Anti-Rab20抗体(ab105043)
Key features and details
- Rabbit polyclonal to Rab20
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Rab20抗体
参阅全部 Rab20 一抗 -
描述
兔多克隆抗体to Rab20 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 49- 200 of Human Rab20 (AAH26025).
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阳性对照
- WB: HeLa, HepG2 and HCT116 cell lysate IF: HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab105043于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100 - 1/500.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
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| 说明 |
|---|
|
ICC/IF
1/100 - 1/500. |
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WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
靶标
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功能
Plays a role in apical endocytosis/recycling. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. -
组织特异性
Low or absent expression in normal pancreas and stronger expression in 15 of 18 exocrine pancreatic adenocarcinomas (at protein level). -
序列相似性
Belongs to the small GTPase superfamily. Rab family. -
细胞定位
Golgi apparatus. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane. Highly enriched on apical endocytic structures in polarized epithelial cells of kidney proximal tubules (By similarity). Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211). - Information by UniProt
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数据库链接
- Entrez Gene: 55647 Human
- SwissProt: Q9NX57 Human
- Unigene: 743563 Human
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别名
- FLJ20429 antibody
- RAB20 antibody
- RAB20 member RAS oncogene family antibody
see all
图片
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Western blot - Anti-Rab20 antibody (ab105043)Anti-Rab20 antibody (ab105043) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Rab20 antibody (ab105043)ab105043, at a 1/200 dilution, staining Rab20 in methanol-fixed HeLa cells. Right image is costained with Hoechst 33342.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab105043 尚未被引用在任何文献中。