重组Anti-RAD52抗体[EPR3464(2)] (ab124971)
![Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)](https://www.abcam.cn/ps/products/124/ab124971/Images/ab124971-351366-anti-rad52-antibody-epr3464-2-westernblot-human.jpg "Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3464(2)] to RAD52
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-RAD52抗体[EPR3464(2)]
参阅全部 RAD52 一抗 -
描述
兔单克隆抗体[EPR3464(2)] to RAD52 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human RAD52 (C terminal). The exact sequence is proprietary.
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阳性对照
- WB: HeLa, HEK-293T and PC3 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解离常数（KD）
KD = 3.00 x 10 -12 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3464(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124971于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa).
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa). |
应用说明
Is unsuitable for ICC or IHC-P.
靶标
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功能
Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase. -
序列相似性
Belongs to the RAD52 family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5893 Human
- Omim: 600392 Human
- SwissProt: P43351 Human
- Unigene: 410355 Human
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别名
- DNA repair protein RAD52 antibody
- DNA repair protein RAD52 antibody
- DNA repair protein RAD52 homolog antibody
see all
图片
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Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)Lane 1 : Anti-RAD52 antibody [EPR3464(2)] (ab124971) at 1/1000 dilution
Lane 2 : Anti-RAD52 antibody [EPR3464(2)] (ab124971) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)](https://www.abcam.cn/ps/products/124/ab124971/Images/ab124971-139059-anti-rad52-antibody-epr34642-western-blot.jpg)
Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)All lanes : Anti-RAD52 antibody [EPR3464(2)] (ab124971) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 46 kDa -
![OI-RD Scanning - Anti-RAD52 antibody [EPR3464(2)] (ab124971)](https://www.abcam.cn/ps/products/124/ab124971/Images/ab124971-139058-anti-rad52-antibody-epr34642.jpg)
OI-RD Scanning - Anti-RAD52 antibody [EPR3464(2)] (ab124971) -
![Anti-RAD52 antibody [EPR3464(2)] (ab124971)](https://www.abcam.cn/ps/products/124/ab124971/Images/ab124971-4-benefits-of-recombinant-antibodies.png)
Anti-RAD52 antibody [EPR3464(2)] (ab124971)
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab124971 被引用在 9 文献中.
- Yadav T et al. TERRA and RAD51AP1 promote alternative lengthening of telomeres through an R- to D-loop switch. Mol Cell 82:3985-4000.e4 (2022). PubMed: 36265486
- Zhang JM et al. Alternative lengthening of telomeres is a self-perpetuating process in ALT-associated PML bodies. Mol Cell 81:1027-1042.e4 (2021). PubMed: 33453166
- Lippert TP et al. Oncogenic herpesvirus KSHV triggers hallmarks of alternative lengthening of telomeres. Nat Commun 12:512 (2021). PubMed: 33479235
- Liu X et al. Chromosomal aberration arises during somatic reprogramming to pluripotent stem cells. Cell Div 15:12 (2020). PubMed: 33292330
- Hanamshet K & Mazin AV The function of RAD52 N-terminal domain is essential for viability of BRCA-deficient cells. Nucleic Acids Res 48:12778-12791 (2020). PubMed: 33275133
- Gong H et al. p73 coordinates with ?133p53 to promote DNA double-strand break repair. Cell Death Differ N/A:N/A (2018). PubMed: 29511339
- Tong L et al. RDM1 plays an oncogenic role in human lung adenocarcinoma cells. Sci Rep 8:11525 (2018). PubMed: 30069034
- Yasuhara T et al. Human Rad52 Promotes XPG-Mediated R-loop Processing to Initiate Transcription-Associated Homologous Recombination Repair. Cell 175:558-570.e11 (2018). PubMed: 30245011
- Gong L et al. p53 isoform ?133p53 promotes efficiency of induced pluripotent stem cells and ensures genomic integrity during reprogramming. Sci Rep 6:37281 (2016). PubMed: 27874035