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AB327718

Anti-RAB13 antibody [MJF-D31248-592] - BSA and Azide free (Capture)

Anti-RAB13 antibody [MJF-D31248-592] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal RAB13 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

GIG4, RAB13, Ras-related protein Rab-13, Cell growth-inhibiting gene 4 protein

3 Images
Sandwich ELISA - Anti-RAB13 antibody [MJF-D31248-592] - BSA and Azide free (Capture) (AB327718)
  • sELISA

Lab

Sandwich ELISA - Anti-RAB13 antibody [MJF-D31248-592] - BSA and Azide free (Capture) (AB327718)

Sandwich ELISA with the capture antibody ab327718 dilution at 2 μg/mL and detector antibody ab327719 dilution at 0.5 μg/mL after biotin conjugation.

Sandwich ELISA - Anti-RAB13 antibody [MJF-D31248-592] - BSA and Azide free (Capture) (AB327718)
  • sELISA

Lab

Sandwich ELISA - Anti-RAB13 antibody [MJF-D31248-592] - BSA and Azide free (Capture) (AB327718)

Interpolated concentration of native RAB13 was measured at different sample concentrations. Undiluted samples are as follows : HEK293T cell extract (500 µg/mL), SH-SY5Y cell extract (200 µg/mL) and U-87 MG cell extract (200 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-RAB13 antibody [MJF-D31248-592] - BSA and Azide free (Capture) (AB327718)
  • sELISA

Lab

Sandwich ELISA - Anti-RAB13 antibody [MJF-D31248-592] - BSA and Azide free (Capture) (AB327718)

Interpolated concentrations of native RAB13 in human control wild type HEK293T (human embryonic kidney epithelial cell) and RAB13 knockout HEK293T cell based on 500 µg/mL extract loads. The concentrations of RAB13 were measured in duplicate and interpolated from the RAB13 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RAB13 concentration was determined to be 4981.0 pg/mL in wild type HEK293T extract (Human wild-type HEK293T cell line ab255449) and undetectable in RAB13 knockout HEK293T extract (Human RAB13 knockout HEK293T cell line ab266843).

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

MJF-D31248-592

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

<p>This antibody is specific to human RAB13 and shows no cross-reactivity with human RAB8A, RAB8B, or RAB10&#46; The antibody pair, consisting of ab327718 as the capture and ab327719 as the detector, exhibits no cross-reactivity with human RAB8A, RAB8B, or RAB10&#46;</p>

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab327718 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human RAB13. The recommended pair for sandwich ELISA is:

Capture: ab327718, Human RAB13 Capture Antibody (unconjugated)

Detector: ab327719, Human RAB13 Detector Antibody (unconjugated)

The reference range value is 78.1-10000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB13 is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis.
See full target information RAB13
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Abcam Product Promise

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