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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family

Anti-Rab3A抗体(ab3335)

  • Datasheet
  • SDS
Reviews (11) Submit a question References (15)

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Immunocytochemistry/ Immunofluorescence - Anti-Rab3A antibody (ab3335)
  • Western blot - Anti-Rab3A antibody (ab3335)

Key features and details

  • Rabbit polyclonal to Rab3A
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Rab3A抗体
    参阅全部 Rab3A 一抗
  • 描述

    兔多克隆抗体to Rab3A
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Rab3A aa 1-18.
    Sequence:

    MASATDSRYGQKESSDQN


    (Peptide available as ab4951)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: mouse brain, kidney, liver, rat brain, kidney, liver; ICC: HeLa cells
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • Primary antibody说明

    Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in co-ordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals and the best studied is rab 3A. Rab 3A is found to be abundant in presynaptic nerve terminals and is also found to be crucial in acrosomal exocytosis in human spermatozoa. Abnormal accumulation of rab 3A in the cytoplasm of Purkinje cells has been reported in the prion protein related Creutzfeldt-Jakob disease.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Rab3A protein (ab109964)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3335于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (7)
Use a concentration of 2 µg/ml.
ICC/IF (1)
Use a concentration of 2 µg/ml.
说明
WB
Use a concentration of 2 µg/ml.
ICC/IF
Use a concentration of 2 µg/ml.

靶标

  • 功能

    Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
  • 组织特异性

    Specifically expressed in brain.
  • 序列相似性

    Belongs to the small GTPase superfamily. Rab family.
  • 细胞定位

    Cell membrane.
  • Target information above from: UniProt accession P20336 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 5864 Human
    • Entrez Gene: 19339 Mouse
    • Entrez Gene: 25531 Rat
    • Omim: 179490 Human
    • SwissProt: P20336 Human
    • SwissProt: P63011 Mouse
    • SwissProt: P63012 Rat
    • Unigene: 27744 Human
    • Unigene: 5083 Mouse
    • Unigene: 44409 Rat
    see all
  • 别名

    • Rab 3A antibody
    • RAB 3A member RAS oncogene family antibody
    • Rab3a antibody
    • RAB3A member RAS oncogene family antibody
    • RAB3A_HUMAN antibody
    • RAS associated protein RAB 3A antibody
    • RAS associated protein RAB3A antibody
    • Ras related protein Rab 3A antibody
    • Ras related protein Rab3A antibody
    • Ras-related protein Rab-3A antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab3A antibody (ab3335)
    Immunocytochemistry/ Immunofluorescence - Anti-Rab3A antibody (ab3335)

    Immunofluorescence analysis of RAB3A was done on 70% confluent log phase HeLa (Human epithelial adenocarcinoma cell line) 
    cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3335 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300 dilution. Panel d is a merged image showing Cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

  • Western blot - Anti-Rab3A antibody (ab3335)
    Western blot - Anti-Rab3A antibody (ab3335)
    All lanes : Anti-Rab3A antibody (ab3335) at 2 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Mouse liver tissue lysate
    Lane 4 : Rat brain tissue lysate
    Lane 5 : Rat kidney tissue lysate
    Lane 6 : Rat liver tissue lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution


    A 27 kDa band corresponding to RAB3A was observed along with two uncharacterized band (*) at ~40 kD and 60 kDa across tissues tested. 

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (15)

发表研究结果有使用 ab3335?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3335 被引用在 15 文献中.

  • Astillero-Lopez V  et al. Neurodegeneration and astrogliosis in the entorhinal cortex in Alzheimer's disease: Stereological layer-specific assessment and proteomic analysis. Alzheimers Dement 18:2468-2480 (2022). PubMed: 35142030
  • Prasai B  et al. The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells. Nat Commun 12:3970 (2021). PubMed: 34172739
  • Wauters F  et al. LRRK2 mutations impair depolarization-induced mitophagy through inhibition of mitochondrial accumulation of RAB10. Autophagy 16:203-222 (2020). PubMed: 30945962
  • Connor-Robson N  et al. An integrated transcriptomics and proteomics analysis reveals functional endocytic dysregulation caused by mutations in LRRK2. Neurobiol Dis 127:512-526 (2019). PubMed: 30954703
  • Folsom TD  et al. Quantitative proteomics of forebrain subcellular fractions in fragile X mental retardation 1 knockout mice following acute treatment with 2-Methyl-6-(phenylethynyl)pyridine: Relevance to developmental study of schizophrenia. Synapse 73:e22069 (2019). PubMed: 30176067
View all Publications for this product

客户评价及客户问答

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1-10 of 11 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Rab3A antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain (Hippocampus/entorhinal))
Permeabilization
Yes - Citrate Buffer, Triton X-100
Specification
Brain (Hippocampus/entorhinal)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

SRA. PATRICIA VILLANUEVA ANGUITA

Verified customer

提交于 Nov 17 2021

Western blot abreview for Anti-Rab3A antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus)
Gel Running Conditions
Reduced Denaturing (13%)
Loading amount
20 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Sergi Bayod

Verified customer

提交于 Oct 07 2019

Western blot abreview for Anti-Rab3A antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 05 2015

Western blot abreview for Anti-Rab3A antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (brain tissue)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 05 2015

Immunoprecipitation abreview for Anti-Rab3A antibody

Good
Abreviews
Abreviews
Application
Immunoprecipitation
Immuno-precipitation step
Other - magnetic Dynabeads
Sample
Mouse Tissue lysate - whole (brain)
Specification
brain
Total protein in input
500 µg
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 10 2015

Western blot abreview for Anti-Rab3A antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
2 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Rat Tissue lysate - whole (hippocampus tissue lysate)
Specification
hippocampus tissue lysate
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 18 2014

Western blot abreview for Anti-Rab3A antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Human Tissue lysate - whole (BRAIN)
Specification
BRAIN
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 06 2013

Western blot abreview for Anti-Rab3A antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH3T3)
Loading amount
30 µg
Specification
NIH3T3
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 07 2012

Western blot abreview for Anti-Rab3A antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus)
Loading amount
30 µg
Specification
Hippocampus
Gel Running Conditions
Reduced Denaturing (12% TGS)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 19 2008

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab3A antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (CHO-K1)
Specification
CHO-K1
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 11 2008

1-10 of 11 Abreviews or Q&A

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