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Neuroscience Neurotransmission Secretory Vesicles Rabs

Anti-Rabphilin 3A抗体(ab3338)

  • Datasheet
Reviews (1) Submit a question References (3)

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Western blot - Anti-Rabphilin 3A antibody (ab3338)
  • Western blot - Anti-Rabphilin 3A antibody (ab3338)
  • Western blot - Anti-Rabphilin 3A antibody (ab3338)
  • Immunocytochemistry/ Immunofluorescence - Anti-Rabphilin 3A antibody (ab3338)

Key features and details

  • Rabbit polyclonal to Rabphilin 3A
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Rabphilin 3A抗体
    参阅全部 Rabphilin 3A 一抗
  • 描述

    兔多克隆抗体to Rabphilin 3A
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Rat Rabphilin 3A aa 1-18.
    Sequence:

    MTDTVVNRWMYPGDGPLQ


    (Peptide available as ab4954)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: Rat Brain ; Mouse Brain ICC/IF: RSC96
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Rabs
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3338于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
Use a concentration of 1 µg/ml.

By Western blot, this antibody detects an ~75 kDa protein representing Rabphilin 3A from rat brain whole protein extract.

ICC/IF
Use a concentration of 2 µg/ml.
说明
WB
Use a concentration of 1 µg/ml.

By Western blot, this antibody detects an ~75 kDa protein representing Rabphilin 3A from rat brain whole protein extract.

ICC/IF
Use a concentration of 2 µg/ml.

靶标

  • 功能

    Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
  • 序列相似性

    Contains 2 C2 domains.
    Contains 1 FYVE-type zinc finger.
    Contains 1 RabBD (Rab-binding) domain.
  • 细胞定位

    Cell junction > synapse.
  • Target information above from: UniProt accession Q9Y2J0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 19894 Mouse
    • Entrez Gene: 171039 Rat
    • SwissProt: P47708 Mouse
    • SwissProt: P47709 Rat
    • Unigene: 181166 Mouse
    • Unigene: 10976 Rat
    • 别名

      • Exophilin 1 antibody
      • Exophilin-1 antibody
      • Exophilin1 antibody
      • KIAA0985 antibody
      • Rabphilin 3A homolog antibody
      • Rabphilin-3A antibody
      • Rabphilin3A antibody
      • RP3A antibody
      • RP3A_HUMAN antibody
      • Rph 3a antibody
      • Rph3a antibody
      see all

    图片

    • Western blot - Anti-Rabphilin 3A antibody (ab3338)
      Western blot - Anti-Rabphilin 3A antibody (ab3338)
      All lanes : Anti-Rabphilin 3A antibody (ab3338) at 3 µg/ml

      Lane 1 : Mouse Brain
      Lane 2 : Rat Brain
      Lane 3 : Rat Lung
      Lane 4 : Rat Liver
      Lane 5 : Rat Kidney

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP conjugated Goat anti-Rabbit IgG at 1/4000 dilution

      Additional bands at: ~76 kDa. We are unsure as to the identity of these extra bands.



      Detection: chemiluminescence

      Rabphilin 3A observed in Mouse and Rat Brain, and not observed in other tissues which are documented to be Rabphilin 3A negative. 

    • Western blot - Anti-Rabphilin 3A antibody (ab3338)
      Western blot - Anti-Rabphilin 3A antibody (ab3338)
      Anti-Rabphilin 3A antibody (ab3338) at 2 µg/ml + Rat Brain

      Secondary
      HRP conjugated Goat anti-Rabbit IgG at 1/2500 dilution

      Additional bands at: ~76 kDa. We are unsure as to the identity of these extra bands.



      Detection: chemiluminescence

    • Western blot - Anti-Rabphilin 3A antibody (ab3338)
      Western blot - Anti-Rabphilin 3A antibody (ab3338)

      Western blot detection of Rabphilin 3A in rat brain extract using ab3338.

    • Immunocytochemistry/ Immunofluorescence - Anti-Rabphilin 3A antibody (ab3338)
      Immunocytochemistry/ Immunofluorescence - Anti-Rabphilin 3A antibody (ab3338)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton™ X-100 permeabilized, 70% confluent log phase RSC96 (rat neuronal Schwann cell) cells, blocked with 3% BSA-PBS for 1 hour at room temperature. Labelling Rabphilin 3A with ab3338 at 2 µg/mL and incubated for 3 hours at room temperature then labelled with secondary antibody goat anti-rabbit igG, Alexa Fluor® 488 conjugate at a 1/2000 dilution for 45 minutes at room temperature (Green). Nuclei stained with DAPI (Blue). F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin at a dilution of 1/300 (Red). Panel e shows the no primary antibody control. The images were captured at 60X magnification.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (3)

    发表研究结果有使用 ab3338?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3338 被引用在 3 文献中.

    • Selma-Soriano E  et al. Rabphilin silencing causes dilated cardiomyopathy in a Drosophila model of nephrocyte damage. Sci Rep 11:15287 (2021). PubMed: 34315987
    • Selma-Soriano E  et al. Rabphilin involvement in filtration and molecular uptake in Drosophila nephrocytes suggests a similar role in human podocytes. Dis Model Mech 13:N/A (2020). PubMed: 32680845
    • Xu XQ  et al. S1PR1 mediates anti-apoptotic/pro-proliferative processes in human acute myeloid leukemia cells. Mol Med Rep 14:3369-75 (2016). PubMed: 27572094

    客户评价及客户问答

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    Western blot abreview for Anti-Rabphilin 3A antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    5 µg
    Gel Running Conditions
    Non-reduced Denaturing
    Sample
    Rat Tissue lysate - whole (hippocampus tissue lysate)
    Specification
    hippocampus tissue lysate
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 07 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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