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AB109009

Anti-Rab4 抗体 [EPR3043] - Early Endosome Marker

Anti-Rab4 antibody [EPR3043] - Early Endosome Marker

  • KO Validated
  • RabMAb
  • Recombinant
  • Lab Essentials
  • 20ul selling size
  • 了解详情

1

(1 Review)

|

(15 Publications)

Rabbit Recombinant Monoclonal Rab4 antibody. Early Endosome marker. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 15 publications.

查看别名

RAB4, RAB4A, Ras-related protein Rab-4A

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labelling Rab4 with purified ab109009 at 1/250. Cells were fixed with 100% methanol and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Rab4 with Purified ab109009 at 1 : 170 dilution (10 μg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009)

ab109009 at 1/500 dilution staining Rab4 in HeLa by Immunofluorescence.

Flow Cytometry (Intracellular) - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Rab4 with Purified ab109009 at 1/200 dilution (1μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunoprecipitation - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009)
  • IP

Unknown

Immunoprecipitation - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009)

ab109009 (purified) at 1 : 80 dilution (2μg) immunoprecipitating Rab4 in MCF7 whole cell lysate.
Lane 1 (input) : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab109009 & MCF7 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab109009 in MCF7 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

Predicted band size: 24 kDa

false

Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009)
  • WB

Lab

Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Rab4 knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : Human fetal brain lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab109009 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109009 was shown to specifically react with Rab4 when Rab4 knockout samples were used. Wild-type and Rab4 knockout samples were subjected to SDS-PAGE. ab109009 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

Predicted band size: 24 kDa

false

Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009)
  • WB

Unknown

Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009)

All lanes:

Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

PC12 cell lysate at 10 µg

Lane 3:

Neuro 2a cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Lane 5:

SH SY5Y cell lysate at 10 µg

Lane 6:

Human fetal brain lysate at 10 µg

Predicted band size: 24 kDa

false

不同偶联物与剂型 (1)

  • Carrier free

    Anti-Rab4 antibody [EPR3043] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3043

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

Flow Cyt (Intra), WB, IP, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/170 - 1/1000", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Rab4 also known as Ras-related protein Rab-4A is a small GTPase with a molecular mass of approximately 24 kDa. This protein is expressed in various cell types predominantly in tissues with high endocytic activity. Rab4 primarily functions as an endosome marker cooperating with the endocytic machinery to regulate sorting and recycling of endosomes. It occupies key positions on early endosomes relevant to its role in vesicular trafficking and membrane transportation. Endosome staining typically identifies Rab4 as an essential component in understanding endocytosis dynamics.
Biological function summary

Rab4 coordinates the trafficking of cargo between endosomes and the plasma membrane. It usually forms part of a protein complex that governs the recycling pathway of internalized membrane proteins and lipids. Rab4 modulates various cellular processes by impacting the rapid recycling of receptors receptors often return to the cell surface in response to Rab4 regulation. This action is important for maintaining cellular homeostasis and efficient signal transduction serving as a checkpoint for receptor-mediated events.

Pathways

The protein plays significant roles in the endocytic recycling pathway and the insulin signaling pathway. Rab4's activity influences the trafficking cycle often interacting with other proteins such as Rab11 and Rab5 to ensure efficient cargo movement within cells. By facilitating swift endosome recycling Rab4 has a direct impact on intracellular signaling and trafficking pathways supporting a range of cellular activities. This concerted interaction accentuates its importance in managing endosomal traffic and maintaining cellular connectivity.

Rab4 has implications in neurodegenerative disease and cancer. Abnormal Rab4 function disrupts endosomal signaling contributing to the pathogenesis of conditions like Alzheimer’s disease where it interacts with proteins such as amyloid precursor protein (APP). In cancer the dysregulation of Rab4-associated pathways affects tumor progression and metastasis. Rab4's connection with proteins like EGFR (Epidermal Growth Factor Receptor) indicates its potential role in altering cancer cell behaviors and resistance mechanisms making it a focus of interest in therapeutic approaches.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state (PubMed : 15907487, PubMed : 16034420). Involved in protein transport (PubMed : 29425100). Plays a role in vesicular traffic. Mediates VEGFR2 endosomal trafficking to enhance VEGFR2 signaling (PubMed : 29425100). Acts as a regulator of platelet alpha-granule release during activation and aggregation of platelets (By similarity).
See full target information RAB4A
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文献 (15)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:328 PubMed40263294

