AB992

Anti-Rad21 抗体

Name: Rad21抗体 (ab992)| Abcam中文官网
Brand: Abcam Limited
SKU: AB992
Price: 5267 CNY
Availability: InStock
Rating: 4 (20 reviews)

Anti-Rad21 antibody

(20 Reviews)

(249 Publications)

Anti-Rad21 antibody (ab992) is a rabbit polyclonal antibody detecting Rad21 in Western Blot, IP. Suitable for Human, Mouse.

- Over 200 publications
- Trusted since 2002

查看别名

HR21, KIAA0078, NXP1, SCC1, RAD21, Double-strand-break repair protein rad21 homolog, hHR21, Nuclear matrix protein 1, SCC1 homolog, NXP-1

2 Images

Supplier Data

Immunoprecipitation - Anti-Rad21 antibody (AB992)

Immunoprecipitation analysis of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa (human epithelial cell line from cervix adenocarcinoma) cells prepared using NETN lysis buffer.

Lane 1 : ab992 used for IP at 6 μg per reaction

Lane 2 : Control IgG

For blotting immunoprecipitated Rad21, a rabbit anti-Rad21 recombinant monoclonal antibody was used at 1/1000. Detected using chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-Rad21 antibody (ab992)

Predicted band size: 72 kDa

false

Supplier Data

Western blot - Anti-Rad21 antibody (AB992)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Rad21 antibody (ab992) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 4:

RKO (human cell line from colon carcinoma) whole cell lysate at 50 µg

Lane 5:

GaMG (human cell line from glioblastoma) whole cell lysate at 50 µg

Lane 6:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 50 µg

Lane 7:

A549 (human lung carcinoma cell line) whole cell lysate at 50 µg

Lane 8:

U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate at 50 µg

Lane 9:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Lane 10:

TCMK-1 (mouse epithelial kidney cell line transformed with SV40) whole cell lysate at 50 µg

Predicted band size: 72 kDa

true

Exposure time: 10s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human RAD21 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O60216

特异性

The epitope recognized by Anti-Rad21 antibody (ab992) maps to a region between residue 575 and the C-terminus (residue 631) human Rad21 homolog using the numbering given in entry NP_006256.1 (GeneID 5885).

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "ab992 used for IP at 6 μg per reaction.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "Band observed at ~130 kDa." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04 µg/mL", "WB-species-notes": "Band observed at ~130 kDa." }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Product Specifications
Anti-Rad21 antibody (ab992) is a rabbit polyclonal antibody and is validated for use in IP, WB in human, mouse samples.
Anti-Rad21 antibody (ab992) specifically detects Rad21 (UniProt ID: O60216; Molecular weight: 72kDa) and is sold in 50 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-Rad21 antibody (ab992) has high sensitivity and specificity.
Anti-Rad21 antibody (ab992) has been cited over 207 times in peer reviewed journals and is trusted by the scientific community.
Anti-Rad21 antibody (ab992) has 21 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone - ab251568.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Double-strand-break repair protein rad21 homolog. As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions (PubMed : 11509732). The cohesin complex may also play a role in spindle pole assembly during mitosis (PubMed : 11590136). In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome (By similarity). May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter (PubMed : 25575569). May play a role in embryonic gut development, possibly through the regulation of enteric neuron development (By similarity).. 64-kDa C-terminal product. May promote apoptosis.

See full target information RAD21

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文献 (249)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed41009406

2025

The Influence of Cohesin on the Short-Scale Dynamics of Intact and Damaged Chromatin in Different Phases of the Cell Cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Vladimir S Viushkov,Nikolai A Lomov,Polina O Kalitina,Daria M Potashnikova,Anastasia S Shtompel,Sergey V Ulianov,Sergey V Razin,Mikhail A Rubtsov

Epigenetics & chromatin 18:60 PubMed41013693

2025

Cohesin regulation of genome organization in mature granule neurons in the mouse cerebellum.

Applications

Unspecified application

Species

Unspecified reactive species

Omar A Payán Parra,Ziyu Zhao,Tomoko Yamada,Yue Yang

Oncogene 44:3609-3624 PubMed40764753

2025

MUC1-C auto-regulatory complex with EBNA1 is responsible for latent Epstein-Barr virus-associated gastric cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Ozawa,Yin Wang,Henry G Withers,Naoki Haratake,Ayako Nakashoji,Atrayee Bhattacharya,Atsushi Fushimi,Chie Kikutake,Kazuhiro Yamanoi,Shaowen White,Keyi Wang,Tatsuaki Daimon,Keisuke Shigeta,Kazumasa Fukuda,Hirofumi Kawakubo,Yuko Kitagawa,Mark D Long,Benjamin E Gewurz,Donald Kufe

Current biology : CB 35:3943-3958.e5 PubMed40730158

2025

Cohesin-mediated stabilization of the CCAN complex at kinetochores in mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Julian Haase,Koly Aktar,Mary Kate Bonner,Leonard Colin,Hindol Gupta,Briana E Marinoni,David O Morgan,Alexander E Kelly

Nature communications 16:6900 PubMed40715155

2025

SMCHD1 maintains heterochromatin, genome compartments and epigenome landscape in human myoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijun Huang,Wei Cui,Ishara Ratnayake,Kristin L Gallik,Lorna Cohen,Rabi Tawil,Gerd P Pfeifer

Epigenetics & chromatin 18:33 PubMed40490791

2025

Cohesin stabilization at promoters and enhancers by common transcription factors and chromatin regulators.

Applications

Unspecified application

Species

Unspecified reactive species

Audra F Bryan,Megan Justice,Alexis V Stutzman,Daniel J McKay,Jill M Dowen

Epigenetics & chromatin 18:31 PubMed40452075

2025

Unraveling the cohesin-chromatin interface: identifying protein interactions that modulate chromosome structure and function.

Applications

Unspecified application

Species

Unspecified reactive species

Natalie L Rittenhouse,Riya Gohil,June E Arricastres,Jill M Dowen

Genes 16: PubMed40282349

2025

A Super Enhancer-Derived Enhancer RNA Acts Together with CTCF/Cohesin in Trans to Regulate Erythropoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Bingning Xie,Ann Dean

Cell genomics 5:100813 PubMed40118069

2025

Binding domain mutations provide insight into CTCF's relationship with chromatin and its contribution to gene regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine Do,Guimei Jiang,Giulia Cova,Christos C Katsifis,Domenic N Narducci,Theodore Sakellaropoulos,Raphael Vidal,Priscillia Lhoumaud,Aristotelis Tsirigos,Faye Fara D Regis,Nata Kakabadze,Elphege P Nora,Marcus Noyes,Anders S Hansen,Jane A Skok

Nature communications 16:2148 PubMed40032827

2025

Interplay between CTCF-binding and CTCF-lacking regulatory elements in generating an architectural stripe at the Igh locus.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Ma,Noah Ollikainen,Hansen Du,Fatima Zohra Braikia,Nina Cui,Aisha Haley Bianchi,Christopher Dunn,Cuong Nguyen,Jinshui Fan,Supriyo De,Ranjan Sen,Xiang Qiu

View all publications

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