Anti-RAB27A抗体[1G7] (ab55667)
![Western blot - Anti-RAB27A antibody [1G7] (ab55667)](https://www.abcam.cn/ps/products/55/ab55667/Images/ab55667-276548-anti-rab27a-antibody-western-blot.jpg "Western blot - Anti-RAB27A antibody [1G7] (ab55667)")
Key features and details
- Mouse monoclonal [1G7] to RAB27A
- Suitable for: WB, IHC-P, ICC/IF, IP, IHC-FoFr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-RAB27A抗体[1G7]
参阅全部 RAB27A 一抗 -
描述
小鼠单克隆抗体[1G7] to RAB27A -
宿主
Mouse -
特异性
ab55667 is likely to cross-react with RAB27B as the immunogen has 60 % homology with RAB27B.
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经测试应用
适用于: WB, IHC-P, ICC/IF, IP, IHC-FoFrmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Rabbit
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免疫原
Recombinant fragment corresponding to Human RAB27A aa 122-221.
Sequence:YCENPDIVLCGNKSDLEDQRVVKEEEAIALAEKYGIPYFETSAANGTNIS QAIEMLLDLIMKRMERCVDKSWIPEGVVRSNGHASTDQLSEEKEKGACGC
Database link: P51159 -
阳性对照
- WB: HAP1, Jurkat, MCF7 whole cell lysate; HL-60 cells; 293 cells, IHC-P: Human lymphoma. ICC/IF: Cow Retinal Pigment Epithelium (RPE) cells
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常规说明
This product was changed from ascites to tissue culture supernatant on 13th Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4 -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
1G7 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab55667于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
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| IHC-P | (2) |
Use at an assay dependent concentration.
|
| ICC/IF | (3) |
Use at an assay dependent concentration.
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| IP | (1) |
Use at an assay dependent concentration.
See Abreview. |
| IHC-FoFr |
Use at an assay dependent concentration. PubMed: 21832089
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. See Abreview. |
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IHC-FoFr
Use at an assay dependent concentration. PubMed: 21832089 |
靶标
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功能
Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. -
组织特异性
Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells. -
疾病相关
Defects in RAB27A are a cause of Griscelli syndrome type 2 (GS2) [MIM:607624]. Griscelli syndrome is a rare autosomal recessive disorder that results in pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes. GS2 patients also develop an uncontrolled T-lymphocyte and macrophage activation syndrome, known as hemophagocytic syndrome, leading to death in the absence of bone marrow transplantation. Neurological impairment is present in some patients, likely as a result of hemophagocytic syndrome. -
序列相似性
Belongs to the small GTPase superfamily. Rab family. -
细胞定位
Membrane. Melanosome. Late endosome. Lysosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes to endosomal exocytic vesicles. - Information by UniProt
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数据库链接
- Entrez Gene: 5873 Human
- Entrez Gene: 11891 Mouse
- Entrez Gene: 50645 Rat
- Omim: 603868 Human
- SwissProt: P51159 Human
- SwissProt: Q9ERI2 Mouse
- SwissProt: P23640 Rat
- Unigene: 654978 Human
see all -
别名
- GS2 antibody
- GTP-binding protein Ram antibody
- HsT18676 antibody
see all
图片
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Western blot - Anti-RAB27A antibody [1G7] (ab55667)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: RAB27A knockout HAP1 whole cell lysate (20 µg)
Lane 3: Jurkat whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab55667 observed at 27 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab55667 was shown to specifically react with RAB27A when RAB27A knockout samples were used. Wild-type and RAB27A knockout samples were subjected to SDS-PAGE. Ab55667 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 2.5 ug/ml and 1/10000 dilution respectively. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) and IRDye® 680 Goat anti-Rabbit IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
This image was generated using the ascites version of the product.
