Anti-RAD18抗体(ab79763)
Key features and details
- Rabbit polyclonal to RAD18
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-RAD18抗体
参阅全部 RAD18 一抗 -
描述
兔多克隆抗体to RAD18 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
不与反应: Mouse -
免疫原
Recombinant fragment (GST-tag) corresponding to Human RAD18. GST at C-terminus 113 carboxyl terminal of human Rad18
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阳性对照
- A549 cells GM637 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79763于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 56 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 56 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the RAD18 family.
Contains 1 RING-type zinc finger.
Contains 1 SAP domain.
Contains 1 UBZ-type zinc finger. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 56852 Human
- Omim: 605256 Human
- SwissProt: Q9NS91 Human
- Unigene: 375684 Human
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别名
- 2810024C04Rik antibody
- DNA repair protein rad18 antibody
- E3 ubiquitin-protein ligase RAD18 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-RAD18 antibody (ab79763)Indirect immunofluorescence staining of GM637 cells . Note staining of nuclei.
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Western blot - Anti-RAD18 antibody (ab79763)Anti-RAD18 antibody (ab79763) at 1/1000 dilution + A549 crude extract
Predicted band size: 56 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
The lower thick band is native RAD18 and the upper thin band is mono-ubiquitinated RAD18 protein.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab79763 被引用在 6 文献中.
- Shimada M et al. Implication of E3 ligase RAD18 in UV-induced mutagenesis in human induced pluripotent stem cells and neuronal progenitor cells. J Radiat Res 64:345-351 (2023). PubMed: 36634340
- Wanzel M et al. CRISPR-Cas9-based target validation for p53-reactivating model compounds. Nat Chem Biol 12:22-8 (2016). WB . PubMed: 26595461
- Peng M et al. Crosstalk between BRCA-Fanconi anemia and mismatch repair pathways prevents MSH2-dependent aberrant DNA damage responses. EMBO J N/A:N/A (2014). WB ; Human . PubMed: 24966277
- Zeman MK et al. DNA damage-specific deubiquitination regulates Rad18 functions to suppress mutagenesis. J Cell Biol 206:183-97 (2014). Human . PubMed: 25023518
- Jones MJ et al. Dysregulation of DNA polymerase ? recruitment to replication forks results in genomic instability. EMBO J 31:908-18 (2012). WB ; Human . PubMed: 22157819
- Cotto-Rios XM et al. APC/CCdh1-dependent proteolysis of USP1 regulates the response to UV-mediated DNA damage. J Cell Biol 194:177-86 (2011). WB ; Human . PubMed: 21768287