Anti-RACGAP1/MGCRACGAP抗体(ab2270)
Key features and details
- Goat polyclonal to RACGAP1/MGCRACGAP
- Suitable for: IHC-P, ICC, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RACGAP1/MGCRACGAP抗体
参阅全部 RACGAP1/MGCRACGAP 一抗 -
描述
山羊多克隆抗体to RACGAP1/MGCRACGAP -
宿主
Goat -
经测试应用
适用于: IHC-P, ICC, WBmore details -
种属反应性
与反应: Human
预测可用于: Cow, Dog
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免疫原
Synthetic peptide corresponding to Human RACGAP1/MGCRACGAP aa 600 to the C-terminus (C terminal). (NP_001306934.1; NP_001306935.1; NP_001306936.1)
Database link: Q9H0H5 -
阳性对照
- WB: Human testis tissue lysate. K562 and Jurkat cell lysates. ICC: MCF7 cells. IHC-P: Human testis tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 1 µg/ml.
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| ICC |
Use a concentration of 10 µg/ml.
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| WB | (2) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 71 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
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IHC-P
Use a concentration of 1 µg/ml. |
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ICC
Use a concentration of 10 µg/ml. |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 71 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells. -
组织特异性
Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase. -
序列相似性
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Rho-GAP domain. -
结构域
The coiled coil region is indispensible for localization to the midbody during cytokinesis. -
翻译后修饰
Phosphorylated at multiple sites in the midbody during cytokinesis. Phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA. Phosphorylation on multiple serine residues by PLK1 enhances its association with ECT2 and is critical for cleavage furrow formation. -
细胞定位
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Cytoplasmic vesicle > secretory vesicle > acrosome. Cleavage furrow. Midbody. Colocalizes with RND2 in Golgi-derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase. - Information by UniProt
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数据库链接
- Entrez Gene: 29127 Human
- Omim: 604980 Human
- SwissProt: Q9H0H5 Human
- Unigene: 505469 Human
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别名
- CYK4 antibody
- GAP antibody
- Gap1 antibody
see all
图片
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Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab2270)All lanes :
Lane 1 : K562 cell lysate (in RIPA buffer)
Lane 2 : Jurkat cell lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 71 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACGAP1/MGCRACGAP antibody (ab2270)Paraffin embedded human testis tissue stained for RACGAP1/MGCRACGAP using ab2270 at 4 µg/ml in immunohistochemical analysis.
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Immunocytochemistry - Anti-RACGAP1/MGCRACGAP antibody (ab2270)Immunoctochemistry analysis of paraformaldehyde fixed MCF7 (human breast adenocarcinoma cell line) cells labeling RACGAP1/MGCRACGAP with ab2270 at 10 µg/mL. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour followed by Alexa Fluor® 488 secondary antibody (4 µg/mL). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (4 µg/mL). -
Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab2270)Anti-RACGAP1/MGCRACGAP antibody (ab2270) at 0.2 µg/ml + Human Testis lysate (RIPA buffer) at 30 µg
Predicted band size: 71 kDaab2270 staining (0.2µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab2270)Anti-RACGAP1/MGCRACGAP antibody (ab2270) at 1 µg/ml + K562 cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 71 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (25)
ab2270 被引用在 25 文献中.
- Pust S et al. Vesicle-mediated transport of ALIX and ESCRT-III to the intercellular bridge during cytokinesis. Cell Mol Life Sci 80:235 (2023). PubMed: 37523003
- Jiang H et al. Human Endonuclease ANKLE1 Localizes at the Midbody and Processes Chromatin Bridges to Prevent DNA Damage and cGAS-STING Activation. Adv Sci (Weinh) 10:e2204388 (2023). PubMed: 36825683
- Khalid M et al. Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma: The Clinical Significance of Direct RACGAP1 Regulation. Cancers (Basel) 11:N/A (2019). PubMed: 30866526
- Pu J et al. lncRNA MAGI2-AS3 Prevents the Development of HCC via Recruiting KDM1A and Promoting H3K4me2 Demethylation of the RACGAP1 Promoter. Mol Ther Nucleic Acids 18:351-362 (2019). PubMed: 31629962
- Adriaans IE et al. PLK1 plays dual roles in centralspindlin regulation during cytokinesis. J Cell Biol 218:1250-1264 (2019). PubMed: 30728176
