重组Anti-RAB3B抗体[EPR12987] (ab177949)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12987] to RAB3B
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-RAB3B抗体[EPR12987]
参阅全部 RAB3B 一抗 -
描述
兔单克隆抗体[EPR12987] to RAB3B -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- 293T, HepG2, fetal brain and SW480 lysates; SW480 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12987 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177949于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
|
说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa. |
靶标
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功能
Protein transport. Probably involved in vesicular traffic. -
序列相似性
Belongs to the small GTPase superfamily. Rab family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5865 Human
- Entrez Gene: 69908 Mouse
- Entrez Gene: 81755 Rat
- Omim: 179510 Human
- SwissProt: P20337 Human
- SwissProt: Q9CZT8 Mouse
- SwissProt: Q63941 Rat
- Unigene: 123072 Human
see all -
别名
- Brain antigen RAB3B antibody
- RAB3B antibody
- RAB3B member RAS oncogene family antibody
see all
图片
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Intracellular flow cytometric analysis of permeabilized SW480 cellslabeling RAB3B with ab177949at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
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All lanes : Anti-RAB3B antibody [EPR12987] (ab177949) at 1/1000 dilution
Lane 1 : 293T lysate
Lane 2 : HepG2 lysate
Lane 3 : Fetal brain lysate
Lane 4 : SW480 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 25 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab177949 被引用在 2 文献中.
- Qi Z et al. N1-Methyladenosine modification of mRNA regulates neuronal gene expression and oxygen glucose deprivation/reoxygenation induction. Cell Death Discov 9:159 (2023). PubMed: 37173310
- Nowicka-Bauer K et al. Proteomic approach towards identification of seminal fluid biomarkers from individuals with severe oligozoospermia, cryptozoospermia and non-obstructive azoospermia: a pilot study. Transl Androl Urol 12:1497-1510 (2023). PubMed: 37969768