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Anti-Rab9抗体[Mab9] (ab2810)

  • Datasheet
  • SDS
Reviews (12)Q&A (1)References (23)

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Western blot - Anti-Rab9 antibody [Mab9] (ab2810)
  • Western blot - Anti-Rab9 antibody [Mab9] (ab2810)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody [Mab9] (ab2810)
  • Flow Cytometry - Anti-Rab9 antibody [Mab9] (ab2810)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody [Mab9] (ab2810)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody [Mab9] (ab2810)

Key features and details

  • Mouse monoclonal [Mab9] to Rab9
  • Suitable for: WB, Flow Cyt, IHC-P
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Rab9抗体[Mab9]
    参阅全部 Rab9 一抗
  • 描述

    小鼠单克隆抗体[Mab9] to Rab9
  • 宿主

    Mouse
  • 经测试应用

    适用于: WB, Flow Cyt, IHC-Pmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Hamster, Cow, Dog, Non human primates
  • 免疫原

    Recombinant full length protein corresponding to Dog Rab9.

  • 阳性对照

    • WB: MDA-MB-231, MCF7, HeLa, HEK293, Jurkat, HepG2 and K562 cell lysates. Flow Cyt: THP1 cells. IHC-P: Human tonsil and spleen tissues.
  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 纯度

    Protein G purified
  • Primary antibody说明

    Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.
  • 克隆

    单克隆
  • 克隆编号

    Mab9
  • 同种型

    IgG1
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Endosome
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

相关产品

  • Alternative Versions

    • Anti-Rab9 antibody [Mab9] - BSA and Azide free (ab237960)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • KO cell lines

    • Human RAB9A (Rab9) knockout HeLa cell line (ab265693)
  • KO cell lysates

    • Human RAB9A (Rab9) knockout HeLa cell lysate (ab257625)
  • Positive Controls

    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant human Rab9 protein (ab90106)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2810于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (4)
Use a concentration of 10 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P (3)
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
说明
WB
Use a concentration of 10 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能

    Involved in the transport of proteins between the endosomes and the trans Golgi network.
  • 序列相似性

    Belongs to the small GTPase superfamily. Rab family.
  • 细胞定位

    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Target information above from: UniProt accession P51151 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 511776 Cow
    • Entrez Gene: 403947 Dog
    • Entrez Gene: 9367 Human
    • Entrez Gene: 56382 Mouse
    • Entrez Gene: 84589 Rat
    • Omim: 300284 Human
    • SwissProt: P24408 Dog
    • SwissProt: P51151 Human
    • SwissProt: Q9R0M6 Mouse
    • SwissProt: Q99P75 Rat
    • Unigene: 495704 Human
    • Unigene: 728400 Human
    • Unigene: 25306 Mouse
    • Unigene: 35289 Rat
    see all
  • 别名

    • 2410064E05Rik antibody
    • AI195561 antibody
    • DmRab9 antibody
    • Rab 9 antibody
    • RAB 9A antibody
    • RAB9 member RAS oncogene family antibody
    • RAB9A antibody
    • RAB9A member RAS oncogene family antibody
    • RAB9A_HUMAN antibody
    • RAS ASSOCIATED PROTEIN RAB9 antibody
    • Ras related protein Rab 9A antibody
    • Ras-related protein Rab-9A antibody
    • Sid6061p antibody
    • Sid99 antibody
    see all

图片

  • Western blot - Anti-Rab9 antibody [Mab9] (ab2810)
    Western blot - Anti-Rab9 antibody [Mab9] (ab2810)
    All lanes : Anti-Rab9 antibody [Mab9] (ab2810) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : RAB9A knockout HeLa cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : MDA-MB-231 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution

    Predicted band size: 22 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab2810 observed at 25 kDa. Red - loading control ab181602 observed at 36 kDa. 

     ab2810 Anti-Rab9 antibody [Mab9] was shown to specifically react with Rab9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265693 (knockout cell lysate ab257625) was used.  Wild-type and Rab9 knockout samples were subjected to SDS-PAGE.  ab2810 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Rab9 antibody [Mab9] (ab2810)
    Western blot - Anti-Rab9 antibody [Mab9] (ab2810)
    All lanes : Anti-Rab9 antibody [Mab9] (ab2810) at 10 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa
    Additional bands at: 48 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody [Mab9] (ab2810)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody [Mab9] (ab2810)This image is courtesy of an Abreview submitted by Qin Wen

    ab2810 staining Rab9 in Mouse adult testes tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 60 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 1% BSA) for 15 hours at 4°C. A TRITC-conjugated Donkey anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Flow Cytometry - Anti-Rab9 antibody [Mab9] (ab2810)
    Flow Cytometry - Anti-Rab9 antibody [Mab9] (ab2810)

