Anti-Pyruvate Dehydrogenase E2抗体(ab126224)
Key features and details
- Rabbit polyclonal to Pyruvate Dehydrogenase E2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Pyruvate Dehydrogenase E2抗体
参阅全部 Pyruvate Dehydrogenase E2 一抗 -
描述
兔多克隆抗体to Pyruvate Dehydrogenase E2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to a region within amino acids 310-548 of Human Pyruvate Dehydrogenase E2 (UniProt ID: P10515).
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阳性对照
- 293T, A431, HeLa and HepG2 whole cell lysate (ab7900)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126224于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/5000 - 1/20000. Predicted molecular weight: 69 kDa.
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| 说明 |
|---|
|
WB
1/5000 - 1/20000. Predicted molecular weight: 69 kDa. |
靶标
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功能
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). -
疾病相关
Note=Primary biliary cirrhosis is a chronic, progressive cholestatic liver disease characterized by the presence of antimitochondrial autoantibodies in patients' serum. It manifests with inflammatory obliteration of intra-hepatic bile duct, leading to liver cell damage and cirrhosis. Patients with primary biliary cirrhosis show autoantibodies against the E2 component of pyruvate dehydrogenase complex.
Defects in DLAT are the cause of pyruvate dehydrogenase E2 deficiency (PDHE2 deficiency) [MIM:245348]; also known as lactic acidemia due to defect of E2 lipoyl transacetylase of the pyruvate dehydrogenase complex. Pyruvate dehydrogenase (PDH) deficiency is a major cause of primary lactic acidosis and neurological dysfunction in infancy and early childhood. In this form of PDH deficiency episodic dystonia is the major neurological manifestation, with other more common features of pyruvate dehydrogenase deficiency, such as hypotonia and ataxia, being less prominent. -
序列相似性
Belongs to the 2-oxoacid dehydrogenase family.
Contains 2 lipoyl-binding domains. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 1737 Human
- Omim: 608770 Human
- SwissProt: P10515 Human
- Unigene: 335551 Human
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别名
- 70 kDa mitochondrial autoantigen of primary biliary cirrhosis antibody
- Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex antibody
- Dihydrolipoamide S Acetyltransferase antibody
see all
图片
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Western blot - Anti-Pyruvate Dehydrogenase E2 antibody (ab126224)All lanes : Anti-Pyruvate Dehydrogenase E2 antibody (ab126224) at 1/10000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 69 kDa
7.5% SDS PAGE
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab126224 被引用在 4 文献中.
- Hossain AJ et al. Association of Phosphorylated Pyruvate Dehydrogenase with Pyruvate Kinase M2 Promotes PKM2 Stability in Response to Insulin. Int J Mol Sci 24:N/A (2023). PubMed: 37761999
- Hossain AJ et al. Pyruvate Dehydrogenase A1 Phosphorylated by Insulin Associates with Pyruvate Kinase M2 and Induces LINC00273 through Histone Acetylation. Biomedicines 10:N/A (2022). PubMed: 35740278
- Ward NP et al. Nicotinamide nucleotide transhydrogenase regulates mitochondrial metabolism in NSCLC through maintenance of Fe-S protein function. J Exp Med 217:N/A (2020). PubMed: 32196080
- Osinalde N et al. Changes in Gab2 phosphorylation and interaction partners in response to interleukin (IL)-2 stimulation in T-lymphocytes. Sci Rep 6:23530 (2016). WB . PubMed: 27025927