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Anti-RACK1抗体(ab62735)

Reviews (3)Q&A (1)References (11)
Western blot - Anti-RACK1 antibody (ab62735)

    Key features and details

    • Rabbit polyclonal to RACK1
    • Suitable for: ICC/IF, WB, IP
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-RACK1 antibody [EPR7388] (ab129084)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-RACK1抗体
      参阅全部 RACK1 一抗

    • 描述

      兔多克隆抗体to RACK1

    • 宿主

      Rabbit

    • 经测试应用

      适用于: ICC/IF, WB, IPmore details

    • 种属反应性

      与反应: Mouse, Rat, Human
      预测可用于: Chicken, Cow, Pig, Xenopus laevis, Cynomolgus monkey

    • 免疫原

      Synthetic peptide:

      TEQMTLRGT LKGHNG - C

      , conjugated to KLH by a C terminal Cysteine residue linker, corresponding to N terminal amino acids 2-16 of Human RACK1
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab62735于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC/IF
    Use at an assay dependent concentration.
    WB (2)
    1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 35 kDa).
    IP (1)
    Use at an assay dependent concentration.

    Use 5-10 µL.
    说明
    ICC/IF
    Use at an assay dependent concentration.
    WB
    1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 35 kDa).
    IP
    Use at an assay dependent concentration.

    Use 5-10 µL.

    靶标

    • 功能

      Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.

    • 序列相似性

      Belongs to the WD repeat G protein beta family.
      Contains 7 WD repeats.

    • 结构域

      The WD repeats domain 5 mediates interaction with TRIM63.

    • 细胞定位

      Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
    • Information by UniProt

    • 数据库链接

      see all

    • 别名

      • Cell proliferation-inducing gene 21 protein antibody
      • GBLP_HUMAN antibody
      • Gnb2-rs1 antibody
      • Gnb2l1 antibody
      • Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 antibody
      • Guanine nucleotide binding protein beta polypeptide 2 like 1 antibody
      • Guanine nucleotide binding protein beta subunit 2 like 1 antibody
      • Guanine nucleotide binding protein beta subunit like protein 12.3 antibody
      • Guanine nucleotide binding protein subunit beta 2 like 1 antibody
      • Guanine nucleotide binding protein subunit beta like protein 12.3 antibody
      • Guanine nucleotide-binding protein subunit beta-2-like 1 antibody
      • Guanine nucleotide-binding protein subunit beta-like protein 12.3 antibody
      • H12.3 antibody
      • HLC-7 antibody
      • Human lung cancer oncogene 7 protein antibody
      • lung cancer oncogene 7 antibody
      • OTTHUMP00000223704 antibody
      • OTTHUMP00000223870 antibody
      • OTTHUMP00000223891 antibody
      • OTTHUMP00000223893 antibody
      • OTTHUMP00000223900 antibody
      • OTTHUMP00000223902 antibody
      • OTTHUMP00000223930 antibody
      • OTTHUMP00000223931 antibody
      • PIG21 antibody
      • Proliferation inducing gene 21 antibody
      • Protein homologous to chicken B complex protein guanine nucleotide binding antibody
      • RACK1 antibody
      • Receptor for activated C kinase 1 antibody
      • Receptor for activated C kinase antibody
      • Receptor of activated protein kinase C 1 antibody
      see all

    图片

    • Western blot - Anti-RACK1 antibody (ab62735)
      Western blot - Anti-RACK1 antibody (ab62735)
      All lanes : Anti-RACK1 antibody (ab62735) at 1/500 dilution

      Lane 1 : Extract from hek-293T cell line
      Lane 2 : Extract from HeLa cell line
      Lane 3 : Extract from MCF7 cell line
      Lane 4 : Extract from U2OS cell line
      Lane 5 : Extract from Jurkat cell line
      Lane 6 : Extract from RAT1 cell line
      Lane 7 : Extract from PC12 cell line
      Lane 8 : Extract from NIH3T3 cell line

      Secondary
      All lanes : Goat Anti-Rabbit IgG

      Developed using the ECL technique.

      Predicted band size: 35 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?

    文献 (11)

    发表研究结果有使用 ab62735？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab62735 被引用在 11 文献中.

    • Wan L  et al. Translation stress and collided ribosomes are co-activators of cGAS. Mol Cell 81:2808-2822.e10 (2021). PubMed: 34111399
    • Li H  et al. Repetitive Transcranial Magnetic Stimulation Alleviates Neurological Deficits After Cerebral Ischemia Through Interaction Between RACK1 and BDNF exon IV by the Phosphorylation-Dependent Factor MeCP2. Neurotherapeutics 17:651-663 (2020). PubMed: 31912469
    • Chen Y  et al. O-GlcNAcylated c-Jun antagonizes ferroptosis via inhibiting GSH synthesis in liver cancer. Cell Signal 63:109384 (2019). PubMed: 31394193
    • Zhu L  et al. Upregulated RACK1 attenuates gastric cancer cell growth and epithelial-mesenchymal transition via suppressing Wnt/ß-catenin signaling. Onco Targets Ther 12:4795-4805 (2019). PubMed: 31417279
    • Lee JS  et al. RACK1 mediates rewiring of intracellular networks induced by hepatitis C virus infection. PLoS Pathog 15:e1008021 (2019). PubMed: 31525236
    View all Publications for this product

    客户评价及客户问答

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    1-4 of 4 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-RACK1 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (colon cancer)
    Total protein in input
    1000 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    colon cancer
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 19 2020

    Western blot abreview for Anti-RACK1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293t)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    60 µg
    Treatment
    mmc 2h
    Specification
    293t
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 31 2018

    Western blot abreview for Anti-RACK1 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (BMM)
    Loading amount
    15 µg
    Specification
    BMM
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 13 2010

    Question

    I am very interested in your RACK1 antibody (ab62735). I would like use this antibody to do immunofluorescence on primary mouse cortical neurons. However, I do not find any papers citing the use of this antibody in any type of primary neurons. Would it be possible for me to get a small sample of this antibody to try before purchasing?

    Read More
    Answer

    Thank you very much for your interest in ab62735.

    We would guarantee ab62735 to work in ICC/IF with mouse cells. We have this reference citing previous use for ICC/IF (though not in mouse primary neurons). http://www.ncbi.nlm.nih.gov/pubmed/22210188?dopt=Abstract

    We do not currently have the ability to store multiple vial sizes or trial sizes of our products.

    However by participating in our AbTrial program you can now use our products that do not have images of your application without financial risk.

    Simply follow these easy steps below to apply for our AbTrial Program:

    1. Reply to this email, letting us know you are interested in testing this product in ICC/IF and submitting an image of your results to us.

    2. Our scientists will email you an inactive personal discount code for the value of the product.

    3. Purchase and test the product at the regular price.

    4. Submit an image of your results, including your discount code in the additional notes section of your Abreview.

    5. Once the Abreview is submitted, the discount code will become active.

    6. Apply your discount code on your next order to receive that value off.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

    I hope this information helps. Please let us know if you have any other questions or if you would like to enroll in the AbTrial program.

    Read More

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