Anti-RABEP1抗体(ab176578)
Key features and details
- Rabbit polyclonal to RABEP1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-RABEP1抗体 -
描述
兔多克隆抗体to RABEP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Chicken, Cow, Dog, Chimpanzee, Rhesus monkey, Xenopus tropicalis
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免疫原
Synthetic peptide within Human RABEP1 aa 812-862 (C terminal). The exact sequence is proprietary. NP_004694.2
Sequence:DVSEQVQRD FVKLSQTLQV QLERIRQADS LERIRAILND TKLTDINQLP ET
Database link: Q15276 -
阳性对照
- HeLa, 293T and NIH3T3 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab176578 was affinity purified using an epitope specific to RABEP1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176578于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 99 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 99 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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相关性
RABEP1 is a Rab effector protein acting as linker between gamma-adaptin, RAB4A and RAB5A. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates RABGEF1 mediated nucleotide exchange on RAB5A. -
细胞定位
Cytoplasm. Early endosome. Recycling endosome -
数据库链接
- Entrez Gene: 395930 Chicken
- Entrez Gene: 455255 Chimpanzee
- Entrez Gene: 504785 Cow
- Entrez Gene: 489451 Dog
- Entrez Gene: 9135 Human
- Omim: 603616 Human
- SwissProt: Q15276 Human
- SwissProt: O35551 Mouse
see all -
别名
- Neurocrescin antibody
- Rab GTPase binding effector protein 1 antibody
- RAB5EP antibody
see all
图片
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Western blot - Anti-RABEP1 antibody (ab176578)All lanes : Anti-RABEP1 antibody (ab176578) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysates at 50 µg
Lane 2 : HeLa whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 5 µg
Lane 4 : 293T whole cell lysates at 50 µg
Lane 5 : NIH3T3 whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 99 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-RABEP1 antibody (ab176578)Detection of RABEP1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176578 at 3 µg/mg lysate for IP (Lane 1). For WB detection ab176578 antibody which recognizes an upstream epitope was used at 0.4 µg/ml. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 3 seconds.
数据表及文件
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Datasheet download
文献 (0)
ab176578 尚未被引用在任何文献中。
