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重组RabMAb

重组Anti-RAGE抗体[EPR16849-75] - Low endotoxin，Azide free (ab225533)

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Immunoprecipitation - Anti-RAGE antibody [EPR16849-75] - Low endotoxin, Azide free (ab225533)
  • Anti-RAGE antibody [EPR16849-75] - Low endotoxin, Azide free (ab225533)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16849-75] to RAGE - Low endotoxin, Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat

概述

  • 产品名称

    Anti-RAGE抗体[EPR16849-75] - Low endotoxin，Azide free
    参阅全部 RAGE 一抗

  • 描述

    兔单克隆抗体[EPR16849-75] to RAGE - Low endotoxin，Azide free

  • 宿主

    Rabbit

  • 特异性

    RAGE is typically expressed at low levels under normal physiological conditions in majority of tissues except normal lung tissue. When testing other tissues, please use lung tissue as a positive control.

  • 经测试应用

    适用于: IP, WBmore details

  • 种属反应性

    与反应: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Mouse and rat lung lysates. IP: Mouse lung whole cell lysate.

  • 常规说明

    ab225533 is the carrier-free version of ab181293.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab225533于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
说明
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

靶标

  • 功能

    Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.

  • 组织特异性

    Endothelial cells.

  • 序列相似性

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 细胞定位

    Secreted and Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Advanced glycosylation end product-specific receptor antibody
      • Ager antibody
      • DAMA 358M23.4 antibody
      • MGC2235 antibody
      • MGC22357 antibody
      • RAGE_HUMAN antibody
      • Receptor for advanced glycation end products antibody
      • Receptor for advanced glycosylation end products antibody
      see all

    图片

    • Immunoprecipitation - Anti-RAGE antibody [EPR16849-75] - Low endotoxin, Azide free (ab225533)
      Immunoprecipitation - Anti-RAGE antibody [EPR16849-75] - Low endotoxin, Azide free (ab225533)

      This IP data was generated using the same anti-RAGE antibody clone [EPR16849-75] in a different buffer formulation (cat# ab181293).

      RAGE was immunoprecipitated from 1 mg of mouse lung whole cell lysate with ab181293 at 1/50 dilution.

      Western blot was performed from the immunoprecipitate using ab181293 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: Mouse lung whole cell lysate 10µg (Input).

      Lane 2: ab181293 IP in mouse lung whole cell lysate.

      Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab181293 in mouse lung whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

    • Anti-RAGE antibody [EPR16849-75] - Low endotoxin, Azide free (ab225533)
      Anti-RAGE antibody [EPR16849-75] - Low endotoxin, Azide free (ab225533)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (1)

    发表研究结果有使用 ab225533？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab225533 被引用在 1 文献中.

    • Lee CY  et al. Novel Therapeutic Effects of Pterosin B on Ang II-Induced Cardiomyocyte Hypertrophy. Molecules 25:N/A (2020). PubMed: 33198253

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