AB168397

Anti-Rad1抗体

Name: Rad1抗体 (ab168397)| Abcam中文官网
Brand: Abcam Limited
SKU: AB168397
Price: 4606 CNY
Availability: InStock

Anti-Rad1 antibody

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(1 Publication)

Mouse Polyclonal RAD1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAD1.

查看别名

REC1, RAD1, Cell cycle checkpoint protein RAD1, hRAD1, Rad1-like DNA damage checkpoint protein

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Rad1 antibody (AB168397)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Rad1 antibody (AB168397)

Immunofluorescent analysis of HeLa cells labeling Rad1 with ab168397 at 10 µg/ml.

Unknown

Western blot - Anti-Rad1 antibody (AB168397)

All lanes:

Western blot - Anti-Rad1 antibody (ab168397) at 1 µg/mL

Lane 1:

Rad1 transfected 293T cell line lysate

Lane 2:

Non-transfected 293T cell line lysate

Predicted band size: 32 kDa

false

关键信息

宿主种属

Mouse

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

Recombinant Full Length Protein corresponding to Human RAD1.

O60671

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Rad1 also known as RAD1 homolog 1 from Saccharomyces cerevisiae functions mechanically as an exonuclease involved in DNA repair processes. It forms a part of the 9-1-1 checkpoint complex which acts as a sensor for DNA damage. Rad1 has a molecular mass of approximately 34 kDa and is expressed in various tissues where cell division occurs. Its primary role is in maintaining genomic stability by participating in the recognition and repair of abnormal DNA structures.

Biological function summary

Rad1 plays an essential role in the DNA damage response by being part of the 9-1-1 complex which includes Rad9 and Hus1 proteins. This complex is an important player in activating the ATR signaling pathway which helps cells respond to DNA replication stress and damage. Rad1 helps ensure proper checkpoint activation preventing cells with damaged DNA from progressing through the cell cycle which further safeguards genomic integrity.

Pathways

The protein Rad1 functions critically within the ATR-Chk1 signaling pathway a significant route for controlling cell cycle checkpoints. In this context Rad1 associates with proteins such as ATR and Chk1 facilitating the signaling necessary for cell cycle arrest under conditions of DNA stress. Rad1 also interacts with the base excision repair pathway where it works with other repair proteins to rectify oxidative DNA damage and maintain cellular viability.

Rad1 has a connection with cancer due to its involvement in DNA repair pathways. Impaired Rad1 function can lead to accumulation of DNA damage contributing to carcinogenesis. Additionally Rad1’s participation in maintaining genomic stability links it to disorders like Fanconi anemia where DNA repair defects are evident. In these contexts Rad1's interactions with proteins like BRCA1 further highlight its importance in protective cellular mechanisms.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

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Visit the Troubleshooting

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靶点信息

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair (PubMed : 10846170, PubMed : 10884395). The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex (PubMed : 12578958). Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER) (PubMed : 15871698). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates (PubMed : 15314187, PubMed : 15556996, PubMed : 15871698). The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase (PubMed : 21659603).

See full target information RAD1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:870 PubMed36797266

2023

Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2.

Applications

Unspecified application

Species

Unspecified reactive species

Karsten Nalbach,Martina Schifferer,Debjani Bhattacharya,Hung Ho-Xuan,Wei Chou Tseng,Luis A Williams,Alexandra Stolz,Stefan F Lichtenthaler,Zvulun Elazar,Christian Behrends

View all publications

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Anti-Rad1抗体

Anti-Rad1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Rad1 antibody (AB168397)

Western blot - Anti-Rad1 antibody (AB168397)