重组Anti-Raf1 (phospho S259)抗体[EPR3433(2)] (ab173539)
![Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)](https://www.abcam.cn/ps/products/173/ab173539/Images/ab173539-506612-antiraf1-phospho-s259-antibody-epr3433-2-western-blot-hek293-human-2.jpg "Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3433(2)] to Raf1 (phospho S259)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Raf1 (phospho S259)抗体[EPR3433(2)]
参阅全部 Raf1 一抗 -
描述
兔单克隆抗体[EPR3433(2)] to Raf1 (phospho S259) -
宿主
Rabbit -
经测试应用
适用于: WB, Dot blotmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Treated C6, NIH/3T3 and HEK-293 whole cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3433(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab173539于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 73 kDa.
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| Dot blot |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 73 kDa. |
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Dot blot
Use at an assay dependent concentration. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.
靶标
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功能
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3. -
组织特异性
In skeletal muscle, isoform 1 is more abundant than isoform 2. -
疾病相关
Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity. -
细胞定位
Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. - Information by UniProt
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数据库链接
- Entrez Gene: 5894 Human
- Entrez Gene: 110157 Mouse
- Entrez Gene: 24703 Rat
- Omim: 164760 Human
- SwissProt: P04049 Human
- SwissProt: Q99N57 Mouse
- SwissProt: P11345 Rat
- Unigene: 159130 Human
see all -
别名
- c Raf antibody
- C-raf antibody
- C-Raf proto-oncogene, serine/threonine kinase antibody
see all
图片
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Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)All lanes : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/5000 dilution (Purified)
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) treated with 100nM Phorbol-12-myristate-13-acetate for 1 hour whole cell lysates
Lane 3 : HEK-293 (Human embryonic kidney epithelial cell) treated with 100nM Phorbol-12-myristate-13-acetate for 1 hour whole cell lysates, then incubated with phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDaab181602 was used as GAPDH loading control.
ab181115 was used to detect Raf1.
ab150365 was used to detect Raf1 phospho S43.
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![Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)](https://www.abcam.cn/ps/products/173/ab173539/Images/ab173539-339709-ab173539WB2.jpg)
Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)All lanes : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/1000 dilution (Purified)
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) treated with 5uM forskolin for 10 minutes whole cell lysates
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) treated with 5uM forskolin for 10 minutes whole cell lysates, then incubated with phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa -
![Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)](https://www.abcam.cn/ps/products/173/ab173539/Images/ab173539-339708-ab173539WB1.jpg)
Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)Lane 1 : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/1000 dilution (Purified)
Lanes 2-3 : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 2 : C6 (Rat glial tumor glial cell) treated with 30uM forskolin for 20 minutes whole cell lysates
Lane 3 : C6 (Rat glial tumor glial cell) treated with 30uM forskolin for 20 minutes whole cell lysates, then incubated with phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa -
![Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)](https://www.abcam.cn/ps/products/173/ab173539/Images/ab173539-276099-anti-raf1-phospho-s259-antibody-epr34332-western-blot.jpg)
Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)All lanes : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/5000 dilution
Lane 1 : HEK293 whole cell lysate
Lane 2 : HEK293 whole cell lysate treated with 100nM Phorbol-12-myristate-13-acetate for 1 hour
Lane 3 : HEK293 whole cell lysate treated with 100nM Phorbol-12-myristate-13-acetate for 1 hour then incubated with phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa
Exposure time: 1 secondBlocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)](https://www.abcam.cn/ps/products/173/ab173539/Images/ab173539-276098-anti-raf1-phospho-s259-antibody-epr34332-dot-blot.jpg)
Dot Blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)Dot blot analysis of Raf1 (pS259) peptide (Lane 1) and Raf1 non-phospho peptide (Lane 2) labelling Raf1 (pS259) with ab173539 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)](https://www.abcam.cn/ps/products/173/ab173539/Images/ab173539-197083-anti-raf1-phospho-s259-antibody-epr34332-western-blot.jpg)
Western blot - Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)All lanes : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/1000 dilution
Lane 1 : 293T cell lysates
Lane 2 : 293T cell lysates treated with Okadaic Acid + Calyculin A
Lysates/proteins at 10 µg per lane.
Predicted band size: 73 kDa -
![Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)](https://www.abcam.cn/ps/products/173/ab173539/Images/ab173539-9-benefits-of-recombinant-antibodies.png)
Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539)
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab173539 被引用在 17 文献中.
- Liu YF et al. Xiaozheng pill exerts an anti-mammary hyperplasia effect through Raf/ERK/ELK and HIF-1α/bFGF pathways. J Tradit Complement Med 13:600-610 (2023). PubMed: 38020551
- Kong D et al. Procoxacin bidirectionally inhibits osteoblastic and osteoclastic activity in bone and suppresses bone metastasis of prostate cancer. J Exp Clin Cancer Res 42:45 (2023). PubMed: 36759880
- Snead K et al. Polycistronic baculovirus expression of SUGT1 enables high-yield production of recombinant leucine-rich repeat proteins and protein complexes. Protein Expr Purif 193:106061 (2022). PubMed: 35131438
- Bonsor DA et al. Structure of the SHOC2-MRAS-PP1C complex provides insights into RAF activation and Noonan syndrome. Nat Struct Mol Biol 29:966-977 (2022). PubMed: 36175670
- Li W et al. 4,5-Dimethoxycanthin-6-one is a novel LSD1 inhibitor that inhibits proliferation of glioblastoma cells and induces apoptosis and pyroptosis. Cancer Cell Int 22:32 (2022). PubMed: 35042538