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Cell Biology Cell Cycle Kinases/Phosphatases Other

Anti-RAC1 + Cdc42 (phospho S71)抗体(ab5482)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (7)

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Western blot - Anti-RAC1 + Cdc42 (phospho S71) antibody (ab5482)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC1 + Cdc42 (phospho S71) antibody (ab5482)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC1 + Cdc42 (phospho S71) antibody (ab5482)

Key features and details

  • Rabbit polyclonal to RAC1 + Cdc42 (phospho S71)
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-RAC1 + Cdc42 (phospho S71)抗体
    参阅全部 RAC1 + Cdc42 一抗
  • 描述

    兔多克隆抗体to RAC1 + Cdc42 (phospho S71)
  • 宿主

    Rabbit
  • 特异性

    Cdc 42 [pS71] (100% homologous) and Rho A/B/C [pS73] (92% homologous) are expected to react.
  • 经测试应用

    适用于: IHC-P, WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human RAC1 + Cdc42 (phospho S71). The sequence is conserved in human and mouse RAC 1, 2, and 3, and Cdc 42 human, mouse, rat, dog and frog.

  • 阳性对照

    • WB: A431 cells treated with EGF. IHC-P: human skin, human stomach tissues
  • 常规说明


    RAC, Cdc 42 and Rho A, B, and C are members of a small RhoGTPase family that bind and hydrolyze GTP. GTP bound RAC 1 and cdc 42 play a pivotal role in controlling cell shape, adhesion, growth and transformation. Active Rac 1 is implicated in regulating serum response element (SRE), NFAT 1 and nuclear factor kappa B (NF kappa B) transcription activities. Activated RAC 1 and Cdc 42 bind and activate PAK 1, which in turn activates key downstream signaling proteins including MEKK 1 and JNK. RAC 1 and Cdc 42 are phosphorylated on serine 71, a putative Akt site located between the protein binding domain and GTP binding domain. Phosphorylation of RAC 1 on serine 71 regulates its GTP binding and GTPase activity.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated RAC 1. The final product is generated by affinity chromatography using a RAC 1 derived peptide that is phosphorylated at serine 71.
  • Primary antibody说明

    RAC, Cdc 42 and Rho A, B, and C are members of a small RhoGTPase family that bind and hydrolyze GTP. GTP bound RAC 1 and cdc 42 play a pivotal role in controlling cell shape, adhesion, growth and transformation. Active Rac 1 is implicated in regulating serum response element (SRE), NFAT 1 and nuclear factor kappa B (NF kappa B) transcription activities. Activated RAC 1 and Cdc 42 bind and activate PAK 1, which in turn activates key downstream signaling proteins including MEKK 1 and JNK. RAC 1 and Cdc 42 are phosphorylated on serine 71, a putative Akt site located between the protein binding domain and GTP binding domain. Phosphorylation of RAC 1 on serine 71 regulates its GTP binding and GTPase activity.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A-431 whole cell lysate (ab7909)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5482于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/20 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (1)
1/1000. Detects a band of approximately 23 kDa.
说明
IHC-P
1/20 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 23 kDa.

靶标

  • 相关性

    Cdc42/Rac belongs to the superfamily of small GTPases that are structurally linked to the proto-oncogene product p21ras and are important for the control of cell growth and differentiation as well as for intracellular organization. Cdc42/Rac is an important upstream regulator of the protein kinase cascade that controls the SAPK/JNK and p38 activity. Recent data also suggest that constitutive active forms of Cdc42 can induce apoptosis through a mechanism requiring signaling through SAPK/JNK.
  • 细胞定位

    Cell membrane; Lipid anchor; Cytoplasmic side.
  • 数据库链接

    • Entrez Gene: 5879 Human
    • Entrez Gene: 998 Human
    • Omim: 116952 Human
    • Omim: 602048 Human
    • SwissProt: P60953 Human
    • SwissProt: P63000 Human
    • 别名

      • CDC42Hs antibody
      • Cell division cycle 42 antibody
      • G25K antibody
      • MIG5 antibody
      • Migration inducing gene 5 antibody
      • p21 Rac1 antibody
      • Ras like protein TC25 antibody
      • Small GTP binding protein Cdc42 antibody
      • TC25 antibody
      see all

