Anti-RALB抗体(ab223479)
Key features and details
- Rabbit polyclonal to RALB
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-RALB抗体
参阅全部 RALB 一抗 -
描述
兔多克隆抗体to RALB -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cynomolgus monkey, Orangutan
-
免疫原
Recombinant full length protein corresponding to Human RALB aa 1 to the C-terminus.
Database link: P11234 -
阳性对照
- WB: Mouse brain and lung lysates; MCF7, HepG2, Jurkat, NIH/3T3 and HeLa whole cell lysates. IHC-P: Human ovarian cancer tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
- HeLa whole cell lysate (ab150035)
- Hep G2 whole cell lysate (ab166833)
- Mouse lung normal tissue lysate - total protein (ab29297)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (ab4022)
- NIH/3T3 whole cell lysate (ab7179)
- Jurkat whole cell lysate (ab7899)
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223479于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
|
|
| IHC-P |
1/20 - 1/200.
|
| 说明 |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
|
IHC-P
1/20 - 1/200. |
靶标
-
功能
Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Required both to stabilize the assembly of the exocyst complex and to localize functional exocyst complexes to the leading edge of migrating cells. Plays a role in the late stages of cytokinesis and is required for the abscission of the bridge joining the sister cells emerging from mitosis. Required for suppression of apoptosis. -
序列相似性
Belongs to the small GTPase superfamily. Ras family. -
翻译后修饰
Prenylation is essential for membrane localization. The geranylgeranylated form and the farnesylated mutant does not undergo alternative prenylation in response to geranylgeranyltransferase I inhibitors (GGTIs) and farnesyltransferase I inhibitors (FTIs). -
细胞定位
Cell membrane. During late cytokinesis localizes at the midbody. - Information by UniProt
-
数据库链接
- Entrez Gene: 101925782 Cynomolgus monkey
- Entrez Gene: 5899 Human
- Entrez Gene: 64143 Mouse
- Entrez Gene: 100174018 Orangutan
- Entrez Gene: 116546 Rat
- Omim: 179551 Human
- SwissProt: Q4R379 Cynomolgus monkey
- SwissProt: P11234 Human
see all -
别名
- 5730472O18Rik antibody
- dRalb antibody
- GTP binding protein antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALB antibody (ab223479)
Paraffin-embedded human ovarian cancer tissue stained for RALB using ab223479 at 1/100 dilution in immunohistochemical analysis.
-
Western blot - Anti-RALB antibody (ab223479)All lanes : Anti-RALB antibody (ab223479) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse lung lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lane 7 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab223479 被引用在 3 文献中.
- Wang Y et al. MicroRNA-139 inhibits pancreatic-cancer carcinogenesis by suppressing RalB via the Ral/RAC/PI3K pathway. Arch Biochem Biophys 704:108719 (2021). PubMed: 33290747
- Thies KA et al. The small G-protein RalA promotes progression and metastasis of triple-negative breast cancer. Breast Cancer Res 23:65 (2021). PubMed: 34118960
- Cruz VH et al. Axl-mediated activation of TBK1 drives epithelial plasticity in pancreatic cancer. JCI Insight 5:N/A (2019). PubMed: 30938713