兔IgG,多克隆抗体-同型对照(ab37415)
概述
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产品名称
兔IgG,多克隆抗体-同型对照
参阅全部 Rabbit 亚类对照 -
特异性
This antibody has been selected to be an isotype control as it has no known specificity. Please note that the rabbit IgG Fc region may bind nonspecifically to human tissue. Rabbit IgG is an isotype control used to estimate the non-specific binding of target primary antibodies due to Fc binding or other protein-protein interactions. -
经测试应用
适用于: IHC-P, ICC/IF, ELISA, Flow Cyt, ChIP/Chipmore details -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Protein A-purified -
克隆
多克隆 -
同种型
IgG -
研究领域
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别名
- rabbit isotype control
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab37415于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. PubMed: 24961933
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 23995768
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| ELISA |
Use at an assay dependent concentration.
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| Flow Cyt |
Use 1µg for 106 cells.
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| ChIP/Chip |
Use at an assay dependent concentration. PubMed: 20673990
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| 说明 |
|---|
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IHC-P
Use at an assay dependent concentration. PubMed: 24961933 |
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ICC/IF
Use at an assay dependent concentration. PubMed: 23995768 |
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ELISA
Use at an assay dependent concentration. |
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Flow Cyt
Use 1µg for 106 cells. |
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ChIP/Chip
Use at an assay dependent concentration. PubMed: 20673990 |
图片
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Flow Cytometry - Rabbit IgG, polyclonal - Isotype Control (ab37415)Image from Hilbert T et al., PLoS One. 2013;8(5):e65024. Fig 3(A).; doi: 10.1371/journal.pone.0065024. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Histogram showing the fluorescence signal for ADRB2-FITC in PBMCs compared to an Isotype control.
PBMCs were generated from freshly-drawn blood from healthy human donors. After staining with antibodies against ADRB2 (ab36956) or an isotype control (ab37415), cells were incubated with FITC-labeled secondary anti-rabbit-IgG antibody, and ADRB2 expression was assessed by flow cytometry.
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ChIP - Rabbit IgG, polyclonal - Isotype Control (ab37415)Image from Popovic R et al., PLoS Genet.. 2014;10(9):e1004566. Fig 1(E).; doi: 10.1371/journal.pgen.1004566. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ChIP-qPCR for H3K36me2 on the BTF3 locus in NTKO and TKO cells. Methylation enrichment was tested 5 kb upstream of the TSS (5′) and at the TSS (right). ab37415 was used as the isotype contol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (236)
ab37415 被引用在 236 文献中.
- Zudeh G et al. PACSIN2 as a modulator of autophagy and mercaptopurine cytotoxicity: mechanisms in lymphoid and intestinal cells. Life Sci Alliance 6:N/A (2023). PubMed: 36596605
- Tseng HW et al. Interleukin-1 Is Overexpressed in Injured Muscles Following Spinal Cord Injury and Promotes Neurogenic Heterotopic Ossification. J Bone Miner Res 37:531-546 (2022). PubMed: 34841579
- Wang D et al. SPINK6 inhibits human airway serine proteases and restricts influenza virus activation. EMBO Mol Med 14:e14485 (2022). PubMed: 34826211
- Hämäläinen S et al. Carboxypeptidase A3 expression in canine mast cell tumors and tissue-resident mast cells. Vet Pathol 59:236-243 (2022). PubMed: 34894899
- Kamiya M et al. Targeting necroptosis in muscle fibers ameliorates inflammatory myopathies. Nat Commun 13:166 (2022). PubMed: 35013338