AB241499

Anti-RAI14抗体

Name: RAI14抗体 (ab241499)| Abcam中文官网
Brand: Abcam Limited
SKU: AB241499
Price: 4864 CNY
Availability: InStock

Anti-RAI14 antibody

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(2 Publications)

Rabbit Polyclonal RAI14 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RAI14 aa 1-50.

查看别名

KIAA1334, NORPEG, RAI14, Ankycorbin, Ankyrin repeat and coiled-coil structure-containing protein, Novel retinal pigment epithelial cell protein, Retinoic acid-induced protein 14

2 Images

Supplier Data

Immunoprecipitation - Anti-RAI14 antibody (AB241499)

RAI14 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241499 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241499 at 1 μg/ml.

Lane 1 : ab241499 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-RAI14 antibody (ab241499)

Predicted band size: 110 kDa

false

Supplier Data

Western blot - Anti-RAI14 antibody (AB241499)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-RAI14 antibody (ab241499) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 50 µg

Predicted band size: 110 kDa

true

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human RAI14 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9P0K7

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Antibody was affinity purified using an epitope specific to RAI14 immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

RAI14 also known as Ankycorbin or Norpeg is a protein that plays a role in cytoskeletal organization and cellular architecture. The protein has a molecular mass of approximately 110 kDa. Researchers have found RAI14 to be expressed in several tissues including testis heart and retina. In these tissues RAI14 functions to maintain cell structure and stability by interacting with actin filaments which are important components of the cell cytoskeleton. This interaction suggests RAI14’s involvement in regulating actin dynamics.

Biological function summary

Researchers believe RAI14 contributes to cellular processes by participating in complex formation with other proteins like actin. It modulates the arrangement of the actin cytoskeleton which affects cell shape movement and mechanical stability. The involvement of RAI14 in actin dynamics indicates its role in providing cellular elasticity and adaptability important for cells undergoing morphological changes. Studies also suggest that RAI14 might influence processes like cell adhesion which is key for maintaining tissue integrity.

Pathways

Scientists link RAI14 to actin-related pathways and cellular morphogenesis. It works alongside proteins such as filamin A and vinculin that interact with the actin cytoskeleton to manage cell shape and mechanical responses. RAI14’s function in these pathways is important for understanding how cells migrate and how they respond to mechanical stress. These processes are important for tissue development and repair highlighting the significance of RAI14 in maintaining cellular homeostasis.

Scientists have associated RAI14 with conditions affecting cell structure and motility. For instance RAI14 dysregulation has been observed in certain types of cancer where changes in cell adhesion and migration contribute to tumor progression. Additionally abnormalities in the expression or function of actin-related proteins like RAI14 can also lead to retinal diseases due to its expression in retinal tissue. These connections highlight the importance of RAI14 in understanding and potentially targeting cytoskeletal disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a role in actin regulation at the ectoplasmic specialization, a type of cell junction specific to testis. Important for establishment of sperm polarity and normal spermatid adhesion. May also promote integrity of Sertoli cell tight junctions at the blood-testis barrier.

See full target information RAI14

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1613458 PubMed40823069

2025

Polyamine metabolism related gene index prediction of prognosis and immunotherapy response in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoya Wang,Shouliang Cai,Qing Gao,Yidong Chen,Xue Han,Fangjian Shang,Chunyan Liang,Guolian Zhu,Bo Chen

International journal of molecular sciences 23: PubMed36233342

2022

RAI14 Promotes Melanoma Progression by Regulating the FBXO32/c-MYC Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Xu,Pengfei Shi,Fanwei Xia,Xuan Zhao,Junfan Chen,Rui Geng,Hongjuan Cui,Liqun Yang

View all publications

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Anti-RAI14抗体

Anti-RAI14 antibody

Immunoprecipitation - Anti-RAI14 antibody (AB241499)

Western blot - Anti-RAI14 antibody (AB241499)