AB17034

Anti-RanBP1抗体

Name: RanBP1抗体 (ab17034)| Abcam中文官网
Brand: Abcam Limited
SKU: AB17034
Price: 4606 CNY
Availability: InStock

Anti-RanBP1 antibody

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(2 Publications)

Goat Polyclonal RanBP1 antibody. Suitable for ICC/IF, WB, IHC-P, Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RANBP1 aa 150 to C-terminus.

查看别名

Ran-specific GTPase-activating protein, Ran-binding protein 1, RanBP1, RANBP1

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (AB17034)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (AB17034)

Immunofluorescence analysis of paraformaldehyde fixed HEK-293 (Human epithelial cell line from embryonic kidney) cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488^® secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue).

Negative control : Non-immunized goat IgG (10 μg/ml) followed by Alexa Fluor 488^® secondary antibody (2 μg/ml).

Flow Cytometry - Anti-RanBP1 antibody (AB17034)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-RanBP1 antibody (AB17034)

Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (AB17034)

Immunofluorescence analysis of paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488^® secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue).

Negative control : Non-immunized goat IgG (10 μg/ml) followed by Alexa Fluor 488^® secondary antibody (2 μg/ml).

Supplier Data

Western blot - Anti-RanBP1 antibody (AB17034)

Detected by chemiluminescence.

Lysates in RIPA buffer.

Lane 1:

Western blot - Anti-RanBP1 antibody (ab17034) at 0.03 µg/mL

Lane 2:

Western blot - Anti-RanBP1 antibody (ab17034) at 0.5 µg/mL

Lane 3:

Western blot - Anti-RanBP1 antibody (ab17034) at 0.1 µg/mL

Lane 1:

A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 35 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 35 µg

Predicted band size: 23 kDa

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Supplier Data

Western blot - Anti-RanBP1 antibody (AB17034)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-RanBP1 antibody (ab17034) at 0.3 µg/mL

All lanes:

Human duodenum lysate (RIPA buffer) at 35 µg

Predicted band size: 23 kDa

false

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, Flow Cyt, WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human RANBP1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P43487

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.05-0.1 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3-5 µg/mL", "IHCP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RanBP1 also known as Ran-binding protein 1 is an important player in the regulation of the Ran GTPase cycle integral to transport processes across the nuclear envelope. This protein has a mass of about 23 kDa. In humans RanBP1 shows expression in various tissues with higher levels observed in testis and brain. It directly interacts with Ran a small GTPase helping to modulate its activity by promoting the conversion between the active GTP-bound form and the inactive GDP-bound form.

Biological function summary

RanBP1 functions closely with Ran GTPase in the nucleocytoplasmic transport system where it is part of a complex with Ran and other factors like importins and exportins. It aids in the stabilization of the Ran-GTPase complex facilitating the release of cargo molecules and ensuring efficient transport cycles within the cells. RanBP1 in conjunction with Ran regulates the dynamic transport of proteins and RNA essential for maintaining cellular compartmentalization.

Pathways

The RanBP1 protein plays a role in the Ran GTPase cycle and nucleocytoplasmic transport pathways. These pathways are essential to cellular dynamics affecting overall transport between the cytoplasm and the nucleus. Furthermore RanBP1 affects interactions with proteins such as RanGAP1 contributing to the regulation of Ran activity cycles and supporting cellular functions including mitosis and RNA processing.

Research indicates that alterations in RanBP1 expression and function relate to cancer progression and neurodegenerative conditions. Misregulation of RanBP1 can affect nuclear transport balance impacting cancer-promoting pathways. Its interaction with Ran and its pathway-related proteins like importin beta suggests a role in tumorigenesis by contributing to aberrant cell cycle regulation and mitotic errors while its importance in neuron function suggests involvement in neurodegenerative disease mechanisms.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a role in RAN-dependent nucleocytoplasmic transport. Alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus (By similarity). Induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PubMed : 20485264). Promotes the disassembly of the complex formed by RAN and importin beta. Promotes dissociation of RAN from a complex with KPNA2 and CSE1L (By similarity). Required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (PubMed : 17671426). Does not increase the RAN GTPase activity by itself, but increases GTP hydrolysis mediated by RANGAP1 (PubMed : 7882974). Inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (PubMed : 7616957, PubMed : 7882974).

See full target information RANBP1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 24:103503 PubMed34934922

2021

Methods to separate nuclear soluble fractions reflecting localizations in living cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yutaka Ogawa,Naoko Imamoto

International journal of nanomedicine 15:6019-6032 PubMed32848399

2020

Ag/Au Bimetallic Nanoparticles Inhibit Tumor Growth and Prevent Metastasis in a Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Hector Katifelis,Iuliia Mukha,Penelope Bouziotis,Nadiia Vityuk,Charalampos Tsoukalas,Andreas C Lazaris,Anna Lyberopoulou,George E Theodoropoulos,Efstathios P Efstathopoulos,Maria Gazouli

View all publications

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Anti-RanBP1抗体

Anti-RanBP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (AB17034)

Flow Cytometry - Anti-RanBP1 antibody (AB17034)

Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (AB17034)

Western blot - Anti-RanBP1 antibody (AB17034)

Western blot - Anti-RanBP1 antibody (AB17034)