Anti-Rad21抗体(ab992)
Key features and details
- Rabbit polyclonal to Rad21
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Rad21抗体
参阅全部 Rad21 一抗 -
描述
兔多克隆抗体to Rad21 -
宿主
Rabbit -
特异性
The epitope recognized by Anti-Rad21 antibody (ab992) maps to a region between residue 575 and the C-terminus (residue 631) human Rad21 homolog using the numbering given in entry NP_006256.1 (GeneID 5885).
-
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Gorilla, Chinese hamster, Orangutan, Elephant
-
免疫原
Synthetic peptide corresponding to Human Rad21 (C terminal) conjugated to keyhole limpet haemocyanin. Represented a portion of human Rad21 encoded within exon 14 (LocusLink ID 5885).
-
阳性对照
- WB: HeLa, Jurkat, HEK293T, RKO, GaMG, MCF-7, A-549, U2OS, NIH/3T3, and TCMK-1 whole cell lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | (1) |
Use at an assay dependent concentration.
ab992 used for IP at 6 µg per reaction. |
| WB | (7) |
1/500 - 1/5000. Predicted molecular weight: 72 kDa.
Band observed at ~130 kDa. |
| 说明 |
|---|
|
IP
Use at an assay dependent concentration. ab992 used for IP at 6 µg per reaction. |
|
WB
1/500 - 1/5000. Predicted molecular weight: 72 kDa. Band observed at ~130 kDa. |
靶标
-
功能
Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. -
序列相似性
Belongs to the rad21 family. -
结构域
The C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3. -
翻译后修饰
Cleaved by separase/ESPL1 at the onset of anaphase. Cleaved by caspase-3 and caspase-7 at the beginning of apoptosis. The cleavage by ESPL1 and caspase-3 take place at different sites.
Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK. -
细胞定位
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis. - Information by UniProt
-
数据库链接
- Entrez Gene: 540966 Cow
- Entrez Gene: 5885 Human
- Entrez Gene: 19357 Mouse
- Entrez Gene: 314949 Rat
- Omim: 606462 Human
- SwissProt: Q3SWX9 Cow
- SwissProt: O60216 Human
- SwissProt: Q61550 Mouse
see all -
别名
- CDLS4 antibody
- Double-strand-break repair protein rad21 homolog antibody
- hHR21 antibody
see all
图片
-
Western blot - Anti-Rad21 antibody (ab992)All lanes : Anti-Rad21 antibody (ab992) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : RKO (human cell line from colon carcinoma) whole cell lysate
Lane 5 : GaMG (human cell line from glioblastoma) whole cell lysate
Lane 6 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 7 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 8 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 9 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 10 : TCMK-1 (mouse epithelial kidney cell line transformed with SV40) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 72 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
-
Immunoprecipitation - Anti-Rad21 antibody (ab992)Immunoprecipitation analysis of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa (human epithelial cell line from cervix adenocarcinoma) cells prepared using NETN lysis buffer.
Lane 1: ab992 used for IP at 6 µg per reaction
Lane 2: Control IgG
For blotting immunoprecipitated Rad21, a rabbit anti-Rad21 recombinant monoclonal antibody was used at 1/1000. Detected using chemiluminescence with an exposure time of 3 minutes.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (186)
ab992 被引用在 186 文献中.
- Morgan SM et al. The three-dimensional structure of Epstein-Barr virus genome varies by latency type and is regulated by PARP1 enzymatic activity. Nat Commun 13:187 (2022). PubMed: 35039491
- Gines MM & Wendt KS A Robust Protocol for Investigating the Cohesin Complex by ChIP-Sequencing. Methods Mol Biol 2458:113-122 (2022). PubMed: 35103965
- Haarhuis JHI et al. A Mediator-cohesin axis controls heterochromatin domain formation. Nat Commun 13:754 (2022). PubMed: 35136067
- Luo H et al. HOTTIP-dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia. Mol Cell 82:833-851.e11 (2022). PubMed: 35180428
- Bedi YS et al. Alcohol induced increases in sperm Histone H3 lysine 4 trimethylation correlate with increased placental CTCF occupancy and altered developmental programming. Sci Rep 12:8839 (2022). PubMed: 35614060