Rabbit IgG, monoclonal [EPR25A] - Isotype Control
Rabbit IgG, monoclonal [EPR25A] - Isotype Control
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- Recombinant
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(617 Publications)
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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Overlay histogram showing A549 (human lung carcinoma) cells stained with ab133557 (red line). The cells were fixed with 2% paraformaldehyde. The cells were then incubated with ab133557 at 1/60 dilution. The secondary antibody used was goat anti-rabbit IgG (FITC) at 1/150 dilution. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 530/30 bandpass filter.
Conjugated versions are available for this clone : Alexa Fluor® 488 (ab199091), Alexa Fluor® 647 (ab199093), R-PE (ab209478), APC (ab232814).
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometry overlay histogram showing left HeLa positive cells and right negative Jurkat stained with ab7753 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab7753) (1x 106 in 100μl at 0.2μg/ml (1/13150)) for 30min at 22°C.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C
Isotype control antibody (black line) was Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
This antibody gave a positive signal in HeLa Fixed with 4% formaldehyde (10 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometry overlay histogram showing left Ramos positive cells and right negative HEK293 stained with ab78237 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab78237) (1x 106 in 100μl at 0.2μg/ml (1/2500)) for 30min at 22°C.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C
Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
This antibody gave a positive signal in Ramos Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
- Flow Cyt (Intra)
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell, Left) / U937 (human histiocytic lymphoma monocyte, Right) cells labelling PU.1/Spi1 with ab302623 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control : HeLa (PMID : 27010793)
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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of SH-SY5Y (human neuroblastoma epithelial cell, Left) / HeLa (human cervix adenocarcinoma epithelial cell, Right) cells labelling CD98 with ab307587 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Low expression : SH-SY5Y .
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell, Left) / THP-1 (human monocytic leukemia monocyte, Right) cells labelling Neutrophil Elastase with ab314916 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling CRBN with ab315344 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HCT 116 (human colorectal carcinoma epithelial cell, Left) / No-GFP-CD16.NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell, Right) cells labelling cGAS with ab302617 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control : HCT 116 (PMID : 33790360)
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
- Flow Cyt (Intra)
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell, Left) / WI-38 (human fetal lung fibroblast, Right) cells labelling COL1A2 with ab308455 at 1/50 dilution (1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : HeLa.
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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling CCR9 with ab313773 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : Jurkat (PMID : 19525985). Gated on viable cells.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cells labelling TRIM8 with ab316149 at 1/50 dilution (1 ug)/(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of Wild-type HeLa (human cervix adenocarcinoma epithelial cell, Right) / SLC3A2 (CD98) knockout HeLa (Left) cells labelling CD98 with ab307587 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Positive staining on HeLa cells (ab255928), while no staining on CD98 knockout HeLa cells (ab265708).
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell, Left) / Jurkat (human T cell leukemia T lymphocyte, Right) cells labelling ETS1 with ab307672 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control : HepG2 (PMID : 30790340)
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized LNCaP (human prostate carcinoma epithelial cell, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling TCF7 with ab315392 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control : LNCaP.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling PU.1/Spi1 with ab302623 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SQSTM1 KO HAP1 (human SQSTM1 knockout chronic myelogenous leukemia near-haploid cell) / parental HAP1 cells labelling SQSTM1 with ab314504 at 1/5000 dilution (0.01 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labelling Ndufs1 with ab169540 at 1/20 dilution (1 ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-937 (Human histiocytic lymphoma monocyte) treated with 100ng/ml TPA for 24 hours, then 5μg/ml LPS for 4 hours, and add 300ng/ml BFA for another 3h (Red) / Untreated control (Green) cells labelling IL-8 with ab289967 at 1/500 dilution (0.1μg) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized T-47D (human ductal breast epithelial tumor epithelial cell, Left) / ARPE-19 (human eye epithelial cell, Right) cells labelling Fibrillin 1 with ab315384 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control : T-47D.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562 (human chronic myelogenous leukemia lymphoblast, Left) / HeLa (human cervical adenocarcinoma epithelial cell, Right) cells labelling BASP1 with ab312322 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : K-562 (PMID : 14701728).
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized (SMAD2 KO HeLa (human cervical adenocarcinoma epithelial cell) treated with 20ng/ml TGF-β1 for 1h (Red) and untreated SMAD2 KO HeLa (dotted red)) (Left) / (Parental HeLa treated with 20ng/ml TGF-β1 for 1h (Red) and untreated HeLa (dotted red)) (Right) cells labelling Smad2 (phospho S465 + S467) with ab316117 at 1/5000 dilution (0.01 ug)/Red and Dotted Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / Raji (human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD37 with ab300400 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Negative control : MCF7 (PMID : 33652767).
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (human neuroblastoma epithelial cell, Left) / HUVEC (human umbilical vein endothelial cell, Right) cells labelling Heparan Sulfate Proteoglycan 2/Perlecan with ab315240 at 1/500 dilution (0.1 ug, Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control : SH-SY5Y.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized IL8 KO PC-3 (IL-8 knock out human prostate adenocarcinoma epithelial cell) treated with 2µg/ml LPS for 5hours and 5µg/ml Brefeldin A for 5 hours (Megenta) or untreated with IL8 KO PC-3(Green)(Right) / PC-3 treated with 2µg/ml LPS for 5hours and 5µg/ml Brefeldin A for 5 hours(Megenta) or untreated with PC-3(Green)(Left) cells labelling IL-8 with ab242515 at 1/500 dilution (0.1µg) followed by a Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at a 1/5000 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of DLD-1 (human colorectal adenocarcinoma epithelial cell) (Right) / HeLa (human cervical adenocarcinoma epithelial cell) (Left) cells labelling Nectin-4 with ab317268 at 1/500 dilution (0.1ug)/red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Low expression : HeLa (PMID : 21901103).
