cn/ps/products/58/ab58385/Images/ab58385-195162-ab58385edited.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP2 antibody [mAbcam58385] (ab58385)")
Key features and details
- Mouse monoclonal [mAbcam58385] to RanBP2
- Suitable for: IHC-P, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-RanBP2抗体[mAbcam58385]
参阅全部 RanBP2 一抗 -
描述
小鼠单克隆抗体[mAbcam58385] to RanBP2 -
宿主
Mouse -
经测试应用
适用于: IHC-P, Flow Cyt (Intra)more details
不适用于: WB -
种属反应性
与反应: Human
预测可用于: Mouse, Cow
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 3100 - 3200 of Human RanBP2.
参阅Abcam的专有抗源政策 (Peptide available as ab59186.) -
阳性对照
- IHC-P: Human colon adenocarcinoma tissue. Flow Cyt (Intra): HeLa cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 6.97% L-Arginine, PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
mAbcam58385 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58385于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Flow Cyt (Intra) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
Flow Cyt (Intra)
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Could also have isomerase or chaperone activity and may bind RNA or DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1. -
通路
Protein modification; protein sumoylation. -
疾病相关
Defects in RANBP2 are the cause of susceptibility to encephalopathy acute necrotizing type 1 (ANE1) [MIM:608033]. A rapidly progressive encephalopathy manifesting in susceptibile individuals with seizures and coma. It can occur within days in otherwise healthy children after common viral infections such as influenza and parainfluenza, without evidence of viral infection of the brain or inflammatory cell infiltration. Brain T2-weighted magnetic resonance imaging reveals characteristic symmetric lesions present in the thalami, pons and brainstem. -
序列相似性
Contains 1 PPIase cyclophilin-type domain.
Contains 4 RanBD1 domains.
Contains 8 RanBP2-type zinc fingers.
Contains 1 TPR repeat. -
结构域
Contains F-X-F-G repeats. -
翻译后修饰
Polyubiquitinated by PARK2, which leads to proteasomal degradation. -
细胞定位
Nucleus > nuclear pore complex. Cytoplasmic filaments. - Information by UniProt
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数据库链接
- Entrez Gene: 785768 Cow
- Entrez Gene: 5903 Human
- Entrez Gene: 19386 Mouse
- Omim: 601181 Human
- SwissProt: P48820 Cow
- SwissProt: P49792 Human
- SwissProt: Q8C9K9 Mouse
- SwissProt: Q9ERU9 Mouse
see all -
别名
- 358 kDa nucleoporin antibody
- ANE1 antibody
- E3 SUMO-protein ligase RanBP2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP2 antibody [mAbcam58385] (ab58385)
IHC image of RanBP2 staining in Human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58385, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Flow Cytometry (Intracellular) - Anti-RanBP2 antibody [mAbcam58385] (ab58385)](https://www.abcam.cn/ps/products/58/ab58385/Images/RanBP2-Primary-antibodies-ab58385-4.jpg)
Flow Cytometry (Intracellular) - Anti-RanBP2 antibody [mAbcam58385] (ab58385)Overlay histogram showing HeLa cells stained with ab58385 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58385, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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