AB4767

Anti-Raf1 (phospho S621)抗体

Anti-Raf1 (phospho S621) antibody

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(3 Publications)

Rabbit Polyclonal RAF1 phospho S621 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RAF1 phospho S621.

查看别名

RAF, RAF1, RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, Raf-1, cRaf

1 Images

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Western blot - Anti-Raf1 (phospho S621) antibody (AB4767)

Peptide Competition and Mutant Analysis :
Immunoprecipitates prepared from Hek293 cells stimulated with EGF and overexpressing wild-type c-Raf (1-4) or c-Raf mutant S621A (5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4^oC, then were incubated with 0.50 μg/mL ab4767 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with : the phosphopeptide immunogen (1), a generic phosphoserine containing peptide (2), the non-phosphopeptide corresponding to the immunogen (3), or, no peptide (4, 5). The smaller blot presented below shows the relative amount of protein in the wild-type vs. the S621A mutant protein via a c-Raf pan antibody. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide c

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Western blot - Anti-Raf1 (phospho S621) antibody (ab4767)

Predicted band size: 73 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Synthetic Peptide within Human RAF1 phospho S621. The exact immunogen used to generate this antibody is proprietary information.

P04049

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反应性数据

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated c-Raf. The final product is generated by affinity chromatography using a c-Raf-derived peptide that is phosphorylated at serine 621.

存储溶液

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Raf1 also known as c-Raf or Raf-1 is a serine/threonine-protein kinase that plays an important role in cell division differentiation and survival. Its molecular weight is approximately 74 kDa. Raf1 is part of the MAPK/ERK signaling pathway and is ubiquitously expressed in many tissues including the brain heart and liver. As a component of the signaling cascade its activation translates extracellular signals into intracellular responses.

Biological function summary

Raf1 regulates important cellular processes by activating downstream kinases in response to external stimuli. Raf1 forms a complex with other proteins such as Ras facilitating its role as an essential component of the MAPK/ERK pathway. Upon activation by Ras Raf1 phosphorylates and activates MEK1 and MEK2 which in turn activate the ERK1 and ERK2. This signaling axis is involved in controlling gene expression and cellular proliferation.

Pathways

Raf1 is integrally involved in the MAPK/ERK pathway which is critical for transducing signals from growth factors and mitogens. It relates closely with proteins such as Ras MEK and ERK in this pathway. The pathway is important for regulating cellular responses to various stimuli and is particularly involved in processes such as cell cycle control and apoptosis.

Raf1 has associations with certain types of cancer and Noonan syndrome. In various cancers mutations or dysregulation of Raf1 or related components such as Ras can lead to uncontrolled cellular proliferation. In Noonan syndrome Raf1 mutations result in anomalies in the Ras/MAPK pathway which can impact normal development. Raf1’s interactions in these pathways indicate its relevance as a potential target for therapeutic intervention.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

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靶点信息

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases : ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

See full target information RAF1 phospho S621

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 29:966-977 PubMed36175670

2022

Structure of the SHOC2-MRAS-PP1C complex provides insights into RAF activation and Noonan syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel A Bonsor,Patrick Alexander,Kelly Snead,Nicole Hartig,Matthew Drew,Simon Messing,Lorenzo I Finci,Dwight V Nissley,Frank McCormick,Dominic Esposito,Pablo Rodriguez-Viciana,Andrew G Stephen,Dhirendra K Simanshu

Protein expression and purification 193:106061 PubMed35131438

2022

Polycistronic baculovirus expression of SUGT1 enables high-yield production of recombinant leucine-rich repeat proteins and protein complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly Snead,Vanessa Wall,Hannah Ambrose,Dominic Esposito,Matthew Drew

FEBS open bio 7:1154-1165 PubMed28781955

2017

ErbB2 signaling epigenetically suppresses microRNA-205 transcription via Ras/Raf/MEK/ERK pathway in breast cancer.

Applications