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AB992

Anti-Rad21 抗体

Anti-Rad21 antibody

4

(20 Reviews)

|

(207 Publications)

Anti-Rad21 antibody (ab992) is a rabbit polyclonal antibody detecting Rad21 in Western Blot, IP. Suitable for Human, Mouse.

- Over 200 publications
- Trusted since 2002

查看别名

HR21, KIAA0078, NXP1, SCC1, RAD21, Double-strand-break repair protein rad21 homolog, hHR21, Nuclear matrix protein 1, SCC1 homolog, NXP-1

2 Images
Immunoprecipitation - Anti-Rad21 antibody (AB992)
  • IP

Supplier Data

Immunoprecipitation - Anti-Rad21 antibody (AB992)

Immunoprecipitation analysis of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa (human epithelial cell line from cervix adenocarcinoma) cells prepared using NETN lysis buffer.

Lane 1 : ab992 used for IP at 6 μg per reaction

Lane 2 : Control IgG

For blotting immunoprecipitated Rad21, a rabbit anti-Rad21 recombinant monoclonal antibody was used at 1/1000. Detected using chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-Rad21 antibody (ab992)

Predicted band size: 72 kDa

false

Western blot - Anti-Rad21 antibody (AB992)
  • WB

Supplier Data

Western blot - Anti-Rad21 antibody (AB992)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Rad21 antibody (ab992) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 4:

RKO (human cell line from colon carcinoma) whole cell lysate at 50 µg

Lane 5:

GaMG (human cell line from glioblastoma) whole cell lysate at 50 µg

Lane 6:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 50 µg

Lane 7:

A549 (human lung carcinoma cell line) whole cell lysate at 50 µg

Lane 8:

U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate at 50 µg

Lane 9:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Lane 10:

TCMK-1 (mouse epithelial kidney cell line transformed with SV40) whole cell lysate at 50 µg

Predicted band size: 72 kDa

true

Exposure time: 10s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human RAD21 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O60216

特异性

The epitope recognized by Anti-Rad21 antibody (ab992) maps to a region between residue 575 and the C-terminus (residue 631) human Rad21 homolog using the numbering given in entry NP_006256.1 (GeneID 5885).

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反应性数据

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您是否考虑以下替代产品？

AB217678

Anti-Rad21 antibody [EPR22506-15] - ChIP Grade

4
2 Reviews
查看产品
为什么推荐这个？

为向您提供最高品质的产品，我们在不断升级产品范围。我们选择了这个替代产品供您参考，因为它提供了更好的性能，更容易和更方便的使用，或交期更快。

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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Double-strand-break repair protein rad21 homolog. As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions (PubMed : 11509732). The cohesin complex may also play a role in spindle pole assembly during mitosis (PubMed : 11590136). In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome (By similarity). May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter (PubMed : 25575569). May play a role in embryonic gut development, possibly through the regulation of enteric neuron development (By similarity).. 64-kDa C-terminal product. May promote apoptosis.
See full target information RAD21
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文献 (207)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:8160 PubMed38071364

2023

Loss of cohesin regulator PDS5A reveals repressive role of Polycomb loops.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Bsteh,Hagar F Moussa,Georg Michlits,Ramesh Yelagandula,Jingkui Wang,Ulrich Elling,Oliver Bell

Nature communications 14:7947 PubMed38040722

2023

Defining a core configuration for human centromeres during mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ayantika Sen Gupta,Chris Seidel,Dai Tsuchiya,Sean McKinney,Zulin Yu,Sarah E Smith,Jay R Unruh,Jennifer L Gerton

Nature 623:183-192 PubMed37853125

2023

Chromatin compartmentalization regulates the response to DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Coline Arnould,Vincent Rocher,Florian Saur,Aldo S Bader,Fernando Muzzopappa,Sarah Collins,Emma Lesage,Benjamin Le Bozec,Nadine Puget,Thomas Clouaire,Thomas Mangeat,Raphael Mourad,Nadav Ahituv,Daan Noordermeer,Fabian Erdel,Martin Bushell,Aline Marnef,Gaëlle Legube

Nature communications 14:5615 PubMed37699887

2023

Multi-feature clustering of CTCF binding creates robustness for loop extrusion blocking and Topologically Associating Domain boundaries.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Hsin Chang,Sourav Ghosh,Andrea Papale,Jennifer M Luppino,Mélanie Miranda,Vincent Piras,Jéril Degrouard,Joanne Edouard,Mallory Poncelet,Nathan Lecouvreur,Sébastien Bloyer,Amélie Leforestier,Eric F Joyce,David Holcman,Daan Noordermeer

Nature genetics 55:1164-1175 PubMed37322110

2023

Sequential and directional insulation by conserved CTCF sites underlies the Hox timer in stembryos.

Applications

Unspecified application

Species

Unspecified reactive species

Hocine Rekaik,Lucille Lopez-Delisle,Aurélie Hintermann,Bénédicte Mascrez,Célia Bochaton,Alexandre Mayran,Denis Duboule

eLife 12: PubMed37010886

2023

Cohesin-independent STAG proteins interact with RNA and R-loops and promote complex loading.

Applications

Unspecified application

Species

Unspecified reactive species

Hayley Porter,Yang Li,Maria Victoria Neguembor,Manuel Beltran,Wazeer Varsally,Laura Martin,Manuel Tavares Cornejo,Dubravka Pezić,Amandeep Bhamra,Silvia Surinova,Richard G Jenner,Maria Pia Cosma,Suzana Hadjur

Science advances 9:eadg1123 PubMed37000871

2023

Light-activated macromolecular phase separation modulates transcription by reconfiguring chromatin interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Yoon Jung Kim,Michael Lee,Yi-Tsang Lee,Ji Jing,Jacob T Sanders,Giovanni A Botten,Lian He,Junhua Lyu,Yuannyu Zhang,Marcel Mettlen,Peter Ly,Yubin Zhou,Jian Xu

Molecular cell 83:1446-1461.e6 PubMed36996812

2023

Structural elements promote architectural stripe formation and facilitate ultra-long-range gene regulation at a human disease locus.

Applications

Unspecified application

Species

Unspecified reactive species

Liang-Fu Chen,Hannah Katherine Long,Minhee Park,Tomek Swigut,Alistair Nicol Boettiger,Joanna Wysocka

Nature microbiology 8:679-694 PubMed36959507

2023

SARS-CoV-2 restructures host chromatin architecture.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoyu Wang,Joo-Hyung Lee,Jieun Kim,Feng Xiong,Lana Al Hasani,Yuqiang Shi,Erin N Simpson,Xiaoyu Zhu,Yi-Ting Chen,Pooja Shivshankar,Joanna Krakowiak,Yanyu Wang,David M Gilbert,Xiaoyi Yuan,Holger K Eltzschig,Wenbo Li

Nature communications 14:1598 PubMed36949074

2023

A DNA tumor virus globally reprograms host 3D genome architecture to achieve immortal growth.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Wang,Xiang Liu,Jun Liang,Yohei Narita,Weiyue Ding,Difei Li,Luyao Zhang,Hongbo Wang,Merrin Man Long Leong,Isabella Hou,Catherine Gerdt,Chang Jiang,Qian Zhong,Zhonghui Tang,Carmy Forney,Leah Kottyan,Matthew T Weirauch,Benjamin E Gewurz,Mu-Sheng Zeng,Sizun Jiang,Mingxiang Teng,Bo Zhao
View all publications
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