重组Anti-RAB11-FIP2抗体[EPR12294] (ab174313)
![Western blot - Anti-RAB11-FIP2 antibody [EPR12294] (ab174313)](https://www.abcam.cn/ps/products/174/ab174313/Images/ab174313-199219-anti-rab11-fip2-antibody-epr12294-western-blot.jpg "Western blot - Anti-RAB11-FIP2 antibody [EPR12294] (ab174313)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12294] to RAB11-FIP2
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-RAB11-FIP2抗体[EPR12294]
参阅全部 RAB11-FIP2 一抗 -
描述
兔单克隆抗体[EPR12294] to RAB11-FIP2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IPmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human RAB11-FIP2. The exact sequence is proprietary.
Database link: Q7L804 -
阳性对照
- 293T and HeLa cell lysates; permeabilized HeLa cells; Immunoprecipitation pellet from 293T cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12294 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab174313于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
|
| IP |
1/10 - 1/100.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 58 kDa. |
|
IP
1/10 - 1/100. |
靶标
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功能
A Rab11 effector binding preferentially phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and phosphatidic acid (PA) and acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Involved in insulin granule exocytosis. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Required in a complex with MYO5B and RAB11 for the transport of NPC1L1 to the plasma membrane. Also acts as a regulator of cell polarity. -
序列相似性
Contains 1 C2 domain.
Contains 1 FIP-RBD domain. -
翻译后修饰
Phosphorylation at Ser-227 by MARK2 regulates epithelial cell polarity. -
细胞定位
Cell membrane. Recycling endosome membrane. Translocates with RAB11A from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 22841 Human
- Omim: 608599 Human
- SwissProt: Q7L804 Human
- Unigene: 173656 Human
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别名
- KIAA0941 antibody
- NRip11 antibody
- RAB11 family interacting protein 2 (class I) antibody
see all
图片
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Western blot - Anti-RAB11-FIP2 antibody [EPR12294] (ab174313)All lanes : Anti-RAB11-FIP2 antibody [EPR12294] (ab174313) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 58 kDa -
![Flow Cytometry (Intracellular) - Anti-RAB11-FIP2 antibody [EPR12294] (ab174313)](https://www.abcam.cn/ps/products/174/ab174313/Images/ab174313-199218-anti-rab11-fip2-antibody-epr12294-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-RAB11-FIP2 antibody [EPR12294] (ab174313)Intracellular flow cytometric analysis of permeabilized HeLa cells using ab174313 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
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![Immunoprecipitation - Anti-RAB11-FIP2 antibody [EPR12294] (ab174313)](https://www.abcam.cn/ps/products/174/ab174313/Images/ab174313-199217-anti-rab11-fip2-antibody-epr12294-immunoprecipitation.jpg)
Immunoprecipitation - Anti-RAB11-FIP2 antibody [EPR12294] (ab174313)Western blot analysis of immunoprecipitation pellet from 293T cell lysate (lane 1) or 1XPBS (negative control) (lane 2) using ab174313 at a 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Anti-RAB11-FIP2 antibody [EPR12294] (ab174313)](https://www.abcam.cn/ps/products/174/ab174313/Images/ab174313-4-benefits-of-recombinant-antibodies.png)
Anti-RAB11-FIP2 antibody [EPR12294] (ab174313)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab174313 被引用在 1 文献中.
- He K et al. Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules. Nat Commun 9:3310 (2018). PubMed: 30120249