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重组RabMAb

重组Anti-Radixin/RDX抗体[EP1862Y] (ab52495)

Reviews (4) Submit a question References (22)
Western blot - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
  • Immunocytochemistry/ Immunofluorescence - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
  • Flow Cytometry (Intracellular) - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
  • Anti-Radixin/RDX antibody [EP1862Y] (ab52495)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1862Y] to Radixin/RDX
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Radixin/RDX抗体[EP1862Y]
    参阅全部 Radixin/RDX 一抗

  • 描述

    兔单克隆抗体[EP1862Y] to Radixin/RDX

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human Radixin/RDX aa 350-450 (N terminal). The exact sequence is proprietary.

  • 阳性对照

    • WB: HeLa cell lysate. IHC-P: Paraffin embedded human ovarian carcinoma. Flow Cyt (intra): HeLa cells. ICC/IF: Mcf7 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • 存储溶液

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EP1862Y

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab52495于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF (2)
Use a concentration of 5 µg/ml.
WB (2)
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
Use a concentration of 5 µg/ml.
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能

    Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.

  • 疾病相关

    Defects in RDX are the cause of deafness autosomal recessive type 24 (DFNB24) [MIM:611022]. DFNB24 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

  • 序列相似性

    Contains 1 FERM domain.

  • 结构域

    The N-terminal domain interacts with the C-terminal domain of LAYN. An interdomain interaction between its N-terminal and C-terminal domains inhibits its ablilty to bind LAYN. In the presence of acidic phospholipids, the interdomain interaction is inhibited and this enhances binding to LAYN.

  • 翻译后修饰

    Phosphorylated by tyrosine-protein kinases.

  • 细胞定位

    Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • CB567 antibody
    • CG12537 antibody
    • DFNB24 antibody
    • ESP10 antibody
    • Hh-induced MATH and BTB domain-containing protein antibody
    • HIB antibody
    • Moesin-B antibody
    • Protein roadkill antibody
    • RADI_HUMAN antibody
    • Radixin antibody
    • RDX antibody
    see all

图片

  • Western blot - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
    Western blot - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
    Anti-Radixin/RDX antibody [EP1862Y] (ab52495) at 1/1000 dilution + HeLa cell lysate at 10 µg

    Secondary
    HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 80 kDa
    Observed band size: 80 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)

    Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using ab52495 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
    Immunocytochemistry/ Immunofluorescence - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
    ICC/IF image of ab52495 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52495, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow Cytometry (Intracellular) - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
    Flow Cytometry (Intracellular) - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)

    Overlay histogram showing HeLa cells stained with ab52495 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52495, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. 

     

     

  • Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
    Anti-Radixin/RDX antibody [EP1862Y] (ab52495)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (22)

发表研究结果有使用 ab52495？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab52495 被引用在 22 文献中.

  • Seker U  et al. Effects of black cumin seed oil on oxidative stress and expression of membrane-cytoskeleton linker proteins, radixin, and moesin in streptozotocin-induced diabetic rat liver. Hepatol Forum 3:21-26 (2022). PubMed: 35782372
  • Zhang Y  et al. Oocyte-derived microvilli control female fertility by optimizing ovarian follicle selection in mice. Nat Commun 12:2523 (2021). PubMed: 33953177
  • Zhang L  et al. STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells. Exp Ther Med 22:851 (2021). PubMed: 34149897
  • Zhang L  et al. Knockout of STK10 promotes the migration and invasion of cervical cancer cells. Transl Cancer Res 9:7079-7090 (2020). PubMed: 35117313
  • Zhang N  et al. LncRNA linc01116 prometes glioma cell migration and invasion by modulation of radixin targeted by miR-31. Int J Clin Exp Pathol 12:1078-1086 (2019). PubMed: 31933922
View all Publications for this product

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Radixin/RDX antibody [EP1862Y]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - Tx100
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Jerome Cattin

Verified customer

提交于 Jun 25 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Radixin/RDX antibody [EP1862Y]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS (Human Bone Osteosarcoma Epithelial Cells))
Permeabilization
Yes - 0.5% triton-X100
Specification
U2OS (Human Bone Osteosarcoma Epithelial Cells)
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 16 2020

Western blot abreview for Anti-Radixin/RDX antibody [EP1862Y]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
293T
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Jerome Cattin

Verified customer

提交于 May 20 2020

Western blot abreview for Anti-Radixin antibody [EP1862Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount
30 µg
Specification
NIH 3T3 cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 27 2013

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