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![Western blot - Anti-RAB27A antibody [1G7] (ab55667)](https://www.abcam.cn/ps/products/55/ab55667/Images/ab55667-275314-anti-rab27a-antibody-western-blot.jpg)
Western blot - Anti-RAB27A antibody [1G7] (ab55667)Anti-RAB27A antibody [1G7] (ab55667) + HL-60 cell lysate
Predicted band size: 25 kDaThis image was generated using the ascites version of the product.
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![Western blot - Anti-RAB27A antibody [1G7] (ab55667)](https://www.abcam.cn/ps/products/55/ab55667/Images/ab55667-275316-anti-rab27a-antibody-western-blot.jpg)
Western blot - Anti-RAB27A antibody [1G7] (ab55667)All lanes : Anti-RAB27A antibody [1G7] (ab55667)
Lane 1 : RAB27A over-expressed 293 cell line, cotransfected with RAB27A Validated Chimera RNAi
Lane 2 : Non-transfected control
Predicted band size: 25 kDaGAPDH (36.1 kDa) used as specificity and loading control.
This image was generated using the ascites version of the product.
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![Western blot - Anti-RAB27A antibody [1G7] (ab55667)](https://www.abcam.cn/ps/products/55/ab55667/Images/ab55667-275315-anti-rab27a-antibody-western-blot.jpg)
Western blot - Anti-RAB27A antibody [1G7] (ab55667)All lanes : Anti-RAB27A antibody [1G7] (ab55667)
Lane 1 : RAB27A transfected lysate(24.9 KDa)
Lane 2 : Non-transfected lysate
Predicted band size: 25 kDaThis image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A antibody [1G7] (ab55667)](https://www.abcam.cn/ps/products/55/ab55667/Images/ab55667-69015-anti-rab27a-antibody-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27A antibody [1G7] (ab55667)RAB27A antibody (ab55667) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymphoma. Heat induced antigen retrieval with Citrate buffer pH 6 has been performed prior to immunostaining.
This image was generated using the ascites version of the product.
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![Western blot - Anti-RAB27A antibody [1G7] (ab55667)](https://www.abcam.cn/ps/products/55/ab55667/Images/ab55667-69013-anti-rab27a-antibody-western-blot.jpg)
Western blot - Anti-RAB27A antibody [1G7] (ab55667)This image is courtesy of an Abreview submitted by Dr Vladimir MilenkovicAll lanes : Anti-RAB27A antibody [1G7] (ab55667) at 1/200 dilution
Lane 1 : whole cell lysate of HEK 293 transfected with empty pCDNA3 vector
Lane 2 : whole cell lysate of HEK 293 cells expressing Rab27a-GFP
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-mouse
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 30 minutesRab27a (25kDa)+GFP(27kDa)=52kDa
The blot was blocked with 5% milk for 30 minutes at 25°C and incubated with the primary antibody for 12 hours at 4°C.
This image was generated using the ascites version of the product.
数据表及文件
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SDS download
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Datasheet download
文献 (46)
ab55667 被引用在 46 文献中.
- Wang H et al. Expression and significant roles of the lncRNA NEAT1/miR-493-5p/Rab27A axis in ulcerative colitis. Immun Inflamm Dis 11:e814 (2023). PubMed: 37249278
- Park YJ et al. Senescent melanocytes driven by glycolytic changes are characterized by melanosome transport dysfunction. Theranostics 13:3914-3924 (2023). PubMed: 37554281
- Gao R et al. Cryptochrome 1 activation inhibits melanogenesis and melanosome transport through negative regulation of cAMP/PKA/CREB signaling pathway. Front Pharmacol 14:1081030 (2023). PubMed: 36814484
- Shin JM et al. Sulfisoxazole Elicits Robust Antitumour Immune Response Along with Immune Checkpoint Therapy by Inhibiting Exosomal PD-L1. Adv Sci (Weinh) 9:e2103245 (2022). PubMed: 34927389
- Saliba J et al. Loss of NFE2L3 protects against inflammation-induced colorectal cancer through modulation of the tumor microenvironment. Oncogene 41:1563-1575 (2022). PubMed: 35091681