Name: Anti-Rab5 antibody [EPR5438] - Early Endosome Marker
Brand: RabMAb
SKU: ab109534
Rating: 4 (9 reviews)

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Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5438] to Rab5 - Early Endosome Marker
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human

Carrier Free

产品名称

Anti-Rab5抗体[EPR5438] - Early Endosome Marker
参阅全部 Rab5 一抗
描述

兔单克隆抗体[EPR5438] to Rab5 - Early Endosome Marker
宿主

Rabbit
经测试应用

适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- 293T, HeLa and Jurkat whole cell lysate (ab7899); Human brain tissue.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR5438
同种型

IgG
研究领域

Alternative Versions
- Anti-Rab5 antibody [EPR5438] - BSA and Azide free (ab234908)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HeLa membrane extract lysate (ab29547)
- Jurkat membrane extract lysate (ab30130)
Recombinant Protein
- Recombinant Human Rab5A protein (ab62956)

The Abpromise guarantee

Abpromise™承诺保证使用ab109534于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB	(5)	1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
IHC-P	(1)	1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

说明
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

应用说明

Is unsuitable for Flow Cyt,ICC/IF or IP.

功能

Required for the fusion of plasma membranes and early endosomes.
序列相似性

Belongs to the small GTPase superfamily. Rab family.
细胞定位

Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes.
Target information above from: UniProt accession P20339 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 5868 Human
- Entrez Gene: 271457 Mouse
- Entrez Gene: 64633 Rat
- Omim: 179512 Human
- SwissProt: P20339 Human
- SwissProt: Q9CQD1 Mouse
- SwissProt: M0RC99 Rat
- Unigene: 475663 Human
- Unigene: 329123 Mouse
see all
别名
- RAB 5 antibody
- RAB 5A antibody
- RAB5A antibody
- RAB5A member RAS oncogene family antibody
- RAB5A_HUMAN antibody
- RAS associated protein RAB5A antibody
- Ras related protein Rab 5A antibody
- Ras-related protein Rab-5A antibody
see all

Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Rab5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Hu brain whole cell lysate (20 µg)
Lane 4: Ms brain whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109534 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.
Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/1000 dilution

Lane 1 : Wild-type HAP1 lysate
Lane 2 : RAB5A Knockout HAP1 lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 24 kDa
Observed band size: 24 kDa

ab109534 was shown to react with RAB5A in wild-type HAP1 cells in Western blot with loss of signal observed in a RAB5A knockout cell line. Wild-type HAP1 and RAB5A knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab109534 overnight at 4 °C at a 1000 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

Immunohistochemical staining of paraffin embedded human pancreas with purified ab109534 at a working dilution of 1 in 1000. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/5000 dilution (purified)

Lane 1 : Mouse brain
Lane 2 : Rat brain

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 24 kDa
Observed band size: 24 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)This image is courtesy of an anonymous abreview.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Pancreas tissue sections (with ADM and PanIN lesions) from KC mice labeling Rab5 with ab109534 at 1/200 dilution. Tissue was fixed with paraformaldehyde and permeabilized with TritonX100 0.1% for 10 min at room temperature. Heat-mediated antigen retrieval was performed using TRIS-EDTA pH9 buffer. Tissue sections were blocked with 10% goat serum for 1 hour at 20°C. A polyclonal goat anti-rabbit Alexa Fluor 488 secondary antibody was used at 1/500 dilution.
See Abreview
Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/5000 dilution (purified)

Lane 1 : MCF7 cell lysate
Lane 2 : HEK293 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 24 kDa
Observed band size: 24 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/1000 dilution (unpurified)

Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 24 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

Immunohistochemical staining of Rab5 in paraffin embedded human brain tissue with unpurified ab109534, at 1/100 dilution.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab109534？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab109534 被引用在 16 文献中.

