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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Homologous Recomb.

Anti-Rad51C抗体(ab3669)

  • Datasheet
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Western blot - Anti-Rad51C antibody (ab3669)

    Key features and details

    • Goat polyclonal to Rad51C
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-Rad51C抗体
      参阅全部 Rad51C 一抗
    • 描述

      山羊多克隆抗体to Rad51C
    • 宿主

      Goat
    • 特异性

      This antibody is expected to recognise all three human isoforms.
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Synthetic peptide:

      RGKTFRFEMQRDL

      , corresponding to N terminal amino acids 2-14 of Human Rad51C.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 阳性对照

      • HeLa lysate.
    • 常规说明

      Principal Names – RAD51C; RAD51L2; RAD51 homolog C (S. cerevisiae); yeast RAD51 homolog 3; DNA repair protein RAD51 homolog 3 Official Gene Symbol - RAD51C GenBank Accession Number – NP_002867; NP_478123; NP_478124 LocusLink ID - 5889 (human) Gene Ontology terms - adenosinetriphosphatase activity; DNA binding activity; DNA repair; DNA recombination; nucleus.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% Tris, 0.5% BSA
    • Concentration information loading...
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • Homologous Recomb.

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3669于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).

    1 hour primary incubation is recommended for this product.

    说明
    WB
    Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).

    1 hour primary incubation is recommended for this product.

    靶标

    • 功能

      Essential for the homologous recombination (HR) pathway of DNA repair. Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The RAD51B-RAD51C dimer exhibits single-stranded DNA-dependent ATPase activity. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Participates in branch migration and Holliday junction resolution and thus is important for processing HR intermediates late in the DNA repair process. Also has an early function in DNA repair in facilitating phosphorylation of the checkpoint kinase CHK2 and thereby transduction of the damage signal, leading to cell cycle arrest and HR activation. Protects RAD51 from ubiquitin-mediated degradation that is enhanced following DNA damage. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and XRCC3. Contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability. Involved in maintaining centrosome number in mitosis.
    • 组织特异性

      Expressed in a variety of tissues, with highest expression in testis, heart muscle, spleen and prostate.
    • 疾病相关

      Defects in RAD51C are the cause of Fanconi anemia complementation group O (FANCO) [MIM:613390]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
      Defects in RAD51C are the cause of breast-ovarian cancer familial type 3 (BROVCA3) [MIM:613399]. It is a condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate.
    • 序列相似性

      Belongs to the RecA family. RAD51 subfamily.
    • 细胞定位

      Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. DNA damage induces an increase in nuclear levels. Accumulates in DNA damage induced nuclear foci or RAD51C foci which is formed during the S or G2 phase of cell cycle. Accumulation at DNA lesions requires the presence of NBN/NBS1, ATM and RPA.
    • Target information above from: UniProt accession O43502 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 5889 Human
      • Omim: 602774 Human
      • SwissProt: O43502 Human
      • Unigene: 412587 Human
      • 别名

        • BROVCA3 antibody
        • DNA repair protein RAD51 homolog 3 antibody
        • FANCO antibody
        • MGC104277 antibody
        • R51H3 antibody
        • RA51C_HUMAN antibody
        • Rad 51C antibody
        • RAD51 homolog C (S. cerevisiae) antibody
        • Rad51 homolog c (S. cerevisiae), isoform CRA_b antibody
        • RAD51 homolog C antibody
        • RAD51 like protein 2 antibody
        • RAD51 paralog C antibody
        • RAD51, S. cerevisiae, homolog of, C antibody
        • RAD51-like protein 2 antibody
        • RAD51C antibody
        • RAD51C protein antibody
        • RAD51L2 antibody
        • RAD51L2/RAD51C protein antibody
        • RGD1563765 antibody
        • Yeast RAD51 homolog 3 antibody
        see all

      图片

      • Western blot - Anti-Rad51C antibody (ab3669)
        Western blot - Anti-Rad51C antibody (ab3669)
        ab3669 staining (2µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 12 hours.  Detected by western blot using chemiluminescence. ab3669 staining (2µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.

      实验方案

      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (2)

      发表研究结果有使用 ab3669?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab3669 被引用在 2 文献中.

      • Makkonen H  et al. Long-range activation of FKBP51 transcription by the androgen receptor via distal intronic enhancers. Nucleic Acids Res 37:4135-48 (2009). PubMed: 19433513
      • Katsura M  et al. The ATR-Chk1 pathway plays a role in the generation of centrosome aberrations induced by Rad51C dysfunction. Nucleic Acids Res 37:3959-68 (2009). PubMed: 19403737

      客户评价及客户问答

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      提交评价 提交问题

      Question

      I will be starting to do Western Blots for some of these proteins and since no one in my lab works with them, I will need to try out different antibodies to determine which one works best for my cell lines. I would like to know if you could offer me some free samples or discounts on the first order of the following antibodies: Ab3669-goat anti-human Rad51C polyclonal antibody Ab2180-mouse anti-human Rad51C monoclonal antibody Once I determine which antibody works best I will probably use it on a regular basis for the next 2-3 years.

      Read More

      Abcam community

      Verified customer

      Asked on Jan 09 2006

      Answer

      Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Both ab2180 and ab3669 are guaranteed to work in Western blotting with human samples. Should you decide to go ahead and purchase one of these products, please let us know how you get on by submitting an Abreview and in return we will offer you 50 Abpoints which can be redeemed on a number of rewards (a further 100 Abpoints will be offered for an image). Please contact us again if you have any additional questions.

      Read More

      Abcam Scientific Support

      回复于 Jan 10 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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