重组Anti-Rad50抗体[EPR3466(2)] (ab124682)
![Western blot - Anti-Rad50 antibody [EPR3466(2)] (ab124682)](https://www.abcam.cn/ps/products/124/ab124682/Images/ab124682-296770-anti-rad50-antibody-epr34662-western-blot.jpg "Western blot - Anti-Rad50 antibody [EPR3466(2)] (ab124682)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3466(2)] to Rad50
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Rad50抗体[EPR3466(2)]
参阅全部 Rad50 一抗 -
描述
兔单克隆抗体[EPR3466(2)] to Rad50 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt (Intra) or ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- K562, MOLT4, Jurkat and MCF7 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3466(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124682于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 154 kDa).
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| 说明 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 154 kDa). |
应用说明
Is unsuitable for Flow Cyt (Intra) or ICC/IF.
靶标
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功能
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. -
组织特异性
Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts. -
疾病相关
Defects in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]; also called NBS-like disorder or RAD50 deficiency. NBSLD is a disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent. -
序列相似性
Belongs to the SMC family. RAD50 subfamily.
Contains 1 zinc-hook domain. -
结构域
The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. Chromosome > telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents. - Information by UniProt
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数据库链接
- Entrez Gene: 10111 Human
- Entrez Gene: 19360 Mouse
- Entrez Gene: 64012 Rat
- Omim: 604040 Human
- SwissProt: Q92878 Human
- SwissProt: P70388 Mouse
- SwissProt: Q9JIL8 Rat
- Unigene: 633509 Human
see all -
别名
- DNA repair protein RAD50 antibody
- hRAD50 antibody
- hsRAD50 antibody
see all
图片
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Western blot - Anti-Rad50 antibody [EPR3466(2)] (ab124682)All lanes : Anti-Rad50 antibody [EPR3466(2)] (ab124682) at 1/5000 dilution (purified)
Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 154 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-Rad50 antibody [EPR3466(2)] (ab124682)](https://www.abcam.cn/ps/products/124/ab124682/Images/ab124682-296769-anti-rad50-antibody-epr34662-western-blot.jpg)
Western blot - Anti-Rad50 antibody [EPR3466(2)] (ab124682)All lanes : Anti-Rad50 antibody [EPR3466(2)] (ab124682) at 1/1000 dilution (purified)
Lane 1 : Human fetal brain lysates
Lane 2 : Mouse thymus lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 154 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-Rad50 antibody [EPR3466(2)] (ab124682)](https://www.abcam.cn/ps/products/124/ab124682/Images/ab124682-296771-anti-rad50-antibody-epr34662-western-blot.jpg)
Western blot - Anti-Rad50 antibody [EPR3466(2)] (ab124682)Anti-Rad50 antibody [EPR3466(2)] (ab124682) at 1/1000 dilution (purified) + Rat testis lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 154 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-Rad50 antibody [EPR3466(2)] (ab124682)](https://www.abcam.cn/ps/products/124/ab124682/Images/ab124682-138428-anti-rad50-antibody-epr34662-western-blot.jpg)
Western blot - Anti-Rad50 antibody [EPR3466(2)] (ab124682)All lanes : Anti-Rad50 antibody [EPR3466(2)] (ab124682) at 1/1000 dilution (unpurified)
Lane 1 : K562 cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 154 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
![Anti-Rad50 antibody [EPR3466(2)] (ab124682)](https://www.abcam.cn/ps/products/124/ab124682/Images/ab124682-8-benefits-of-recombinant-antibodies.png)
Anti-Rad50 antibody [EPR3466(2)] (ab124682)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab124682 被引用在 9 文献中.
- Li KQ et al. EPAS1 prevents telomeric damage-induced senescence by enhancing transcription of TRF1, TRF2, and RAD50. Zool Res 44:636-649 (2023). PubMed: 37070589
- Tao L et al. Repression of LSD1 potentiates homologous recombination-proficient ovarian cancer to PARP inhibitors through down-regulation of BRCA1/2 and RAD51. Nat Commun 14:7430 (2023). PubMed: 37973845
- Yu M et al. Targeting type Iγ phosphatidylinositol phosphate kinase overcomes oxaliplatin resistance in colorectal cancer. Theranostics 12:4386-4398 (2022). PubMed: 35673560
- Tang D et al. MYC/NBS1-Mediated DNA Damage Response is Involved in the Inhibitory Effect of Hydroxysafflor Yellow A on Glioma Cells. Drug Des Devel Ther 15:1749-1763 (2021). PubMed: 33953544
- Ye Z et al. GRB2 enforces homology-directed repair initiation by MRE11. Sci Adv 7:N/A (2021). PubMed: 34348893
- Ren S et al. ATM-mediated DNA double-strand break response facilitated oncolytic Newcastle disease virus replication and promoted syncytium formation in tumor cells. PLoS Pathog 16:e1008514 (2020). PubMed: 32479542
- Legrand JMD et al. DDX5 plays essential transcriptional and post-transcriptional roles in the maintenance and function of spermatogonia. Nat Commun 10:2278 (2019). PubMed: 31123254
- Chen Y et al. EGFR tyrosine kinase inhibitor HS-10182 increases radiation sensitivity in non-small cell lung cancers with EGFR T790M mutation. Cancer Biol Med 15:39-51 (2018). PubMed: 29545967
- Sun C et al. BRD4 Inhibition Is Synthetic Lethal with PARP Inhibitors through the Induction of Homologous Recombination Deficiency. Cancer Cell 33:401-416.e8 (2018). PubMed: 29533782