Anti-Ran抗体(ab53775)
Key features and details
- Rabbit polyclonal to Ran
- Suitable for: ELISA, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Ran抗体
参阅全部 Ran 一抗 -
描述
兔多克隆抗体to Ran -
宿主
Rabbit -
经测试应用
适用于: ELISA, ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide derived from Human Ran
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53775于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 23308199
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| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 23308199 |
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WB
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases. -
组织特异性
Expressed in a variety of tissues. -
序列相似性
Belongs to the small GTPase superfamily. Ran family. -
翻译后修饰
The N-terminus is blocked. -
细胞定位
Nucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 5901 Human
- Entrez Gene: 100045999 Mouse
- Entrez Gene: 19384 Mouse
- Entrez Gene: 84509 Rat
- Omim: 601179 Human
- SwissProt: P62826 Human
- SwissProt: P62827 Mouse
- SwissProt: P62828 Rat
see all -
别名
- Androgen receptor associated protein 24 antibody
- Androgen receptor-associated protein 24 antibody
- ARA 24 antibody
see all
图片
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Western blot - Anti-Ran antibody (ab53775)All lanes : Anti-Ran antibody (ab53775) at 1/500 dilution
Lane 1 : Extracts from LOVO cells
Lane 2 : Extracts from LOVO cells, plus immunising peptide
Predicted band size: 26 kDa
Observed band size: 26 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody (ab53775)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53775 at a 1/50 dilution.Left image un-treated.
Right image treated with immunizing peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab53775 被引用在 9 文献中.
- Yoo TY & Mitchison TJ O-GlcNAc modification of nuclear pore complexes accelerates bidirectional transport. J Cell Biol 220:N/A (2021). PubMed: 33909044
- Feng H et al. MiR-802 Suppresses Colorectal Cancer Cell Viability, Migration and Invasion by Targeting RAN. Cancer Manag Res 12:2291-2300 (2020). PubMed: 32273766
- Zhong Y et al. Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression. Int J Biol Sci 16:1941-1953 (2020). PubMed: 32398961
- Connell M et al. HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development. Elife 6:N/A (2017). PubMed: 28994651
- Khosravi B et al. Cytoplasmic poly-GA aggregates impair nuclear import of TDP-43 in C9orf72 ALS/FTLD. Hum Mol Genet 26:790-800 (2017). PubMed: 28040728
- Mastroeni D et al. Reduced RAN expression and disrupted transport between cytoplasm and nucleus; a key event in Alzheimer's disease pathophysiology. PLoS One 8:e53349 (2013). WB, IHC-P, ICC/IF ; Human . PubMed: 23308199
- Lecompte S et al. Genetic and molecular insights into the role of PROX1 in glucose metabolism. Diabetes 62:1738-45 (2013). PubMed: 23274905
- Amengual J et al. A mitochondrial enzyme degrades carotenoids and protects against oxidative stress. FASEB J 25:948-59 (2011). WB ; Mouse . PubMed: 21106934
- Lobo GP et al. {beta},{beta}-Carotene Decreases Peroxisome Proliferator Receptor {gamma} Activity and Reduces Lipid Storage Capacity of Adipocytes in a {beta},{beta}-Carotene Oxygenase 1-dependent Manner. J Biol Chem 285:27891-9 (2010). WB ; Mouse . PubMed: 20573961