Anti-RAB11B抗体(ab3612)
Key features and details
- Rabbit polyclonal to RAB11B
- Suitable for: ICC, WB
- Reacts with: Dog, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-RAB11B抗体
参阅全部 RAB11B 一抗 -
描述
兔多克隆抗体to RAB11B -
宿主
Rabbit -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Dog, Human
预测可用于: Mouse, Rat, Rabbit, Chicken, Cow, Pig, Non human primates
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免疫原
Synthetic peptide corresponding to Human RAB11B aa 1-100.
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阳性对照
- WB: canine MDCK, human PC3; ICC: HeLa
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
1/50 - 1/200.
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| WB | (2) |
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 24 kDa). By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract.
Another less prominent, unknown band is also detected by this antibody at ~35 kDa.
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| 说明 |
|---|
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ICC
1/50 - 1/200. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 24 kDa). By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kDa. |
靶标
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功能
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis. -
序列相似性
Belongs to the small GTPase superfamily. Rab family. -
翻译后修饰
Citrullinated by PADI4. -
细胞定位
Recycling endosome membrane. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasmic vesicle, phagosome membrane. Recruited to phagosomes containing S.aureus. - Information by UniProt
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数据库链接
- Entrez Gene: 420063 Chicken
- Entrez Gene: 532723 Cow
- Entrez Gene: 9230 Human
- Entrez Gene: 19326 Mouse
- Entrez Gene: 100513782 Pig
- Entrez Gene: 79434 Rat
- Omim: 604198 Human
- SwissProt: Q3MHP2 Cow
see all -
别名
- GTP-binding protein ypt3 antibody
- H YPT3 antibody
- RAB11B antibody
see all
图片
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Immunocytochemistry - Anti-RAB11B antibody (ab3612)
Immunocytochemical analysis of 4% paraformaldeehyde-fixed 0.1% Triton X-100 permeabilized HeLa cells treated/untreated with actinomycin D. Rab11B is stained with ab3612 at a 1/100 dilution and a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate at a 1/500 dilution (green). Nuclear counterstaining is using Hoechst 33342 (blue).
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Western blot - Anti-RAB11B antibody (ab3612)Anti-RAB11B antibody (ab3612) at 1/500 dilution + MDCK (canine kidney cell line) whole cell lysate at 20 µg
Predicted band size: 24 kDa -
Western blot - Anti-RAB11B antibody (ab3612)ab3612 used on PC3 cell extract.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (33)
ab3612 被引用在 33 文献中.
- Solinger JA et al. FERARI and cargo adaptors coordinate cargo flow through sorting endosomes. Nat Commun 13:4620 (2022). PubMed: 35941155
- Oh Y et al. SPIN90 Deficiency Ameliorates Amyloid β Accumulation by Regulating APP Trafficking in AD Model Mice. Int J Mol Sci 23:N/A (2022). PubMed: 36142484
- Barbet G et al. TAP dysfunction in dendritic cells enables noncanonical cross-presentation for T cell priming. Nat Immunol 22:497-509 (2021). PubMed: 33790474
- Barreiro-Alonso A et al. HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis through NuRD, THOC and Septin Complexes. Cancers (Basel) 13:N/A (2021). PubMed: 34572914
- Wang H et al. Unraveling GLUT-mediated transcytosis pathway of glycosylated nanodisks. Asian J Pharm Sci 16:120-128 (2021). PubMed: 33613735