重组Anti-RACK1抗体[EPR7388]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (AB129084)

ab129084, at a dilution of 1/50, staining RACK1 in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (AB129084)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling RACK1 with Purified ab129084 at 1 : 1000 dilution (0.201 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (AB129084)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling RACK1 with Purified ab129084 at 1 : 1000 dilution (0.201 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (AB129084)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling RACK1 with Purified ab129084 at 1 : 1000 dilution (0.201 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• WB

Lab

Western blot - Anti-RACK1 antibody [EPR7388] (AB129084)

All lanes:

Western blot - Anti-RACK1 antibody [EPR7388] (ab129084) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 3:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 4:

C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 20 µg

Lane 6:

Mouse brain lysates at 20 µg

Lane 7:

Mouse spleen lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 35 kDa

Observed band size: 32 kDa

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• WB

Unknown

Western blot - Anti-RACK1 antibody [EPR7388] (AB129084)

All lanes:

Western blot - Anti-RACK1 antibody [EPR7388] (ab129084) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

PC12 cell lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 35 kDa

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• WB

CiteAb

Western blot - Anti-RACK1 antibody [EPR7388] (AB129084)

RACK1 western blot using anti-RACK1 antibody [EPR7388] ab129084. Publication image and figure legend from Dan, H., Liu, S., et al., 2020, Mol Oncol, PubMed 31997535.

ab129084 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab129084 please see the product overview.

RACK1 increases the M2/M1 ratio in an NF-κB axis-dependent manner. (A) Human cytokine array analysis of the conditioned medium from HSC-3 control cells and HSC-3 cells with RACK1 silencing for 36 h. (B) Unsupervised hierarchical clustering analysis by complete linkage between si-NC- and si-RACK1-transfected HSC-3 cells for 36 h. (C) Analysis of CCL5, CSF2, CXCL10, IL-8 and CXCL2 mRNA levels in HSC-3 cells transfected with si-NC- or si-RACK1 for 36 h (p < 0.05). (D) Western blots for p-NF-κB and RACK1 in two infected OSCC cell lines. (E) Macrophages were induced from THP-1 cells by PMA and cocultured with sh-NC HSC-3 cell supernatant (with DMSO or BAY 11-7082 included) for 8 h. After centrifugation, the macrophages were stained with CD206 and CCR7 antibodies. The percentages and cell numbers of macrophages were analyzed using flow cytometry. (F) Analysis of the M2/M1 ratio in different induction groups (DMSO or BAY 11-7082) (mean ± SD; *p < 0.05).

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