    图片

    • Western blot - Anti-RAC1 + Cdc42 (phospho S71) antibody (ab5482)
      Western blot - Anti-RAC1 + Cdc42 (phospho S71) antibody (ab5482)
      Peptide Competition and Phosphatase Treatment:
      Lysates prepared from A431 cells left unstimulated (1) or stimulated with EGF (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either not treated (1-5) or treated with lambda phosphatase (6), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab5482 antibody for two hours at room temperature in a 3% BSATBST buffer, following prior incubation with: no peptide (1, 2, 6), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphoserine containing peptide (4), or, the phosphopeptide immunogen (5), After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that the peptide corresponding to ab5482 blocks the antibody signal. The data also shows that phosphatase stripping eliminates the signal, verifying that the antibody is pho
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC1 + Cdc42 (phospho S71) antibody (ab5482)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC1 + Cdc42 (phospho S71) antibody (ab5482)

      Immunohistochemiscal analysis of paraffin-embedded human skin tissue labeling RAC1 + Cdc42 (phospho S71) with ab5482 at 1/100 dilution (right) compared to a negative control without primary antibody (left).

      To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab5482 diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC1 + Cdc42 (phospho S71) antibody (ab5482)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC1 + Cdc42 (phospho S71) antibody (ab5482)

      Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling RAC1 + Cdc42 (phospho S71) with ab5482 at 1/20 dilution (right) compared to a negative control without primary antibody (left).

      To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab5482 diluted in 3% BSA-PBS at a dilution of 1/20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    实验方案

    • Peptide Competition Experiment
    • Western blotting protocol

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (7)

    发表研究结果有使用 ab5482?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5482 被引用在 7 文献中.

    • Li N  et al. LPM580098, a Novel Triple Reuptake Inhibitor of Serotonin, Noradrenaline, and Dopamine, Attenuates Neuropathic Pain. Front Pharmacol 10:53 (2019). PubMed: 30837867
    • Kang DS  et al. Netrin-1/DCC-mediated PLC?1 activation is required for axon guidance and brain structure development. EMBO Rep 19:N/A (2018). PubMed: 30224412
    • Giralt A  et al. Pyk2 is essential for astrocytes mobility following brain lesion. Glia 64:620-34 (2016). PubMed: 26663135
    • Maurya VK  et al. Expression and activity of Rac1 is negatively affected in the dehydroepiandrosterone induced polycystic ovary of mouse. J Ovarian Res 7:32 (2014). PubMed: 24628852
    • Haas S  et al. DIGE proteome analysis reveals suitability of ischemic cardiac in vitro model for studying cellular response to acute ischemia and regeneration. PLoS One 7:e31669 (2012). WB ; Mouse . PubMed: 22384053
    • Pickl M & Ries CH Comparison of 3D and 2D tumor models reveals enhanced HER2 activation in 3D associated with an increased response to trastuzumab. Oncogene 28:461-8 (2009). WB ; Human . PubMed: 18978815
    • Tan AM  et al. Neuropathic pain memory is maintained by Rac1-regulated dendritic spine remodeling after spinal cord injury. J Neurosci 28:13173-83 (2008). WB ; Rat . PubMed: 19052208

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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-RAC1 + Cdc42 (phospho S71) antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    30 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 16 2017

    Question

    What is the IgG concentration? The lot number is GR67743.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 27 2013

    Answer

    The antibody concentration of the lot you have is 0.35 mg/ml.

    Read More

    Abcam Scientific Support

    回复于 Feb 27 2013

    Question

    I have a question regarding your Rac/Cdc42 antibody: As mentioned in the description for your RAC antibody [23A8], rac shows a band at 21 kDa, while the CDC42 antibody (ab14241)shows a band at 24 kDa. Does the Rac/Cdc42 antibody (a5482) that recognizes both proteins display two seperate bands which would allow to quantify the the amount of Cdc42 and Rac in one blot or is it just one broad band? Thanks for your help

    Read More

    Abcam community

    Verified customer

    Asked on Sep 30 2004

    Answer

    This antibody recognizes a sequence that is exactly conserved amongst the proteins. We would thus expect that providing you resolve your gel in order to separate the two proteins, the antibody should recognise the two proteins separately.

    Read More

    Abcam Scientific Support

    回复于 Oct 04 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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