AB202063

重组Anti-Rad51D抗体[EPR16205]

Anti-Rad51D antibody [EPR16205]

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(8 Publications)

Rabbit Recombinant Monoclonal Rad51D antibody. Suitable for IP, WB, IHC-P and reacts with Human, Rat samples. Cited in 8 publications.

查看别名

RAD51L3, RAD51D, DNA repair protein RAD51 homolog 4, R51H3, RAD51 homolog D, RAD51-like protein 3, TRAD

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (AB202063)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (AB202063)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling Rad51D with ab202063 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human lung carcinoma tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (AB202063)

Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling Rad51D with ab202063 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Nuclear staining on Human small intestine tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (AB202063)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Rad51D with ab202063 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Nuclear staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Rad51D antibody [EPR16205] (AB202063)

Rad51D was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab202063 at 1/50 dilution.

Western blot was performed from the immunoprecipitate using ab202063 at 1/1000 dilution.

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.

Lane 1 : HeLa whole cell lysate 10 μg (Input).

Lane 2 : ab202063 IP in HeLa whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab202063 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Rad51D antibody [EPR16205] (ab202063)

Predicted band size: 35 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] (AB202063)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Rad51D with ab202063 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Nuclear staining on rat kidney tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Rad51D antibody [EPR16205] (AB202063)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Rad51D antibody [EPR16205] (ab202063) at 1/1000 dilution

Lane 1:

HEK-293 (Human epithelial cells from embryonic kidney) cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

Lane 3:

Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 35 kDa

false

Exposure time: 30s

Unknown

Western blot - Anti-Rad51D antibody [EPR16205] (AB202063)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Rad51D antibody [EPR16205] (ab202063) at 1/1000 dilution

Lane 1:

Rat kidney lysate at 10 µg

Lane 2:

C6 (Rat glial tumor cells) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 35 kDa

false

Exposure time: 3min

不同偶联物与剂型 (1)

Carrier free

Anti-Rad51D antibody [EPR16205] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR16205

亚型

IgG

不含载体蛋白

反应种属

Rat, Human

应用

IHC-P, IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Rad51D also known as RAD51L3 is a protein involved in homologous recombination repair of DNA. It has a molecular weight of approximately 37 kDa. Rad51D is primarily expressed in tissues with high cell division rates such as the testis and thymus. This protein belongs to the RAD51 family which is responsible for repairing DNA double-strand breaks (DSBs) that can arise during replication or after exposure to genotoxic agents. Rad51D's role centers on promoting the exchange of DNA strands during repair processes which helps maintain genome stability.

Biological function summary

Rad51D plays a significant role in maintaining genomic integrity by facilitating homologous recombination. It interacts with Rad51 paralogs like Rad51C as part of the BCDX2 complex which also includes Rad51B and XRCC2. This complex is essential in the early steps of homologous recombination especially in forming the synaptic complex that searches for homology. The interactions among these proteins enable efficient strand invasion and exchange essential for error-free DNA repair.

Pathways

Rad51D is an important component in DNA repair and damage response pathways. It primarily functions within the homologous recombination repair pathway. In this pathway Rad51D works closely with the RAD52 epistasis group including proteins such as BRCA1 and BRCA2 which assist in the proper localization and functioning of RAD51 recombinase. Rad51D's involvement in these pathways ensures accurate and effective DNA repair preventing genomic instability that could lead to cellular dysfunction.

Rad51D mutations have been linked to a higher risk of breast and ovarian cancer. Its association with the BRCA1 and BRCA2 proteins suggests a shared pathway in hereditary breast and ovarian cancer syndromes where deficient DNA repair leads to genomic mutations and tumorigenesis. Further research continues to explore Rad51D's potential as a biomarker for cancer susceptibility and its utility in developing targeted therapies.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2 : RAD51D can stimulate Holliday junction resolution by BLM.

See full target information RAD51D

style

left-column

文献 (8)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37833926

2023

RAD51D Secondary Mutation-Mediated Resistance to PARP-Inhibitor-Based Therapy in HGSOC.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xu,Yilin Dai,Yi Gao,Ranran Chai,Chong Lu,Bing Yu,Yu Kang,Congjian Xu

Human molecular genetics 31:4241-4254 PubMed35904444

2022

Identification of new RAD51D-regulating microRNAs that also emerge as potent inhibitors of the Fanconi anemia/homologous recombination pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Hater,Katharina M Iwaniuk,Carina Leifeld,Pia Grüten,Constanze Wiek,Katharina Raba,Fan Zhang,Johannes C Fischer,Paul R Andreassen,Helmut Hanenberg,Hans-Ingo Trompeter

Nucleic acids research 50:8008-8022 PubMed35801922

2022

Strand annealing and motor driven activities of SMARCAL1 and ZRANB3 are stimulated by RAD51 and the paralog complex.

