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Cell Biology Cell Cycle Cell Division Spindle

Anti-RanBP2抗体(ab2938)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (9)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP2 antibody (ab2938)
  • Western blot - Anti-RanBP2 antibody (ab2938)

Key features and details

  • Rabbit polyclonal to RanBP2
  • Suitable for: IHC-P, WB
  • Reacts with: Cow, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-RanBP2抗体
    参阅全部 RanBP2 一抗
  • 描述

    兔多克隆抗体to RanBP2
  • 宿主

    Rabbit
  • 特异性

    Detects Ran binding protein 2 (RanBP 2) from human and bovine retina.
  • 经测试应用

    适用于: IHC-P, WBmore details
  • 种属反应性

    与反应: Cow, Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to Human RanBP2 aa 1-18.
    Sequence:

    MRRSKADVERYIASVQGS


    (Peptide available as ab4939)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • IHC-P: Bovine retinal extracts. WB: Human retinal extracts.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Sumo
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Ubiquitin like Modifiers

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • RanBP2 peptide (ab4939)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2938于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use a concentration of 10 µg/ml.

Immunohistochemical staining of bovine retina results in cytoplasmic staining of cells within the inner nuclear layer as well as the ganglion cells.

WB
Use a concentration of 1 µg/ml. Detects a band of approximately 360 kDa (predicted molecular weight: 358 kDa).Can be blocked with RanBP2 peptide (ab4939).

Detects a band of approximately 360 kDa representing RanBP 2 from bovine retinal extracts.

说明
IHC-P
Use a concentration of 10 µg/ml.

Immunohistochemical staining of bovine retina results in cytoplasmic staining of cells within the inner nuclear layer as well as the ganglion cells.

WB
Use a concentration of 1 µg/ml. Detects a band of approximately 360 kDa (predicted molecular weight: 358 kDa).Can be blocked with RanBP2 peptide (ab4939).

Detects a band of approximately 360 kDa representing RanBP 2 from bovine retinal extracts.

靶标

  • 功能

    E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Could also have isomerase or chaperone activity and may bind RNA or DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1.
  • 通路

    Protein modification; protein sumoylation.
  • 疾病相关

    Defects in RANBP2 are the cause of susceptibility to encephalopathy acute necrotizing type 1 (ANE1) [MIM:608033]. A rapidly progressive encephalopathy manifesting in susceptibile individuals with seizures and coma. It can occur within days in otherwise healthy children after common viral infections such as influenza and parainfluenza, without evidence of viral infection of the brain or inflammatory cell infiltration. Brain T2-weighted magnetic resonance imaging reveals characteristic symmetric lesions present in the thalami, pons and brainstem.
  • 序列相似性

    Contains 1 PPIase cyclophilin-type domain.
    Contains 4 RanBD1 domains.
    Contains 8 RanBP2-type zinc fingers.
    Contains 1 TPR repeat.
  • 结构域

    Contains F-X-F-G repeats.
  • 翻译后修饰

    Polyubiquitinated by PARK2, which leads to proteasomal degradation.
  • 细胞定位

    Nucleus > nuclear pore complex. Cytoplasmic filaments.
  • Target information above from: UniProt accession P49792 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 735654 Chimpanzee
    • Entrez Gene: 785768 Cow
    • Entrez Gene: 5903 Human
    • Omim: 601181 Human
    • SwissProt: H2QII6 Chimpanzee
    • SwissProt: P48820 Cow
    • SwissProt: P49792 Human
    • Unigene: 199561 Human
    • Unigene: 715056 Human
    see all
  • 别名

    • 358 kDa nucleoporin antibody
    • ANE1 antibody
    • E3 SUMO-protein ligase RanBP2 antibody
    • IIAE3 antibody
    • Nuclear pore complex protein Nup358 antibody
    • Nucleoporin 358 antibody
    • Nucleoporin Nup358 antibody
    • NUP358 antibody
    • p270 antibody
    • RAN binding protein 2 antibody
    • Ran-binding protein 2 antibody
    • RANBP2 antibody
    • RBP2_HUMAN antibody
    • Transformation related protein 2 antibody
    • TRP 1 antibody
    • TRP 2 antibody
    • TRP1 antibody
    • TRP2 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP2 antibody (ab2938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP2 antibody (ab2938)

    Immunohistochemical staining of RanBP2 in bovine retina using ab2938.

  • Western blot - Anti-RanBP2 antibody (ab2938)
    Western blot - Anti-RanBP2 antibody (ab2938)

    Western blot of RanBP2 from human retinal extracts using ab2938. 

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (9)

发表研究结果有使用 ab2938?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2938 被引用在 9 文献中.

  • Li H  et al. Nucleolar and spindle associated protein 1 promotes metastasis of cervical carcinoma cells by activating Wnt/ß-catenin signaling. J Exp Clin Cancer Res 38:33 (2019). PubMed: 30678687
  • Saito A  et al. Roles of Capsid-Interacting Host Factors in Multimodal Inhibition of HIV-1 by PF74. J Virol 90:5808-23 (2016). PubMed: 27076642
  • Henning MS  et al. In vivo functions of CPSF6 for HIV-1 as revealed by HIV-1 capsid evolution in HLA-B27-positive subjects. PLoS Pathog 10:e1003868 (2014). WB ; Human . PubMed: 24415937
  • Ocwieja KE  et al. HIV integration targeting: a pathway involving Transportin-3 and the nuclear pore protein RanBP2. PLoS Pathog 7:e1001313 (2011). WB ; Human . PubMed: 21423673
  • Nagai M  et al. Mice lacking Ran binding protein 1 are viable and show male infertility. FEBS Lett 585:791-6 (2011). IHC ; Mouse . PubMed: 21310149
  • Zhang R  et al. Perturbation of host nuclear membrane component RanBP2 impairs the nuclear import of human immunodeficiency virus -1 preintegration complex (DNA). PLoS One 5:e15620 (2010). WB ; Human . PubMed: 21179483
  • Felix RS  et al. SAGE analysis highlights the importance of p53csv, ddx5, mapkapk2 and ranbp2 to multiple myeloma tumorigenesis. Cancer Lett 278:41-8 (2009). IHC-P ; Human . PubMed: 19171422
  • Shitashige M  et al. Regulation of Wnt signaling by the nuclear pore complex. Gastroenterology 134:1961-71, 1971.e1-4 (2008). PubMed: 18439914
  • Jain P  et al. Identification of human T cell leukemia virus type 1 tax amino acid signals and cellular factors involved in secretion of the viral oncoprotein. J Biol Chem 282:34581-93 (2007). Dot blot . PubMed: 17897946

客户评价及客户问答

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Question

I have just recieved the antibody RanBP2 antibody. In the datasheet (and on your homepage), you show an immunohistochemical staining with this antibody. I would like to know what kind of antigen retrieval that is used with this antibody, when performing immunohistochemistry ?

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Abcam community

Verified customer

Asked on Apr 02 2004

Answer

This was done on cryosections fixed in 2% paraformaldehyde and permeabilized with Triton X-100 (or saponin can be used).

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Abcam Scientific Support

回复于 Apr 05 2004

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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