Anti-QSOX2抗体(ab121376)
Key features and details
- Rabbit polyclonal to QSOX2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-QSOX2抗体
参阅全部 QSOX2 一抗 -
描述
兔多克隆抗体to QSOX2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
internal sequence amino acids 504-633 of Human QSOX2.
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阳性对照
- Human epididymis tissue; RT4 and U-251 MG cell lysate; Human Plasma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121376于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/250 - 1/500.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| 说明 |
|---|
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/250 - 1/500. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
靶标
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功能
Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins. Also seems to play a role in regulating the sensitization of neuroblastoma cells for interferon-gamma-induced apoptosis. -
组织特异性
Expressed in pancreas, brain, placenta, kidney, heart and fetal tissues. Weakly expressed in lung, liver and skeletal muscles. -
序列相似性
Belongs to the quiescin-sulfhydryl oxidase (QSOX) family.
Contains 1 ERV/ALR sulfhydryl oxidase domain.
Contains 1 thioredoxin domain. -
细胞定位
Membrane. Secreted. Cell membrane. Nucleus membrane. Seems to be predominantly targeted to the nuclear and outer plasma membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 169714 Human
- Omim: 612860 Human
- SwissProt: Q6ZRP7 Human
- Unigene: 657864 Human
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别名
- DKFZp762A2013 antibody
- Neuroblastoma-derived sulfhydryl oxidase antibody
- QSCN6L1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-QSOX2 antibody (ab121376)Immunofluorescent staining of Human cell line U-2 OS shows positivity in golgi apparatus and vesicles. Recommended concentration of ab121376 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-QSOX2 antibody (ab121376)ab121376 at 1/75 dilution staining QSOX2 in paraffin-embedded Human epididymis tissue by Immunohistochemistry. -
Western blot - Anti-QSOX2 antibody (ab121376)All lanes : Anti-QSOX2 antibody (ab121376) at 1/250 dilution
Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2 : RT-4 cell lysate.
Lane 3 : U-251 MG cell lysate.
Lane 4 : Human Plasma
Lane 5 : Human Liver lysate.
Lane 6 : Human Tonsil lysate.
Developed using the ECL technique.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab121376 被引用在 3 文献中.
- Wang TE et al. Testosterone regulation on quiescin sulfhydryl oxidase 2 synthesis in the epididymis. Reproduction 161:593-602 (2021). PubMed: 33784244
- Li Y et al. QSOX2 Is an E2F1 Target Gene and a Novel Serum Biomarker for Monitoring Tumor Growth and Predicting Survival in Advanced NSCLC. Front Cell Dev Biol 9:688798 (2021). PubMed: 34350181
- Kuo YW et al. Identification, characterization and purification of porcine Quiescin Q6-Sulfydryl Oxidase 2 protein. BMC Vet Res 13:205 (2017). PubMed: 28662655