Applications

Unspecified application

Species

Unspecified reactive species

Swagata Halder,Lepakshi Ranjha,Angelo Taglialatela,Alberto Ciccia,Petr Cejka

Cancers 14: PubMed35565380

2022

The Genetic and Molecular Analyses of and Identifies Rare Variants Implicated in Hereditary Ovarian Cancer from a Genetically Unique Population.

Applications

Unspecified application

Species

Unspecified reactive species

Wejdan M Alenezi,Larissa Milano,Caitlin T Fierheller,Corinne Serruya,Timothée Revil,Kathleen K Oros,Supriya Behl,Suzanna L Arcand,Porangana Nayar,Dan Spiegelman,Simon Gravel,Anne-Marie Mes-Masson,Diane Provencher,William D Foulkes,Zaki El Haffaf,Guy Rouleau,Luigi Bouchard,Celia M T Greenwood,Jean-Yves Masson,Jiannis Ragoussis,Patricia N Tonin

Nature communications 11:3531 PubMed32669601

2020

Sequential role of RAD51 paralog complexes in replication fork remodeling and restart.

Applications

Unspecified application

Species

Unspecified reactive species

Matteo Berti,Federico Teloni,Sofija Mijic,Sebastian Ursich,Jevgenij Fuchs,Maria Dilia Palumbieri,Jana Krietsch,Jonas A Schmid,Edwige B Garcin,Stéphanie Gon,Mauro Modesti,Matthias Altmeyer,Massimo Lopes

Aging 10:1556-1574 PubMed30001218

2018

Nicotine exposure impairs germ cell development in human fetal ovaries cultured .

Applications

Unspecified application

Species

Unspecified reactive species

Shun-Feng Cheng,Xun-Si Qin,Ze-Li Han,Xiao-Feng Sun,Yan-Ni Feng,Fan Yang,Wei Ge,Lan Li,Yong Zhao,Massimo De Felici,Shu-Hua Zou,Yi Zhou,Wei Shen

Cancer research 77:4517-4529 PubMed28646019

2017

Functionally Null Missense Mutation Associates Strongly with Ovarian Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Rivera,Massimo Di Iorio,Jessica Frankum,Javad Nadaf,Somayyeh Fahiminiya,Suzanna L Arcand,David L Burk,Damien Grapton,Eva Tomiak,Valerie Hastings,Nancy Hamel,Rabea Wagener,Olga Aleynikova,Sylvie Giroux,Fadi F Hamdan,Alexandre Dionne-Laporte,George Zogopoulos,Francois Rousseau,Albert M Berghuis,Diane Provencher,Guy A Rouleau,Jacques L Michaud,Anne-Marie Mes-Masson,Jacek Majewski,Susanne Bens,Reiner Siebert,Steven A Narod,Mohammad R Akbari,Christopher J Lord,Patricia N Tonin,Alexandre Orthwein,William D Foulkes

Cancer discovery 7:984-998 PubMed28588062

2017

Secondary Somatic Mutations Restoring and Associated with Acquired Resistance to the PARP Inhibitor Rucaparib in High-Grade Ovarian Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Olga Kondrashova,Minh Nguyen,Kristy Shield-Artin,Anna V Tinker,Nelson N H Teng,Maria I Harrell,Michael J Kuiper,Gwo-Yaw Ho,Holly Barker,Maria Jasin,Rohit Prakash,Elizabeth M Kass,Meghan R Sullivan,Gregory J Brunette,Kara A Bernstein,Robert L Coleman,Anne Floquet,Michael Friedlander,Ganessan Kichenadasse,David M O'Malley,Amit Oza,James Sun,Liliane Robillard,Lara Maloney,David Bowtell,Heidi Giordano,Matthew J Wakefield,Scott H Kaufmann,Andrew D Simmons,Thomas C Harding,Mitch Raponi,Iain A McNeish,Elizabeth M Swisher,Kevin K Lin,Clare L Scott

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Rad51D抗体[EPR16205]