Anti-RACO-1抗体(ab272604)
Key features and details
- Rabbit polyclonal to RACO-1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RACO-1抗体 -
描述
兔多克隆抗体to RACO-1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Recombinant fragment corresponding to Human RACO-1 aa 150-250.
Database link: Q5TA31 -
阳性对照
- IHC-P: Human testis tissue. ICC/IF: U-2 OS.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272604于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
E3 ubiquitin-protein ligase that acts as a coactivator of JUN-mediated gene activation in response to growth factor signaling via the MAP3K1 pathway, independently from MAPK8. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 RING-type zinc finger. -
结构域
The RING-type zinc finger domain is required for E3 ligase activity. -
翻译后修饰
Ubiquitinated; undergoes 'Lys-48'-linked autoubiquitination in the absence of growth factors and MAP3K1-induced 'Lys-63'-linked polyubiquitination. 'Lys-48'-autoubiquitination leads to degradation by the proteasome, while MAP3K1-induced 'Lys-63'-linked polyubiquitination results in the stabilization of the protein. 'Lys-48'- and 'Lys-63'-linked polyubiquitinations occur most probably on the same 3 C-terminal lysine residues (Lys-195, Lys-223 and Lys-224) and are thus mutually exclusive. Other sites of ubiquitination are not excluded.
Arginine methylation by PRMT1 stabilizes RNF187 by facilitating K63-linked ubiquitin chain formation, and enables dimerization, c-Jun interaction and subsequent AP1 target gene expression. -
细胞定位
Cytoplasm. Nucleus. Predominantly located in the cytoplasm. Shuttles between the cytoplasm and the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 149603 Human
- Entrez Gene: 108660 Mouse
- Entrez Gene: 360533 Rat
- Omim: 613754 Human
- SwissProt: Q5TA31 Human
- SwissProt: Q8BFX1 Mouse
- SwissProt: D3Z8N2 Rat
- Unigene: 356377 Human
see all -
别名
- E3 ubiquitin-protein ligase RNF187 antibody
- Protein RNF187 antibody
- RACO-1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACO-1 antibody (ab272604)
Paraffin-embedded formalin-fixed Human testis tissue stained for RACO-1 using ab272604 at 1/200 in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-RACO-1 antibody (ab272604)PFA fixed, Triton X-100 permeablized human cell line U-2 labeling RACO-1 (green) using ab272604 at 2 ug/ml in ICC/IF analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272604 尚未被引用在任何文献中。