重组RACK1抗体[EPR7388](ab129084)|Abcam中文官网

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7388] to RACK1
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Carrier Free

产品名称

Anti-RACK1抗体[EPR7388]
参阅全部 RACK1 一抗
描述

兔单克隆抗体[EPR7388] to RACK1
宿主

Rabbit
经测试应用

适用于: WB, IHC-Pmore details
不适用于: ICC/IF or IP
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: HeLa, HepG2, SH-SY5Y, MCF7, C6, PC-12, Mouse brain, and NIH/3T3 lysates. IHC-P: human colon carcinoma, and cervical carcinoma, mouse cerebrum, and rat cerebrum tissues.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR7388
同种型

IgG
研究领域

Alternative Versions
- Anti-RACK1 antibody [EPR7388] - BSA and Azide free (ab248289)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- MCF7 membrane extract lysate (ab29539)
- HeLa membrane extract lysate (ab29547)
Recombinant Protein
- Recombinant Human RACK1 protein (ab119442)

The Abpromise guarantee

Abpromise™承诺保证使用ab129084于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB		1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa).
IHC-P		1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50 -1/100

说明
WB 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50 -1/100

应用说明

Is unsuitable for ICC/IF or IP.

功能

Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
序列相似性

Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats.
结构域

The WD repeats domain 5 mediates interaction with TRIM63.
细胞定位

Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
Target information above from: UniProt accession P63244 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 10399 Human
- Entrez Gene: 14694 Mouse
- Entrez Gene: 83427 Rat
- Omim: 176981 Human
- SwissProt: P63244 Human
- SwissProt: P68040 Mouse
- SwissProt: P63245 Rat
- Unigene: 5662 Human
- Unigene: 5305 Mouse
- Unigene: 55487 Rat
see all
别名
- Cell proliferation-inducing gene 21 protein antibody
- GBLP_HUMAN antibody
- Gnb2-rs1 antibody
- Gnb2l1 antibody
- Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 antibody
- Guanine nucleotide binding protein beta polypeptide 2 like 1 antibody
- Guanine nucleotide binding protein beta subunit 2 like 1 antibody
- Guanine nucleotide binding protein beta subunit like protein 12.3 antibody
- Guanine nucleotide binding protein subunit beta 2 like 1 antibody
- Guanine nucleotide binding protein subunit beta like protein 12.3 antibody
- Guanine nucleotide-binding protein subunit beta-2-like 1 antibody
- Guanine nucleotide-binding protein subunit beta-like protein 12.3 antibody
- H12.3 antibody
- HLC-7 antibody
- Human lung cancer oncogene 7 protein antibody
- lung cancer oncogene 7 antibody
- OTTHUMP00000223704 antibody
- OTTHUMP00000223870 antibody
- OTTHUMP00000223891 antibody
- OTTHUMP00000223893 antibody
- OTTHUMP00000223900 antibody
- OTTHUMP00000223902 antibody
- OTTHUMP00000223930 antibody
- OTTHUMP00000223931 antibody
- PIG21 antibody
- Proliferation inducing gene 21 antibody
- Protein homologous to chicken B complex protein guanine nucleotide binding antibody
- RACK1 antibody
- Receptor for activated C kinase 1 antibody
- Receptor for activated C kinase antibody
- Receptor of activated protein kinase C 1 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling RACK1 with Purified ab129084 at 1:1000 dilution (0.201 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling RACK1 with Purified ab129084 at 1:1000 dilution (0.201 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling RACK1 with Purified ab129084 at 1:1000 dilution (0.201 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-RACK1 antibody [EPR7388] (ab129084)

All lanes : Anti-RACK1 antibody [EPR7388] (ab129084) at 1/1000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 6 : Mouse brain lysates
Lane 7 : Mouse spleen lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 35 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Western blot - Anti-RACK1 antibody [EPR7388] (ab129084)

All lanes : Anti-RACK1 antibody [EPR7388] (ab129084) at 1/1000 dilution

Lane 1 : MCF7 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : PC12 cell lysate
Lane 5 : C6 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)

ab129084, at a dilution of 1/50, staining RACK1 in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-RACK1 antibody [EPR7388] (ab129084)

Flow cytometry protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

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发表研究结果有使用 ab129084？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab129084 被引用在 7 文献中.

Wu J et al. EST12 regulates Myc expression and enhances anti-mycobacterial inflammatory response via RACK1-JNK-AP1-Myc immune pathway. Front Immunol 13:943174 (2022). PubMed: 36003390
Zhang X et al. CPNE3 interaction with RACK1 protects against myocardial ischemia/reperfusion injury. Exp Ther Med 23:128 (2022). PubMed: 34970351
Filho EGF et al. RACK1 plays a critical role in mast cell secretion and Ca2+ mobilization by modulating F-actin dynamics. J Cell Sci 134:N/A (2021). PubMed: 34550354
Dan H et al. RACK1 promotes cancer progression by increasing the M2/M1 macrophage ratio via the NF-?B pathway in oral squamous cell carcinoma. Mol Oncol 14:795-807 (2020). PubMed: 31997535
Li XY et al. RACK1 Acts as a Potential Tumor Promoter in Colorectal Cancer. Gastroenterol Res Pract 2019:5625026 (2019). PubMed: 30962803
Kuppusamy M et al. Interaction of the Mineralocorticoid Receptor With RACK1 and Its Role in Aldosterone Signaling. Endocrinology 158:2367-2375 (2017). PubMed: 28472300
Corcoran KA et al. Regulation of fear extinction versus other affective behaviors by discrete cortical scaffolding complexes associated with NR2B and PKA signaling. Transl Psychiatry 5:e657 (2015). PubMed: 26460481

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

序列相似性

结构域

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (7)

客户评价及客户问答