重组Anti-RAGE抗体[EPR12206] (ab172473)
![Western blot - Anti-RAGE antibody [EPR12206] (ab172473)](https://www.abcam.cn/ps/products/172/ab172473/Images/ab172473-341535-172473.jpg "Western blot - Anti-RAGE antibody [EPR12206] (ab172473)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12206] to RAGE
- Suitable for: WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
概述
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产品名称
Anti-RAGE抗体[EPR12206]
参阅全部 RAGE 一抗 -
描述
兔单克隆抗体[EPR12206] to RAGE -
宿主
Rabbit -
特异性
Recent tests in our laboratory showed that the antibody detects the band of interest in tissue lysates, but it did not detect the protein in cell lysate.
RAGE is typically expressed at low levels under normal physiological conditions in majority of tissues except normal lung tissue. When testing other tissues, please use lung tissue as a positive control.
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经测试应用
适用于: WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat
不与反应: Human -
免疫原
Synthetic peptide within Human RAGE aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q15109 -
阳性对照
- Mouse lung, Rat lung.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12206 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab172473于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
We recommend ab181369 for human samples. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. We recommend ab181369 for human samples. |
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.
靶标
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功能
Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. -
组织特异性
Endothelial cells. -
序列相似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 11596 Mouse
- Entrez Gene: 81722 Rat
- SwissProt: Q62151 Mouse
- SwissProt: Q63495 Rat
- Unigene: 3383 Mouse
- Unigene: 9829 Rat
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别名
- Advanced glycosylation end product-specific receptor antibody
- Ager antibody
- DAMA 358M23.4 antibody
see all
图片
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Western blot - Anti-RAGE antibody [EPR12206] (ab172473)All lanes : Anti-RAGE antibody [EPR12206] (ab172473) at 1/1000 dilution
Lane 1 : Mouse lung lysates
Lane 2 : Mouse brain lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse heart lysates
Lane 5 : Mouse liver lysates
Lane 6 : Mouse spleen lysates
Lane 7 : Rat lung lysates
Lane 8 : Rat brain lysates with 5% NFDM/TBST
Lane 9 : Rat kidney lysates
Lane 10 : Rat heart lysates
Lane 11 : Rat spleen lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time:
Lane 1 to 6: 3 second
Lane 7 to 11: 20 secondsThe expression profile and molecular mass observed is consistent with what has been described in the literature (PMID: 16315007; 18355449; 18245812)
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![Anti-RAGE antibody [EPR12206] (ab172473)](https://www.abcam.cn/ps/products/172/ab172473/Images/ab172473-3-benefits-of-recombinant-antibodies.png)
Anti-RAGE antibody [EPR12206] (ab172473)
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab172473 被引用在 6 文献中.
- Chen R et al. HMGB1 as a potential biomarker and therapeutic target for severe COVID-19. Heliyon 6:e05672 (2020). PubMed: 33313438
- Tsitsilashvili E et al. Myelin basic protein charge isomers change macrophage polarization. J Inflamm Res 12:25-33 (2019). PubMed: 30774410
- Ryan D et al. Targeting AXL and RAGE to prevent geminin overexpression-induced triple-negative breast cancer metastasis. Sci Rep 9:19150 (2019). PubMed: 31844158
- Ananthula S et al. Geminin overexpression-dependent recruitment and crosstalk with mesenchymal stem cells enhance aggressiveness in triple negative breast cancers. Oncotarget 7:20869-89 (2016). PubMed: 26989079
- Gu JJ et al. Recombinant human soluble thrombomodulin protects against brain injury in a CVST rat model, via downregulation of the HMGB1-RAGE axis. Mol Med Rep 14:5217-5222 (2016). PubMed: 27840921
- Wang XW et al. Small interfering RNA targeting receptor for advanced glycation end products suppresses the generation of proinflammatory cytokines. Exp Ther Med 10:584-590 (2015). PubMed: 26622358