Anti-Rag A抗体(ab128196)
Key features and details
- Rabbit polyclonal to Rag A
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Rag A抗体 -
描述
兔多克隆抗体to Rag A -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Mammals
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免疫原
Recombinant full length protein corresponding to Human Rag A.
Database link: Q7L523 -
阳性对照
- HeLa cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 6
Preservative: 0.05% Sodium azide
Constituent: 99% Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128196于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/2000. Predicted molecular weight: 37 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
WB
1/1000 - 1/2000. Predicted molecular weight: 37 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death. -
组织特异性
Ubiquitously expressed with highest levels of expression in skeletal muscle, heart, and brain. -
序列相似性
Belongs to the GTR/RAG GTP-binding protein family. -
细胞定位
Cytoplasm. Nucleus. Lysosome. Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state. Colocalizes in vivo with adenovirus E3-14.7K mainly to the cytoplasm especially near the nuclear membrane and in discrete foci on or near the plasma membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10670 Human
- Entrez Gene: 68441 Mouse
- Omim: 612194 Human
- SwissProt: Q7L523 Human
- SwissProt: Q80X95 Mouse
- Unigene: 702275 Human
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别名
- Adenovirus E3 14.7 kDa-interacting protein 1 antibody
- FIP-1 antibody
- Rag A antibody
see all
图片
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Western blot - Anti-Rag A antibody (ab128196)All lanes : Anti-Rag A antibody (ab128196) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : MOLT4 cell lysate
Lane 4 : NIH-3T3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 37 kDa -
Western blot - Anti-Rag A antibody (ab128196)Anti-Rag A antibody (ab128196) at 1/1000 dilution + HeLa whole cell lysate at 10 µg
Predicted band size: 37 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Rag A antibody (ab128196)HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% TritonX 100 and reacted with anti-Rag A antibody at 1/100 dilution. As the 2nd antibody, anti-rabbit IgG antibody conjugated with Alexa Fluor 647 was used at 1/1,000 dilution. DNA was stained with DAPI and the merged image was shown on right.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab128196 尚未被引用在任何文献中。