    Overlay histogram showing THP1 cells stained with ab2810 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2810, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody [Mab9] (ab2810)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody [Mab9] (ab2810)
    ab2810 (2µg/ml) staining Rab9 in human spleen, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and cell membrane staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody [Mab9] (ab2810)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody [Mab9] (ab2810)
    IHC image of Rab9 staining in human normal tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2810, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

实验方案

  • Mouse on Mouse staining protocol

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (23)

发表研究结果有使用 ab2810?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2810 被引用在 23 文献中.

  • Sasaki Y  et al. Estrogen Plays a Crucial Role in Rab9-Dependent Mitochondrial Autophagy, Delaying Arterial Senescence. J Am Heart Assoc 10:e019310 (2021). PubMed: 33719502
  • Salvany S  et al. Microglial recruitment and mechanisms involved in the disruption of afferent synaptic terminals on spinal cord motor neurons after acute peripheral nerve injury. Glia 69:1216-1240 (2021). PubMed: 33386754
  • Kim WY  et al. Atg7-dependent canonical autophagy regulates the degradation of aquaporin 2 in prolonged hypokalemia. Sci Rep 9:3021 (2019). PubMed: 30816234
  • Saito T  et al. An alternative mitophagy pathway mediated by Rab9 protects the heart against ischemia. J Clin Invest 129:802-819 (2019). PubMed: 30511961
  • Kucera A  et al. Spatiotemporal Resolution of Rab9 and CI-MPR Dynamics in the Endocytic Pathway. Traffic 17:211-29 (2016). PubMed: 26663757
View all Publications for this product

客户评价及客户问答

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提交评价 提交问题

1-10 of 13 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab9 antibody [Mab9]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (fibroblast)
Permeabilization
Yes - triton 0,1% 10min Room Temperature
Specification
fibroblast
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 12 2022

Western blot abreview for Anti-Rab9 antibody [Mab9]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
25 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 06 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Rab9 antibody [Mab9]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (adult testes)
Specification
adult testes
Fixative
4% PFA
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Qing Wen

Verified customer

提交于 Mar 27 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Rab9 antibody [Mab9]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pancreatic cancer)
Specification
pancreatic cancer
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - PBS + Triton 0.025%
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 09 2010

Immunohistochemistry (Frozen sections) abreview for Anti-Rab9 antibody [Mab9]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (pancreatic cancer)
Specification
pancreatic cancer
Fixative
Acetone
Permeabilization
Yes - PBS + Triton 0.025%
Blocking step
MOM kit blocking agaent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 06 2010

Immunohistochemistry (Frozen sections) abreview for Anti-Rab9 antibody [Mab9]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (pancreatic cancer)
Specification
pancreatic cancer
Fixative
Acetone+Methanol 1:1
Permeabilization
Yes - PBST
Blocking step
Vector Lab MOM kit #BMK-2202 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 04 2010

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab9 antibody [Mab9]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEFs)
Specification
MEFs
Fixative
Formaldehyde
Permeabilization
Yes - PBST
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 01 2010

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Rab9 antibody [Mab9]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (duodenum)
Specification
duodenum
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 16 2010

Western blot abreview for Anti-Rab9 antibody [Mab9]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (pancreatitis)
Loading amount
15 µg
Specification
pancreatitis
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 03 2010

Question

No bands in WB with human eye lens tissue.

Read More

Abcam community

Verified customer

Asked on May 25 2012

Answer

Thank you for your call today and for letting us know about the trouble with these antibodies.
As we discussed, I've issued credit note *** for the vial of ab51031. The full purchase price will be refunded back to your credit card pending approval from our accounting department. If you have any questions about this refund, please contact us.credits@abcam.com.
Also, I'm sending a free of charge vial of ab119344 which will replace the vial of ab2810. This will be delayed due to Monday's holiday, but I expect it to arrive on Wednesday of next week. Please keep me updated about the performance of this replacement antibody.
Please let me know if you have any questions or if there is anything else that we can do for you, and I'll be happy to help.

Read More

Abcam Scientific Support

回复于 May 25 2012

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研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
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