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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases

Anti-Raf1 (phospho S621)抗体(ab4767)

  • Datasheet
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Western blot - Anti-Raf1 (phospho S621) antibody (ab4767)

    Key features and details

    • Rabbit polyclonal to Raf1 (phospho S621)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-Raf1 (phospho S621) antibody [EPR1521(2)] (ab157201)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-Raf1 (phospho S621)抗体
      参阅全部 Raf1 一抗
    • 描述

      兔多克隆抗体to Raf1 (phospho S621)
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat
    • 免疫原

      Synthetic peptide corresponding to Human Raf1 (phospho S621).
      Database link: P04049

    • 阳性对照

      • Immunoprecipitates or cell lysates from EGF-stimulated Hek293 cells transfected with c-Raf.
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 0.1% BSA

      BSA is IgG and protease free
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated c-Raf. The final product is generated by affinity chromatography using a c-Raf-derived peptide that is phosphorylated at serine 621.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • Other Kinases
      • Cell Biology
      • Apoptosis
      • Mitochondrial
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Growth factor receptors
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial markers
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Apoptosis
      • Cancer
      • Cell Death
      • Apoptosis
      • Mitochondrial
      • Cancer
      • Cell Death
      • Apoptosis
      • Metabolism

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Raf1 protein (ab90536)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab4767于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/1000. Predicted molecular weight: 74 kDa.
    说明
    WB
    1/1000. Predicted molecular weight: 74 kDa.

    靶标

    • 功能

      Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
    • 组织特异性

      In skeletal muscle, isoform 1 is more abundant than isoform 2.
    • 疾病相关

      Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
      Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
    • 序列相似性

      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
      Contains 1 RBD (Ras-binding) domain.
    • 翻译后修饰

      Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity.
    • 细胞定位

      Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes.
    • Target information above from: UniProt accession P04049 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 5894 Human
      • Entrez Gene: 110157 Mouse
      • Entrez Gene: 24703 Rat
      • Omim: 164760 Human
      • SwissProt: P04049 Human
      • SwissProt: Q99N57 Mouse
      • SwissProt: P11345 Rat
      • Unigene: 159130 Human
      • Unigene: 184163 Mouse
      • Unigene: 33262 Rat
      see all
    • 别名

      • c Raf antibody
      • C-raf antibody
      • C-Raf proto-oncogene, serine/threonine kinase antibody
      • CMD1NN antibody
      • Craf 1 transforming gene antibody
      • cRaf antibody
      • Craf1 transforming gene antibody
      • EC 2.7.11.1 antibody
      • kinase Raf1 antibody
      • Murine sarcoma 3611 oncogene 1 antibody
      • NS5 antibody
      • Oncogene MIL antibody
      • Oncogene RAF1 antibody
      • OTTHUMP00000160218 antibody
      • OTTHUMP00000207813 antibody
      • OTTHUMP00000209389 antibody
      • Protein kinase raf 1 antibody
      • Proto-oncogene c-RAF antibody
      • Raf 1 antibody
      • Raf 1 proto oncogene serine/threonine kinase antibody
      • RAF antibody
      • Raf proto oncogene serine/threonine protein kinase antibody
      • RAF proto-oncogene serine/threonine-protein kinase antibody
      • RAF-1 antibody
      • RAF1 antibody
      • RAF1_HUMAN antibody
      • Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody
      • TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody
      • v raf 1 murine leukemia viral oncogene homolog 1 antibody
      • v-raf murine sarcoma viral oncogene homolog 1 antibody
      • v-raf-1 murine leukemia viral oncogene-like protein 1 antibody
      • vraf1 murine leukemia viral oncogene homolog 1 antibody
      see all

    图片

    • Western blot - Anti-Raf1 (phospho S621) antibody (ab4767)
      Western blot - Anti-Raf1 (phospho S621) antibody (ab4767)
      Peptide Competition and Mutant Analysis:
      Immunoprecipitates prepared from Hek293 cells stimulated with EGF and overexpressing wild-type c-Raf (1-4) or c-Raf mutant S621A (5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4767 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: the phosphopeptide immunogen (1), a generic phosphoserine containing peptide (2), the non-phosphopeptide corresponding to the immunogen (3), or, no peptide (4, 5). The smaller blot presented below shows the relative amount of protein in the wild-type vs. the S621A mutant protein via a c-Raf pan antibody. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide c

    实验方案

    • Peptide Competition Experiment
    • Western blotting protocol

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (1)

    发表研究结果有使用 ab4767?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4767 被引用在 1 文献中.

    • Hasegawa T  et al. ErbB2 signaling epigenetically suppresses microRNA-205 transcription via Ras/Raf/MEK/ERK pathway in breast cancer. FEBS Open Bio 7:1154-1165 (2017). PubMed: 28781955

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