Anti-RanBP1抗体(ab17034)
Key features and details
- Goat polyclonal to RanBP1
- Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RanBP1抗体
参阅全部 RanBP1 一抗 -
描述
山羊多克隆抗体to RanBP1 -
宿主
Goat -
经测试应用
适用于: Flow Cyt, ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human RanBP1 aa 150 to the C-terminus.
Database link: P43487 -
阳性对照
- WB: Human duodenum lysate. A431, HeLa and MCF7 whole cell lysate. ICC/IF: HEK293 and HeLa cells. Flow Cyt: HEK293 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17034于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use a concentration of 10 µg/ml.
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| ICC/IF |
Use a concentration of 10 µg/ml.
|
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| WB |
Use a concentration of 0.05 - 0.1 µg/ml. Detects a band of approximately 28-30 kDa (predicted molecular weight: 23 kDa).
A 1 hour primary incubation is recommended for this product. |
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| IHC-P |
Use a concentration of 3 - 5 µg/ml.
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| 说明 |
|---|
|
Flow Cyt
Use a concentration of 10 µg/ml. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 0.05 - 0.1 µg/ml. Detects a band of approximately 28-30 kDa (predicted molecular weight: 23 kDa). A 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 3 - 5 µg/ml. |
靶标
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功能
Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane. -
序列相似性
Belongs to the RANBP1 family.
Contains 1 RanBD1 domain. - Information by UniProt
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数据库链接
- Entrez Gene: 5902 Human
- Omim: 601180 Human
- SwissProt: P43487 Human
- Unigene: 24763 Human
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别名
- HTF9A antibody
- RAN binding protein 1 antibody
- Ran specific GTPase activating protein antibody
see all
图片
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Flow Cytometry - Anti-RanBP1 antibody (ab17034)Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Western blot - Anti-RanBP1 antibody (ab17034)Anti-RanBP1 antibody (ab17034) at 0.3 µg/ml + Human duodenum lysate (RIPA buffer) at 35 µg
Predicted band size: 23 kDaDetected by chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (ab17034)Immunofluorescence analysis of paraformaldehyde fixed HEK-293 (Human epithelial cell line from embryonic kidney) cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/ml) followed by Alexa Fluor 488® secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue).
Negative control: Non-immunized goat IgG (10 µg/ml) followed by Alexa Fluor 488® secondary antibody (2 µg/ml).
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Western blot - Anti-RanBP1 antibody (ab17034)Lane 1 : Anti-RanBP1 antibody (ab17034) at 0.03 µg/ml
Lane 2 : Anti-RanBP1 antibody (ab17034) at 0.5 µg/ml
Lane 3 : Anti-RanBP1 antibody (ab17034) at 0.1 µg/ml
Lane 1 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 23 kDaDetected by chemiluminescence.
Lysates in RIPA buffer.
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Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (ab17034)Immunofluorescence analysis of paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/ml) followed by Alexa Fluor 488® secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue).
Negative control: Non-immunized goat IgG (10 µg/ml) followed by Alexa Fluor 488® secondary antibody (2 µg/ml).
数据表及文件
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Datasheet download
文献 (1)
ab17034 被引用在 1 文献中.
- Ogawa Y & Imamoto N Methods to separate nuclear soluble fractions reflecting localizations in living cells. iScience 24:103503 (2021). PubMed: 34934922