2025

Opposing roles for GSK3β and ERK1-dependent phosphorylation of huntingtin during neuronal dysfunction and cell death in Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas J Krzystek,Rasika Rathnayake,Jia Zeng,Jing Huang,Gary Iacobucci,Michael C Yu,Shermali Gunawardena

NPJ Parkinson's disease 10:152 PubMed39174550

2024

Neuroprotection of low dose carbon monoxide in Parkinson's disease models commensurate with the reduced risk of Parkinson's among smokers.

Applications

Unspecified application

Species

Unspecified reactive species

K N Rose,M Zorlu,A Fassini,H Lee,W Cai,X Xue,S Lin,P Kivisakk,M A Schwarzschild,X Chen,S N Gomperts

Research (Washington, D.C.) 7:0418 PubMed39040920

2024

Melatonergic Signaling Sustains Food Allergy Through FcεRI Recycling.

Applications

Unspecified application

Species

Unspecified reactive species

Youxia Wang,Xinmei Zhang,Ifen Hung,Chunxue Liu,Wenkai Ren,Liangpeng Ge,Hao Wang

Cellular and molecular life sciences : CMLS 81:43 PubMed38217571

2024

Mechano-regulation by clathrin pit-formation and passive cholesterol-dependent tubules during de-adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Tithi Mandal,Arikta Biswas,Tanmoy Ghosh,Sreekanth Manikandan,Avijit Kundu,Ayan Banerjee,Dhrubaditya Mitra,Bidisha Sinha

iScience 25:105444 PubMed36310646

2022

Impairment of antiviral immune response and disruption of cellular functions by SARS-CoV-2 ORF7a and ORF7b.

Applications

Unspecified application

Species

Unspecified reactive species

Tránsito García-García,Raúl Fernández-Rodríguez,Natalia Redondo,Ana de Lucas-Rius,Sara Zaldívar-López,Blanca Dies López-Ayllón,José M Suárez-Cárdenas,Ángeles Jiménez-Marín,María Montoya,Juan J Garrido

Science advances 8:eabo4271 PubMed35704572

2022

CD38 reduces mitochondrial fitness and cytotoxic T cell response against viral infection in lupus patients by suppressing mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ping-Min Chen,Eri Katsuyama,Abhigyan Satyam,Hao Li,Jose Rubio,Sungwook Jung,Sylvia Andrzejewski,J David Becherer,Maria G Tsokos,Reza Abdi,George C Tsokos

Cell reports 38:110452 PubMed35235793

2022

Activity-dependent PI4P synthesis by PI4KIIIα regulates long-term synaptic potentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Guo,Chao-Hua Jiang,Chunfang Tong,Yanrui Yang,Zehua Wang,Sin Man Lam,Dou Wang,Rui Li,Guanghou Shui,Yun Stone Shi,Jia-Jia Liu

Glia 69:1216-1240 PubMed33386754

2021

Microglial recruitment and mechanisms involved in the disruption of afferent synaptic terminals on spinal cord motor neurons after acute peripheral nerve injury.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Salvany,Anna Casanovas,Lídia Piedrafita,Olga Tarabal,Sara Hernández,Jordi Calderó,Josep E Esquerda

Cells 9: PubMed32709066

2020

Distinct Roles for RAB10 and RAB29 in Pathogenic LRRK2-Mediated Endolysosomal Trafficking Alterations.

Applications

Unspecified application

Species

Unspecified reactive species

Pilar Rivero-Ríos,Maria Romo-Lozano,Belén Fernández,Elena Fdez,Sabine Hilfiker

Cardiovascular research 117:533-546 PubMed32044971

2020

Hepatocyte growth factor-regulated tyrosine kinase substrate is essential for endothelial cell polarity and cerebrovascular stability.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyang Yu,Jian Zeng,Jun Wang,Yaxiong Cui,Xiaopeng Song,Yizhe Zhang,Xuan Cheng,Ning Hou,Yan Teng,Yu Lan,Yeguang Chen,Xiao Yang
View all publications
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