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling WDR5 with ab307664 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling CKAP4 with ab302539 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) cells labelling MAVS with ab290729 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Molt-4 (human lymphoblastic leukemia T lymphoblast, Left) / A431 (human epidermoid carcinoma epithelial cell, Right) cells labelling Cytokeratin 18 with ab315096 at 1/500 dilution (0.1 ug, Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control : Molt-4.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling NSD3 with ab300489 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling SHMT2/SHMT with ab316328 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (human embryonic kidney epithelial cell) transfected with a Influenza A virus PB1 protein expression vector containing a myc tag cells labelling Influenza A virus PB1 protein with ab313887 at 1/500 dilution (0.1 µg)/Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells are co-stained with Myc tag conjugated to Alexa Fluor®647.
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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling TCR delta with ab313573 at 1/50 dilution (1 µg)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were co-stained with anti-CD3 conjugated to Alexa Fluor® 647. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or primary antibody.
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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CCR9 with ab313773 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells are co-stained with CD56 conjugated to BV421. Gated on viable cells.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HL-60 (human acute promyelocytic leukemia promyeloblast) treated with 1.25% DMSO for 5days) (Red) / untreated HL-60 (Green) cells labelling PADI4 / PAD4 with ab315269 at 1/5000 dilution (0.01 ug)/Red and Green compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A-204 (human muscle rhabdomyosarcoma cell, Left) / U266B1 (human multiple myeloma B lymphocyte, Right) cells labelling CPEB4 with ab315332 at 1/50 dilution (1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Low expression : A-204.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling FADS2 with ab314317 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)
Flow cytometry overlay histogram showing p53 in wild-type HAP1 (green line) and TP53 knockout HAP1 (red line) cells stained with ab32049. The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab32049) (1x 106 cells in 100μl at 0.2 μg/ml (1/440)) for 30min at 22°C. The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) was incubated at 1/4000 for 30min at 22°C. Isotype control antibody Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) was used at the same concentration and conditions as the primary antibody (wild-type HAP1 - black line, TP53 knockout HAP1 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity). Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. This antibody gave a positive signal in HAP1 Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
不同偶联物与剂型 (13)
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675 PerCP
PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control
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617 Alexa Fluor® 594
Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
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578 PE
PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control
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603 Alexa Fluor® 568
Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
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519 FITC
FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control
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Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free
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519 Alexa Fluor® 488
Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
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HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control
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665 Alexa Fluor® 647
Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
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421 Alexa Fluor® 405
Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
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565 Alexa Fluor® 555
Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
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660 APC
APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control
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Biotin Isotype Control [EPR25A]
反应性数据
产品详情
Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, ChIP-seq, Flow Cyt, ICC/IF, IHC-P, IP, and WB.
Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) was first used in a scientific publication in 2014 and has been cited over 617 times in peer reviewed journals. It's performance in Western blot, immunofluorescence, IHC and ChIP in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) is sold in 200 µg selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR25A - ab210849.
Antibody clone EPR25A is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, HRP, Alexa Fluor® 45, Alexa Fluor® 594, Alexa Fluor® 555, PE, Alexa Fluor® 568, PerCP, FITC, APC, Biotin, Alexa Fluor® 75 (ab19991, ab19993, ab19957, ab2815, ab28568, ab28569, ab29478, ab29613, ab22217, ab223339, ab232814, ab3273, ab32286).
One of the top cited IgG Isotype control antibodies in the market with >1000 citations. IgG Isotype Control antibodies are essential for multiple applications. Isotype Control ab172730 is suitable for ChIC/CUT&RUN-seq, IP, ICC/IF, IHC-P,Flow Cyt, WB. This negative control antibodies help distinguish specific antibody binding from non-specific interactions, ensuring reliable results. Optimize your research with high-quality IgG Isotype Control antibodies for precise data interpretation
Immunogen
Chemical / Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH forms a large complex composed of ~50 kDa subunits.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
性能和储存信息
形式
纯化工艺
存储溶液
运输条件
推荐的短期储存时间
推荐的短期储存条件
推荐的长期储存条件
分装信息
储存信息
产品实验方案
- Visit the General protocols
- Visit the Troubleshooting
- Download chicCutRunSequencingBooklet|en
靶点信息
文献 (617)
Recent publications for all applications. Explore the full list and refine your search
Molecular medicine reports 29: PubMed38099337
2023
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MedComm 4:e464 PubMed38107057
2023
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Journal of translational medicine 21:910 PubMed38098048
2023
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Neoplasma 70:659-669 PubMed38053376
2023
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Neoplasma 70:597-609 PubMed38053379
2023
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PeerJ 11:e16553 PubMed38077417
2023
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Nature genetics 56:100-111 PubMed38049663
2023
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Journal of experimental & clinical cancer research : CR 42:330 PubMed38049814
2023
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Journal of nanobiotechnology 21:459 PubMed38037135
2023
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Scientific reports 13:20262 PubMed37985889
2023
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Abcam Product Promise
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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