Zhou G et al. Exosome Mediated Cytosolic Cisplatin Delivery Through Clathrin-Independent Endocytosis and Enhanced Anti-cancer Effect via Avoiding Endosome Trapping in Cisplatin-Resistant Ovarian Cancer. Front Med (Lausanne) 9:810761 (2022). PubMed: 35592860
Andersen OM et al. A genetically modified minipig model for Alzheimer's disease with SORL1 haploinsufficiency. Cell Rep Med 3:100740 (2022). PubMed: 36099918
Frittoli E et al. Tissue fluidification promotes a cGAS-STING cytosolic DNA response in invasive breast cancer. Nat Mater N/A:N/A (2022). PubMed: 36581770
Rai P et al. IRGM1 links mitochondrial quality control to autoimmunity. Nat Immunol 22:312-321 (2021). PubMed: 33510463
Sharoar MG et al. Accumulation of saposin in dystrophic neurites is linked to impaired lysosomal functions in Alzheimer's disease brains. Mol Neurodegener 16:45 (2021). PubMed: 34215298

View all Publications for this product

客户评价及客户问答

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1-10 of 11 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (RPE-1 cell)

Permeabilization

Yes - 0.3% tritonx100

Specification

RPE-1 cell

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 27 2019

Application

Western blot

Sample

African green monkey Cell lysate - whole cell (Kidney (COS7 cells))

Gel Running Conditions

Reduced Denaturing (4-12% gel)

Loading amount

30 µg

Specification

Kidney (COS7 cells)

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 29 2017

Application

Western blot

Sample

Mouse Tissue lysate - whole (Brain)

Gel Running Conditions

Reduced Denaturing (4-12% gel)

Loading amount

30 µg

Specification

Brain

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 29 2017

Application

Western blot

Sample

Human Cell lysate - whole cell (Kidney (HEK293 cells))

Gel Running Conditions

Reduced Denaturing (4-12% gel)

Loading amount

30 µg

Specification

Kidney (HEK293 cells)

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 29 2017

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Murine Pancreatic tissue)

Permeabilization

Yes - Triton X100 0.01%

Specification

Murine Pancreatic tissue

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 25 2017

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Pancreas (with ADM and PanIN lesions) from KC mice)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: TRIS-EDTA pH9

Permeabilization

Yes - TritonX100 0.1% 10 min RT

Specification

Pancreas (with ADM and PanIN lesions) from KC mice

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 06 2016

Application

Western blot

Sample

Human Cell lysate - whole cell (Pancreatic Cancer)

Gel Running Conditions

Reduced Denaturing (12.5%)

Loading amount

30 µg

Specification

Pancreatic Cancer

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 01 2016

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HepaRG)

Specification

HepaRG

Fixative

Formaldehyde

Permeabilization

Yes - 0.1% TritonX-100

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. XXXXXX XXXXXX

Verified customer

提交于 May 16 2013

Application

Western blot

Sample

Human Cell lysate - whole cell (HepaRG)

Loading amount

20 µg

Specification

HepaRG

Gel Running Conditions

Reduced Denaturing (10%)

Blocking step

Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. XXXXXX XXXXXX

Verified customer

提交于 May 15 2013

Question

I would like to know what is the concentration of this antibody:
Rab5a 3768-1 Epitomics lot YH112425.

Abcam community

Verified customer

Asked on Apr 03 2013

Answer

The IgG concentration of Abcam ab109534 lot GR68319 (which is the same as Rab5a 3768-1 Epitomics lot YH112425) is 0.032 mg/ml. This is lower than the IgG concentration of most mouse monoclonal and rabbit polyclonal antibodies but is not unusual for rabbit monoclonals, which generally have much higher affinity for their targets than either mouse monoclonals or rabbit polyclonals.

Abcam Scientific Support

回复于 Apr 03 2013

1-10 of 11 Abreviews or Q&A

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (16)

客户评价及客户问答

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab5 antibody [EPR5438] - Early Endosome Marker

Western blot abreview for Anti-Rab5 antibody [EPR5438] - Early Endosome Marker

Western blot abreview for Anti-Rab5 antibody [EPR5438] - Early Endosome Marker

Western blot abreview for Anti-Rab5 antibody [EPR5438] - Early Endosome Marker

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab5 antibody [EPR5438] - Early Endosome Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Rab5 antibody [EPR5438] - Early Endosome Marker

Western blot abreview for Anti-Rab5 antibody [EPR5438] - Early Endosome Marker

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab5 antibody [EPR5438]

Western blot abreview for Anti-Rab5 antibody [EPR5438]