Anti-RAD54抗体(ab10705)
Key features and details
- Rabbit polyclonal to RAD54
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RAD54抗体
参阅全部 RAD54 一抗 -
描述
兔多克隆抗体to RAD54 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IPmore details
不适用于: ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- This antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293 as well as HeLa Nuclear lysate in Western blot. This antibody also gave a positive signal in Immunohistochemistry within Human breast adenocarcinoma formalin fixed paraffin embedded tissue section.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10705于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 84.3 kDa).
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| IP |
Use a concentration of 5 µg/ml.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 84.3 kDa). |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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IP
Use a concentration of 5 µg/ml. |
靶标
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功能
Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). May play also an essential role in telomere length maintenance and telomere capping in mammalian cells. -
序列相似性
Belongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8438 Human
- Omim: 603615 Human
- SwissProt: Q92698 Human
- Unigene: 642042 Human
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别名
- DNA repair and recombination protein RAD54 like antibody
- DNA repair and recombination protein RAD54-like antibody
- hHR 54 antibody
see all
图片
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Western blot - Anti-RAD54 antibody (ab10705)Lanes 2-6 : Anti-RAD54 antibody (ab10705) at 1/500 dilution
Lane 1 : Marker
Lane 2 : HeLa nuclear cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A432 cell lysate
Lane 5 : MCF7 cell lysate
Lane 6 : Hek 293 cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
Predicted band size: 84.3 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Rabbit polyclonal to RAD54 (ab10705) at 1/500.
Lane 1 Marker
Lane 2 HeLa nuclear extract
Lane 3 HeLa cell lysate
Lane 4 A432 cell lysate
Lane 5 MCF7 cell lysate
Lane 6 HEK 293 cell lysateSecondary antibody - Goat polyclonal to rabbit IgG (HRP) - ab6721.
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Immunoprecipitation - Anti-RAD54 antibody (ab10705)RAD54 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to RAD54 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab10705.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 70kDa; RAD54, non specific band: 75 kDa, We are unsure as to the identity of this extra band. 99kDa band:due to background seen in No ab control lane (2). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAD54 antibody (ab10705)IHC image of ab10705 staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10705, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab10705 被引用在 3 文献中.
- Mun JY et al. E2F1 Promotes Progression of Bladder Cancer by Modulating RAD54L Involved in Homologous Recombination Repair. Int J Mol Sci 21:N/A (2020). PubMed: 33261027
- Tripathi V et al. MRN complex-dependent recruitment of ubiquitylated BLM helicase to DSBs negatively regulates DNA repair pathways. Nat Commun 9:1016 (2018). PubMed: 29523790
- Oji Y et al. Wilms' tumor gene WT1 promotes homologous recombination-mediated DNA damage repair. Mol Carcinog 54:1758-71 (2015). PubMed: 25418835